{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T15:28:50Z","timestamp":1783006130114,"version":"3.54.5"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,2,1]]},"abstract":"Abstract<\/jats:title>\n \n Objectives:<\/jats:title>\n To determine the clinical utility and performance characteristics of a laboratory-adapted flow cytometric method for the detection of acetylcholine receptor (AChR) modulating antibodies in myasthenia gravis (MG).<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n Serum samples from 120 healthy donors and 100 patients with suspected MG were assessed for the ability to reduce surface AChR concentrations (antigenic modulation) in RD (TE671) or DB40 human muscle cell lines by flow cytometry. Reference ranges were established by receiver operating characteristic curve analysis, and results were then compared with those of the current radioimmunoassay (RIA).<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n Flow cytometric results from the RD cell line had an interpretive threshold of 46% modulation or greater and correlated best (98% sensitivity, 99% specificity) with those of the current RIA.<\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n The new flow cytometric method using the RD cell platform provided higher quality clinical results, a more robust and efficient assay format, a significant cost savings, and less environmental burden.<\/jats:p>\n <\/jats:sec>","DOI":"10.1309\/ajcpyeor6sge8zlu","type":"journal-article","created":{"date-parts":[[2015,1,16]],"date-time":"2015-01-16T18:35:55Z","timestamp":1421433355000},"page":"186-192","source":"Crossref","is-referenced-by-count":26,"title":["Detection of Acetylcholine Receptor Modulating Antibodies by Flow Cytometry"],"prefix":"10.1093","volume":"143","author":[{"given":"Bucky K.","family":"Lozier","sequence":"first","affiliation":[{"name":"1Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R.","family":"Haven","sequence":"additional","affiliation":[{"name":"1Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT"},{"name":"2ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark E.","family":"Astill","sequence":"additional","affiliation":[{"name":"2ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry R.","family":"Hill","sequence":"additional","affiliation":[{"name":"1Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT"},{"name":"2ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT"},{"name":"3Department of Pediatrics, University of Utah School of Medicine, Salt Lake City"},{"name":"4Department of Medicine, University of Utah School of Medicine, Salt Lake City."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,2,1]]},"reference":[{"key":"2023012004412498100_b1-1430186","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/978-1-59745-156-7_3","article-title":"Immunopathogenesis of myasthenia gravis","volume-title":"Myasthenia Gravis and Related Disorders","author":"Conti-Fine","year":"2009","edition":"2nd"},{"key":"2023012004412498100_b2-1430186","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/1750-1172-2-44","article-title":"Myasthenia gravis","volume":"2","author":"Juel","year":"2007","journal-title":"Orphanet J Rare Dis"},{"key":"2023012004412498100_b3-1430186","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/978-1-59745-156-7_5","article-title":"Clinical presentation and epidemiology of myasthenia gravis","volume-title":"Myasthenia Gravis and Related Disorders","author":"Kuks","year":"2009","edition":"2nd"},{"key":"2023012004412498100_b4-1430186","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1111\/j.1749-6632.1981.tb33765.x","article-title":"Aspects of the natural history of myasthenia gravis: crisis and death","volume":"377","author":"Cohen","year":"1981","journal-title":"Ann N Y Acad Sci"},{"key":"2023012004412498100_b5-1430186","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1111\/j.1468-1331.2010.03019.x","article-title":"Guidelines for treatment of autoimmune neuromuscular transmission disorders","volume":"17","author":"Skeie","year":"2010","journal-title":"Eur J Neurol"},{"key":"2023012004412498100_b6-1430186","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/S1474-4422(09)70063-8","article-title":"Autoimmune myasthenia gravis: emerging clinical and biological heterogeneity","volume":"8","author":"Merrigioli","year":"2009","journal-title":"Lancet Neurol"},{"key":"2023012004412498100_b7-1430186","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1111\/j.1749-6632.1987.tb51321.x","article-title":"Clinical correlations of antibodies that bind, block, or modulate human acetylcholine receptors in myasthenia gravis","volume":"505","author":"Howard","year":"1987","journal-title":"Ann N Y Acad Sci"},{"key":"2023012004412498100_b8-1430186","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1212\/WNL.47.6.1552","article-title":"A transfected human muscle cell line expressing the adult subtype of the human muscle acetylcholine receptor for diagnostic assays in myasthenia gravis","volume":"47","author":"Beeson","year":"1996","journal-title":"Neurology"},{"key":"2023012004412498100_b9-1430186","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1002\/(SICI)1098-2825(1998)12:5<315::AID-JCLA12>3.0.CO;2-Y","article-title":"Development of an assay for modulating anti-acetylcholine receptor autoantibodies using human rhabdomyosarcoma cell line","volume":"12","author":"Lyons","year":"1998","journal-title":"J Clin Lab Anal"},{"key":"2023012004412498100_b10-1430186","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1002\/cyto.b.20454","article-title":"A rapid, fluorescence-based assay for detecting antigenic modulation of the acetylcholine receptor on human cell lines","volume":"76","author":"Keefe","year":"2009","journal-title":"Cytometry B Clin Cytom"},{"key":"2023012004412498100_b11-1430186","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1373\/clinchem.2009.140392","article-title":"An algorithm for acetylcholine receptor antibody testing in patients with suspected myasthenia gravis","volume":"56","author":"Haven","year":"2010","journal-title":"Clin Chem"}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/143\/2\/186\/48791155\/ajcp_143_2_186.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/143\/2\/186\/48791155\/ajcp_143_2_186.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T04:42:59Z","timestamp":1674189779000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/143\/2\/186\/1766603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,1]]},"references-count":11,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,2,1]]},"published-print":{"date-parts":[[2015,2,1]]}},"URL":"https:\/\/doi.org\/10.1309\/ajcpyeor6sge8zlu","relation":{},"ISSN":["1943-7722","0002-9173"],"issn-type":[{"value":"1943-7722","type":"electronic"},{"value":"0002-9173","type":"print"}],"subject":[],"published-other":{"date-parts":[[2015,2,1]]},"published":{"date-parts":[[2015,2,1]]}}}