{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T11:01:49Z","timestamp":1781348509867,"version":"3.54.1"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives:<\/jats:title>\n                  <jats:p>Detection of BRAF V600E mutations in hairy cell leukemia (HCL) has important diagnostic utility. In this study, we sought to compare immunohistochemistry with an antibody specific for this mutation to a sensitive molecular assay.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods:<\/jats:title>\n                  <jats:p>The performance of the BRAF V600E\u2013specific VE1 antibody was compared with that of allele-specific polymerase chain reaction (PCR) in 22 formalin-fixed, paraffin-embedded (FFPE) specimens with HCL involvement, along with nine splenic marginal zone lymphomas (SMZLs), 10 follicular lymphomas (FLs), 10 mantle cell lymphomas (MCLs), and 10 chronic lymphocytic leukemia\/small lymphocytic lymphomas (CLL\/SLLs). An additional 11 SMZLs, 100 FLs, 20 MCLs, 83 CLL\/SLL specimens, and 49 reactive tonsils within tissue microarrays were stained with VE1.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results:<\/jats:title>\n                  <jats:p>A BRAF V600E mutation was detected in 17 (77.3%) of 22 HCL cases by PCR. Immunohistochemistry demonstrated VE1 staining in 20 (90.9%) cases, identifying low-level (~1%) involvement in three HCL cases that were mutation negative by PCR. Evaluation of additional material from these patients confirmed the presence of BRAF V600E. Thirty-nine non-HCL cases were negative by both methods. Within tissue microarrays, weak false-positive staining was observed in two (0.8%) of 263 non-HCL cases.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions:<\/jats:title>\n                  <jats:p>VE1 immunohistochemistry is more sensitive than allele-specific PCR in FFPE bone marrow specimens and can be applied to decalcified core biopsy specimens that are not appropriate for molecular techniques.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1309\/ajcpdn4q1jtfgcfc","type":"journal-article","created":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T18:15:46Z","timestamp":1418667346000},"page":"89-99","source":"Crossref","is-referenced-by-count":32,"title":["Evaluation of Allele-Specific PCR and Immunohistochemistry for the Detection of <i>BRAF<\/i> V600E Mutations in Hairy Cell Leukemia"],"prefix":"10.1093","volume":"143","author":[{"given":"Noah A.","family":"Brown","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Michigan, Ann Arbor."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan L.","family":"Betz","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan, Ann Arbor."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut C.","family":"Weigelin","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan, Ann Arbor."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kojo S. J.","family":"Elenitoba-Johnson","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan, Ann Arbor."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan S.","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan, Ann Arbor."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathanael G.","family":"Bailey","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan, Ann Arbor."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,1,1]]},"reference":[{"key":"2023012004193133000_b1-1430089","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1056\/NEJMoa1014209","article-title":"BRAF mutations in hairy-cell leukemia","volume":"364","author":"Tiacci","year":"2011","journal-title":"N Engl J Med"},{"key":"2023012004193133000_b2-1430089","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1182\/blood-2011-08-371179","article-title":"Simple genetic diagnosis of hairy cell leukemia by sensitive detection of the BRAF-V600E mutation","volume":"119","author":"Tiacci","year":"2012","journal-title":"Blood"},{"key":"2023012004193133000_b3-1430089","doi-asserted-by":"crossref","first-page":"780","DOI":"10.3324\/haematol.2011.054874","article-title":"Detection of BRAF mutations in patients with hairy cell leukemia and related lymphoproliferative disorders","volume":"97","author":"Blombery","year":"2012","journal-title":"Haematologica"},{"key":"2023012004193133000_b4-1430089","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1182\/blood-2011-08-368209","article-title":"The BRAF V600E mutation in hairy cell leukemia and other mature B-cell neoplasms","volume":"119","author":"Arcaini","year":"2012","journal-title":"Blood"},{"key":"2023012004193133000_b5-1430089","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1309\/AJCP5TWORA0TMXGL","article-title":"Investigation of the BRAF V600E mutation by pyrosequencing in lymphoproliferative disorders","volume":"138","author":"Laurini","year":"2012","journal-title":"Am J Clin Pathol"},{"key":"2023012004193133000_b6-1430089","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1309\/AJCPL0OPXI9LZITV","article-title":"Rapid detection and quantitation of BRAF mutations in hairy cell leukemia using a sensitive pyrosequencing assay","volume":"138","author":"Verma","year":"2012","journal-title":"Am J Clin Pathol"},{"key":"2023012004193133000_b7-1430089","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1111\/j.1751-553X.2012.01402.x","article-title":"Correlation of the BRAF V600E mutation in hairy cell leukaemia with morphology, cytochemistry and immunophenotype","volume":"34","author":"Langabeer","year":"2012","journal-title":"Int J Lab Hematol"},{"key":"2023012004193133000_b8-1430089","doi-asserted-by":"crossref","first-page":"3151","DOI":"10.1182\/blood-2011-10-383323","article-title":"Development and validation of a real-time quantification assay to detect and monitor BRAFV600E mutations in hairy cell leukemia","volume":"119","author":"Schnittger","year":"2012","journal-title":"Blood"},{"key":"2023012004193133000_b9-1430089","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/j.1365-2141.2011.08963.x","article-title":"Pyrosequencing of BRAF V600E in routine samples of hairy cell leukaemia identifies CD5+ variant hairy cell leukaemia that lacks V600E","volume":"157","author":"Lennerz","year":"2012","journal-title":"Br J Haematol"},{"key":"2023012004193133000_b10-1430089","doi-asserted-by":"crossref","first-page":"3330","DOI":"10.1182\/blood-2011-09-379339","article-title":"Both variant and IGHV4-34-expressing hairy cell leukemia lack the BRAF V600E mutation","volume":"119","author":"Xi","year":"2012","journal-title":"Blood"},{"key":"2023012004193133000_b11-1430089","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1097\/PAS.0b013e3182712481","article-title":"Phospho-ERK(THR202\/Tyr214) is overexpressed in hairy cell leukemia and is a useful diagnostic marker in bone marrow trephine sections","volume":"37","author":"Warden","year":"2013","journal-title":"Am J Surg Pathol"},{"key":"2023012004193133000_b12-1430089","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.leukres.2011.12.015","article-title":"Incidence of the BRAF V600E mutation in chronic lymphocytic leukaemia and prolymphocytic leukaemia","volume":"36","author":"Langabeer","year":"2012","journal-title":"Leuk Res"},{"key":"2023012004193133000_b13-1430089","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1097\/PAS.0b013e3182549b50","article-title":"Application of a BRAF V600E mutation-specific antibody for the diagnosis of hairy cell leukemia","volume":"36","author":"Andrulis","year":"2012","journal-title":"Am J Surg Pathol"},{"key":"2023012004193133000_b14-1430089","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1182\/blood-2013-07-513523","article-title":"BRAF V600E is also seen in unclassifiable splenic B-cell lymphoma\/leukemia, a potential mimic of hairy cell leukemia","volume":"122","author":"Raess","year":"2013","journal-title":"Blood"},{"key":"2023012004193133000_b15-1430089","article-title":"Requisite analytic and diagnostic performance characteristics for the clinical detection of BRAF V600E in hairy cell leukemia: a comparison of 2 allele-specific PCR assays [published online February 5, 2014]","author":"Brown","journal-title":"Appl Immunohistochem Mol Morphol"},{"key":"2023012004193133000_b16-1430089","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s00401-011-0887-y","article-title":"Immunohistochemical testing of BRAF V600E status in 1,120 tumor tissue samples of patients with brain metastases","volume":"123","author":"Capper","year":"2012","journal-title":"Acta Neuropathol"},{"key":"2023012004193133000_b17-1430089","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s00401-011-0841-z","article-title":"Assessment of BRAF V600E mutation status by immunohistochemistry with a mutation-specific monoclonal antibody","volume":"122","author":"Capper","year":"2011","journal-title":"Acta Neuropathol"},{"key":"2023012004193133000_b18-1430089","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1097\/PAS.0b013e318246b527","article-title":"Immunohistochemical detection of the BRAF V600E\u2013mutated protein in papillary thyroid carcinoma","volume":"36","author":"Koperek","year":"2012","journal-title":"Am J Surg Pathol"},{"key":"2023012004193133000_b19-1430089","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1002\/gcc.22070","article-title":"BRAF V600E mutation detection by immunohistochemistry in colorectal carcinoma","volume":"52","author":"Affolter","year":"2013","journal-title":"Genes Chromosomes Cancer"},{"key":"2023012004193133000_b20-1430089","doi-asserted-by":"crossref","first-page":"2765","DOI":"10.1002\/cncr.28133","article-title":"Mutation-specific antibody detects mutant BRAF(V600E) protein expression in human colon carcinomas","volume":"119","author":"Sinicrope","year":"2013","journal-title":"Cancer"},{"key":"2023012004193133000_b21-1430089","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/his.12154","article-title":"Immunohistochemistry using the BRAF V600E mutation-specific monoclonal antibody VE1 is not a useful surrogate for genotyping in colorectal adenocarcinoma","volume":"63","author":"Adackapara","year":"2013","journal-title":"Histopathology"},{"key":"2023012004193133000_b22-1430089","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/modpathol.2013.126","article-title":"Improved molecular classification of serrated lesions of the colon by immunohistochemical detection of BRAF V600E","volume":"27","author":"Mesteri","year":"2014","journal-title":"Mod Pathol"},{"key":"2023012004193133000_b23-1430089","volume-title":"WHO Classification of Tumours of Hematopoietic and Lymphoid Tissues","author":"Swerdlow","year":"2008"},{"key":"2023012004193133000_b24-1430089","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1002\/cncy.20180","article-title":"Application of immunocytochemistry and BRAF mutational analysis to direct smears of metastatic melanoma","volume":"120","author":"Hookim","year":"2012","journal-title":"Cancer Cytopathol"},{"key":"2023012004193133000_b25-1430089","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2307\/2529310","article-title":"The measurement of observer agreement for categorical data","volume":"33","author":"Landis","year":"1977","journal-title":"Biometrics"}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/143\/1\/89\/48790994\/ajcp_143_1_89.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/143\/1\/89\/48790994\/ajcp_143_1_89.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T04:20:13Z","timestamp":1674188413000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/143\/1\/89\/1761445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,1]]},"references-count":25,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2015,1,1]]},"published-print":{"date-parts":[[2015,1,1]]}},"URL":"https:\/\/doi.org\/10.1309\/ajcpdn4q1jtfgcfc","relation":{},"ISSN":["1943-7722","0002-9173"],"issn-type":[{"value":"1943-7722","type":"electronic"},{"value":"0002-9173","type":"print"}],"subject":[],"published-other":{"date-parts":[[2015,1,1]]},"published":{"date-parts":[[2015,1,1]]}}}