{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T02:17:52Z","timestamp":1772763472656,"version":"3.50.1"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The progression of cervical intraepithelial lesions to invasive cancer is associated with corresponding reductions in human papillomavirus (HPV) L1 capsid antigen (L1) expression. We sought to determine whether a similar loss of L1 occurs during anal carcinogenesis using immunohistochemistry on paraffin-embedded sections as well as INNO-LiPA HPV Genotyping (Innogenetics, Gent, Belgium) technology to determine HPV infection status. We analyzed 31 squamous cell carcinomas (SCCs), 26 SCCs in situ (SCC-IS), and 11 normal anal mucosae from 36 patients. High-risk HPV subtypes were detected in all patients. L1 nuclear staining was identified in 38% of SCC-IS; however, there was no detection in normal anal mucosae, SCC, or recurrent SCC. Of those SCC-IS associated with a concomitant invasive SCC, only 15% demonstrated nuclear L1 expression as compared to 62% of isolated SCC-IS (P = .02). Nuclear expression of L1 is lost in the progression of anal SCC-IS to SCC and may serve as a possible prognostic marker of enhanced malignant potential.<\/jats:p>","DOI":"10.1309\/ajcpr5vd6nsqrwbn","type":"journal-article","created":{"date-parts":[[2011,2,24]],"date-time":"2011-02-24T18:37:47Z","timestamp":1298572667000},"page":"436-441","source":"Crossref","is-referenced-by-count":15,"title":["HPV L1 Capsid Protein Detection and Progression of Anal Squamous Neoplasia"],"prefix":"10.1093","volume":"135","author":[{"given":"Jonathan","family":"Hernandez","sequence":"first","affiliation":[{"name":"1Gastrointestinal Oncology, Tampa, FL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abul","family":"Elahi","sequence":"additional","affiliation":[{"name":"1Gastrointestinal Oncology, Tampa, FL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erin","family":"Siegel","sequence":"additional","affiliation":[{"name":"2Risk Assessment, Detection and Intervention, Tampa, FL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Domenico","family":"Coppola","sequence":"additional","affiliation":[{"name":"3Anatomic Pathology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bridgett","family":"Riggs","sequence":"additional","affiliation":[{"name":"2Risk Assessment, Detection and Intervention, Tampa, FL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Shibata","sequence":"additional","affiliation":[{"name":"1Gastrointestinal Oncology, Tampa, FL"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2011,3,1]]},"reference":[{"key":"2023011909493294100_b1-1350436","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s00705-009-0310-3","article-title":"Insights into the role and function of L2, the minor capsid protein of papillomaviruses","volume":"154","author":"Pereira","year":"2009","journal-title":"Arch Virol"},{"key":"2023011909493294100_b2-1350436","first-page":"1934","article-title":"Human papillomavirus 16 and 18 L1 serology compared 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