{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T14:05:26Z","timestamp":1785679526450,"version":"3.56.0"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives:<\/jats:title>\n                  <jats:p>To identify useful biomarkers for differentiating between malignant mesothelioma (MM) and reactive mesothelial cells (RMCs).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods:<\/jats:title>\n                  <jats:p>Formalin-fixed, paraffin-embedded (FFPE) tissues from 34 MM and 40 RMC samples were analyzed using immunohistochemistry, and the findings were compared.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results:<\/jats:title>\n                  <jats:p>Positive markers for MM included insulin-like growth factor 2 messenger RNA binding protein 3 (IMP3), glucose transporter 1 (GLUT1), epithelial membrane antigen (EMA), and CD146, which showed sensitivities of 94%, 85%, 79%, and 71% and specificities of 78%, 100%, 88%, and 98%, respectively. In sarcomatoid MM, EMA had significantly lower expression than did IMP3, GLUT1, and CD146 (P &amp;lt; .001). The areas under receiver operating characteristic curves were the highest for IMP3 (0.95), followed by GLUT1 (0.93). When the optimal cutoff points for IMP3 (30%) and GLUT1 (10%) were used, the sensitivity of IMP3 and GLUT1 for MM was 100%, and the specificity of both for MM was 95%.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions:<\/jats:title>\n                  <jats:p>The combination of IMP3 and GLUT1 is most appropriate for distinguishing MM from RMC using FFPE sections.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1309\/ajcp5knl7qtellyi","type":"journal-article","created":{"date-parts":[[2013,12,16]],"date-time":"2013-12-16T19:22:14Z","timestamp":1387221734000},"page":"85-93","source":"Crossref","is-referenced-by-count":53,"title":["Comparative Immunohistochemical Analysis of IMP3, GLUT1, EMA, CD146, and Desmin for Distinguishing Malignant Mesothelioma From Reactive Mesothelial Cells"],"prefix":"10.1093","volume":"141","author":[{"given":"Hiroshi","family":"Minato","sequence":"first","affiliation":[{"name":"1Department of Pathology and Laboratory Medicine, Kanazawa Medical University, Uchinada, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nozomu","family":"Kurose","sequence":"additional","affiliation":[{"name":"1Department of Pathology and Laboratory Medicine, Kanazawa Medical University, Uchinada, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mana","family":"Fukushima","sequence":"additional","affiliation":[{"name":"1Department of Pathology and Laboratory Medicine, Kanazawa Medical University, Uchinada, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Nojima","sequence":"additional","affiliation":[{"name":"1Department of Pathology and Laboratory Medicine, Kanazawa Medical University, Uchinada, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuo","family":"Usuda","sequence":"additional","affiliation":[{"name":"2Department of Thoracic Surgery, Kanazawa Medical University, Uchinada, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoyasu","family":"Sagawa","sequence":"additional","affiliation":[{"name":"2Department of Thoracic Surgery, Kanazawa Medical University, Uchinada, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsutomu","family":"Sakuma","sequence":"additional","affiliation":[{"name":"2Department of Thoracic Surgery, Kanazawa Medical University, Uchinada, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akishi","family":"Ooi","sequence":"additional","affiliation":[{"name":"3Department of Molecular Pathology, Kanazawa University, Kanazawa, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isao","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"4Department of General and Cardiothoracic Surgery, Kanazawa University, Kanazawa, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Oda","sequence":"additional","affiliation":[{"name":"4Department of General and Cardiothoracic Surgery, Kanazawa University, Kanazawa, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiko","family":"Arano","sequence":"additional","affiliation":[{"name":"5Department of Surgery, KKR Hokuriku Hospital, Kanazawa, Ishikawa, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junzo","family":"Shimizu","sequence":"additional","affiliation":[{"name":"5Department of Surgery, KKR Hokuriku Hospital, Kanazawa, Ishikawa, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,1,1]]},"reference":[{"key":"2023012006334448400_b1-1410085","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1056\/NEJMra050152","article-title":"Advances in malignant mesothelioma","volume":"353","author":"Robinson","year":"2005","journal-title":"N Engl J Med"},{"key":"2023012006334448400_b2-1410085","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1378\/chest.08-2665","article-title":"Malignant pleural mesothelioma: an update on biomarkers and treatment","volume":"136","author":"Ray","year":"2009","journal-title":"Chest"},{"key":"2023012006334448400_b3-1410085","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1002\/(SICI)1096-9896(199910)189:2<251::AID-PATH412>3.0.CO;2-F","article-title":"The use of histological and immunohistochemical markers to distinguish pleural malignant mesothelioma and in situ mesothelioma from reactive mesothelial hyperplasia and reactive pleural fibrosis","volume":"189","author":"Cury","year":"1999","journal-title":"J Pathol"},{"key":"2023012006334448400_b4-1410085","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1097\/00000478-200009000-00001","article-title":"The separation of benign and malignant mesothelial proliferations","volume":"24","author":"Churg","year":"2000","journal-title":"Am J Surg Pathol"},{"key":"2023012006334448400_b5-1410085","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1111\/j.1365-2559.2006.02442.x","article-title":"Sensitivity and specificity of immunohistochemical antibodies used to distinguish between benign and malignant pleural disease: a systematic review of published reports","volume":"49","author":"King","year":"2006","journal-title":"Histopathology"},{"key":"2023012006334448400_b6-1410085","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/modpathol.3800732","article-title":"Immunohistochemical detection of GLUT-1 can discriminate between reactive mesothelium and malignant mesothelioma","volume":"20","author":"Kato","year":"2007","journal-title":"Mod Pathol"},{"key":"2023012006334448400_b7-1410085","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.1038\/modpathol.2010.134","article-title":"Immunocytochemistry of CD146 is useful to discriminate between malignant pleural mesothelioma and reactive mesothelium","volume":"23","author":"Sato","year":"2010","journal-title":"Mod Pathol"},{"key":"2023012006334448400_b8-1410085","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1097\/PAS.0b013e318218985b","article-title":"Oncofetal protein IMP3, a new diagnostic biomarker to distinguish malignant mesothelioma from reactive mesothelial proliferation","volume":"35","author":"Shi","year":"2011","journal-title":"Am J Surg Pathol"},{"key":"2023012006334448400_b9-1410085","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.humpath.2010.06.003","article-title":"The use of insulin like-growth factor II messenger RNA binding protein-3 in diagnostic pathology","volume":"42","author":"Findeis-Hosey","year":"2011","journal-title":"Hum Pathol"},{"key":"2023012006334448400_b10-1410085","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1002\/cncr.23254","article-title":"Utility of anti-L523S antibody in the diagnosis of benign and malignant serous effusions","volume":"114","author":"Hanley","year":"2008","journal-title":"Cancer"},{"key":"2023012006334448400_b11-1410085","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/dc.21398","article-title":"Diagnostic usefulness of EMA, IMP3, and GLUT-1 for the immunocytochemical distinction of malignant cells from reactive mesothelial cells in effusion cytology using cytospin preparations","volume":"39","author":"Ikeda","year":"2011","journal-title":"Diagn Cytopathol"},{"key":"2023012006334448400_b12-1410085","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1146\/annurev.nu.16.070196.001315","article-title":"Structure, function, and regulation of the mammalian facilitative glucose transporter gene family","volume":"16","author":"Olson","year":"1996","journal-title":"Annu Rev Nutr"},{"key":"2023012006334448400_b13-1410085","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1309\/AJCPP5R2ZJZKCLWN","article-title":"The diagnostic utility of p16 FISH and GLUT-1 immunohistochemical analysis in mesothelial proliferations","volume":"135","author":"Monaco","year":"2011","journal-title":"Am J Clin Pathol"},{"key":"2023012006334448400_b14-1410085","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1309\/AJCPWFW7O1FVFLKT","article-title":"Usefulness of EMA, GLUT-1, and XIAP for the cytologic diagnosis of malignant mesothelioma in body cavity fluids","volume":"131","author":"Shen","year":"2009","journal-title":"Am J Clin Pathol"},{"key":"2023012006334448400_b15-1410085","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1158\/0008-5472.CAN-08-1363","article-title":"Identification of MCAM\/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma","volume":"69","author":"Bidlingmaier","year":"2009","journal-title":"Cancer Res"},{"key":"2023012006334448400_b16-1410085","first-page":"1098","article-title":"A new Mel-CAM (CD146)\u2013specific monoclonal antibody, MN-4, on paraffin-embedded tissue","volume":"11","author":"Shih","year":"1998","journal-title":"Mod Pathol"},{"key":"2023012006334448400_b17-1410085","first-page":"13","article-title":"The use of relative operating characteristic (ROC) curves in test performance evaluation","volume":"110","author":"Beck","year":"1986","journal-title":"Arch Pathol Lab Med"},{"key":"2023012006334448400_b18-1410085","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1002\/dc.22821","article-title":"CD147 immunohistochemistry discriminates between reactive mesothelial cells and malignant mesothelioma","volume":"40","author":"Pinheiro","year":"2012","journal-title":"Diagn Cytopathol"},{"key":"2023012006334448400_b19-1410085","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1002\/cncr.23413","article-title":"9p21 Deletion in the diagnosis of malignant mesothelioma in serous effusions additional to immunocytochemistry, DNA-ICM, and AgNOR analysis","volume":"114","author":"Onofre","year":"2008","journal-title":"Cancer"},{"key":"2023012006334448400_b20-1410085","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1038\/modpathol.2008.45","article-title":"Diagnostic importance of 9p21 homozygous deletion in malignant mesotheliomas","volume":"21","author":"Chiosea","year":"2008","journal-title":"Mod Pathol"},{"key":"2023012006334448400_b21-1410085","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1111\/j.1440-1827.2010.02534.x","article-title":"9p21 deletion in the diagnosis of malignant mesothelioma, using fluorescence in situ hybridization analysis","volume":"60","author":"Takeda","year":"2010","journal-title":"Pathol Int"},{"key":"2023012006334448400_b22-1410085","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1136\/jcp.2010.076794","article-title":"FISH assay development for the detection of p16\/CDKN2A deletion in malignant pleural mesothelioma","volume":"63","author":"Chung","year":"2010","journal-title":"J Clin Pathol"},{"key":"2023012006334448400_b23-1410085","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1007\/s00428-008-0689-3","article-title":"Prognostic significance of p16\/cdkn2a loss in pleural malignant mesotheliomas","volume":"453","author":"Dacic","year":"2008","journal-title":"Virchows Arch"},{"key":"2023012006334448400_b24-1410085","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1378\/chest.09-1951","article-title":"Fluorescence in situ hybridization in the definitive diagnosis of malignant mesothelioma in effusion cytology","volume":"138","author":"Savic","year":"2010","journal-title":"Chest"},{"key":"2023012006334448400_b25-1410085","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1136\/jclinpath-2011-200208","article-title":"Genomic gains and losses in malignant mesothelioma demonstrated by FISH analysis of paraffin-embedded tissues","volume":"65","author":"Takeda","year":"2012","journal-title":"J Clin Pathol"}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/141\/1\/85\/48795453\/ajcp_141_1_85.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/141\/1\/85\/48795453\/ajcp_141_1_85.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T06:35:42Z","timestamp":1674196542000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/141\/1\/85\/1766538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,1]]},"references-count":25,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2014,1,1]]},"published-print":{"date-parts":[[2014,1,1]]}},"URL":"https:\/\/doi.org\/10.1309\/ajcp5knl7qtellyi","relation":{},"ISSN":["1943-7722","0002-9173"],"issn-type":[{"value":"1943-7722","type":"electronic"},{"value":"0002-9173","type":"print"}],"subject":[],"published-other":{"date-parts":[[2014,1,1]]},"published":{"date-parts":[[2014,1,1]]}}}