{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T21:09:37Z","timestamp":1792271377290,"version":"4.4.0"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The histopathologic interpretation of cervical intraepithelial neoplasia (CIN) is subject to a high level of interobserver variability and a substantial number of false-positive and false-negative results. We assessed the impact of the conjunctive interpretation of p16INK4a-immunostained slides on the accuracy of community-based pathologists in diagnosing high-grade cervical intraepithelial neoplasia (CIN; CIN 2 and CIN 3) in biopsy specimens. Twelve pathologists rendered independent diagnoses on a set of 500 H&amp;E-stained cervical punch and conization specimens. Results were compared with a dichotomized \u201cgold standard\u201d established by consensus of 3 gynecopathology experts. When p16INK4a-immunostained slides were added and conjunctively interpreted with the H&amp;E-stained slides, a significant increase in diagnostic accuracy for the detection of high-grade CIN was observed (P = .0004). Sensitivity for high-grade CIN was increased by 13%, cutting the rate of false-negative results in half. Agreement of community-based pathologists in diagnosing high-grade CIN was significantly improved (mean \u03ba values advanced from 0.566 to 0.749; P &amp;lt; .0001). Reproducibility of p16INK4a stain interpretation was excellent (\u03ba = 0.899). Our results show that conjunctive interpretation of p16INK4a-stained slides could significantly improve the routine interpretation of cervical histopathology.<\/jats:p>","DOI":"10.1309\/ajcpxsvcdz3d5mzm","type":"journal-article","created":{"date-parts":[[2010,2,12]],"date-time":"2010-02-12T21:03:15Z","timestamp":1266008595000},"page":"395-406","source":"Crossref","is-referenced-by-count":191,"title":["Conjunctive p16INK4a Testing Significantly Increases Accuracy in Diagnosing High-Grade Cervical Intraepithelial Neoplasia"],"prefix":"10.1093","volume":"133","author":[{"given":"Christine","family":"Bergeron","sequence":"first","affiliation":[{"name":"1Laboratoire Cerba, Cergy Pontoise, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaume","family":"Ordi","sequence":"additional","affiliation":[{"name":"2Cl\u00ednic Hospital Universitari, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietmar","family":"Schmidt","sequence":"additional","affiliation":[{"name":"3Institute of Pathology, Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus J.","family":"Trunk","sequence":"additional","affiliation":[{"name":"4mtm laboratories, Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Keller","sequence":"additional","affiliation":[{"name":"5Acomed statistik, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruediger","family":"Ridder","sequence":"additional","affiliation":[{"name":"4mtm laboratories, Heidelberg, 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