{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T14:46:24Z","timestamp":1773153984783,"version":"3.50.1"},"reference-count":29,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2001,3,1]],"date-time":"2001-03-01T00:00:00Z","timestamp":983404800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:p> Quinolones and magnesium deficiency cause similar lesions in joint cartilage of young animals. Chondrocytes cultivated in the presence of quinolones and in Mg-free medium show severe alterations in cytoskeleton and decreased ability to adhere to the culture dish. We investigated whether Mg<jats:sup>2+<\/jats:sup> supplementation can prevent quinolone-mediated effects on chondrocytes in vitro. Chondrocytes cultivated in Dulbecco's modified Eagle's medium\/HAM's F-12 medium were treated with ciprofloxacin (80 and 160 \u03bcg\/ml) and enrofloxacin (100 and 150 \u03bcg\/ml). Mg<jats:sup>2+<\/jats:sup> was added at a concentration of 0.0612 mg\/ml (MgCl) and 0.0488 mg\/ml (MgSO<jats:sub>4<\/jats:sub>) or a triple dose. In addition, cells were cultivated in Mg-free medium and accordingly treated with Mg<jats:sup>2+<\/jats:sup> supplementation. After 5 days in culture, the number of adherent cells per milliliter was determined. The number of chondrocytes in quinolone-treated groups decreased to 12-36% that of the control group within the culture period. With Mg<jats:sup>2+<\/jats:sup> supplementation, the number of attached cells increased to 40-70% that of control cells. The threefold dose of Mg<jats:sup>2+<\/jats:sup> led to better results than did the single dose. Cell proliferation tested by immunohistochemical staining with Ki67 (clone MIB5) decreased from 70% in control groups to 55%, 48%, and 30% in enrofloxacintreated groups in a concentration dependent manner (50, 100, and 150 \u03bcg\/ml). Addition of Mg<jats:sup>2+<\/jats:sup> did not increase the rate of cell proliferation. These results suggest that a great part of quinolone-induced damage is due to magnesium complex formation, as Mg<jats:sup>2+<\/jats:sup> supplementation is able to reduce the effects in vitro. However, quinolone effects on cell proliferation seem to be an independent process that is not influenced by magnesium supplementation. <\/jats:p>","DOI":"10.1354\/vp.38-2-143","type":"journal-article","created":{"date-parts":[[2003,4,24]],"date-time":"2003-04-24T20:17:42Z","timestamp":1051215462000},"page":"143-148","source":"Crossref","is-referenced-by-count":25,"title":["In Vitro Evidence for Effects of Magnesium Supplementation on Quinolone-treated Horse and Dog Chondrocytes"],"prefix":"10.1177","volume":"38","author":[{"given":"M.","family":"Egerbacher","sequence":"first","affiliation":[{"name":"Institute of Histology and Embryology, University of Veterinary Medicine, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Wolfesberger","sequence":"additional","affiliation":[{"name":"Institute of Histology and Embryology, University of Veterinary Medicine, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Gabler","sequence":"additional","affiliation":[{"name":"Institute of Nutrition, University of Veterinary Medicine, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2001,3,1]]},"reference":[{"key":"bibr1-vp.38-2-143","first-page":"2868","volume":"4","author":"Albelda SM","year":"1990","journal-title":"Fed Am Soc Exp Biol J"},{"key":"bibr2-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380919"},{"key":"bibr3-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1177\/030098589002700303"},{"key":"bibr4-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1006\/faat.1993.1034"},{"key":"bibr5-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1177\/030098589202900307"},{"key":"bibr6-vp.38-2-143","unstructured":"6 Defilippi P, Gismondi A, Santoni A, Tarone G: Signal Transduction by integrins, pp. 143\u2013155. Landes Biosciences, Austin, TX, 1997"},{"key":"bibr7-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.12.7661"},{"key":"bibr8-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1993.1189"},{"key":"bibr9-vp.38-2-143","unstructured":"9 Egerbacher M, Edinger J, Tschulenk W: Effects of enrofloxacin and ciprofloxacin on dog and horse cultured chondrocytes. Am J Vet Res (in press)"},{"key":"bibr10-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1007\/s002040050008"},{"key":"bibr11-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-9335(99)80081-8"},{"key":"bibr12-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1007\/s002040050272"},{"issue":"13","key":"bibr13-vp.38-2-143","first-page":"6","volume":"5","author":"F\u00f6rster C","year":"1996","journal-title":"Chemother J"},{"key":"bibr14-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.2.243"},{"key":"bibr15-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1007\/BF01977402"},{"key":"bibr16-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90115-S"},{"key":"bibr17-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.9.1979"},{"key":"bibr18-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.12.2810"},{"key":"bibr19-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1006\/faat.1995.1146"},{"key":"bibr20-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780360811"},{"key":"bibr21-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1994.1053"},{"key":"bibr22-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5173(93)90170-K"},{"key":"bibr23-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.9.2013"},{"issue":"3","key":"bibr24-vp.38-2-143","first-page":"148","volume":"5","author":"Stahlmann R","year":"1999","journal-title":"Clin Microbiol Inf"},{"key":"bibr25-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1007\/BF02010725"},{"key":"bibr26-vp.38-2-143","first-page":"7","volume":"19","author":"Stahlmann R","year":"1997","journal-title":"Magnesium Bull"},{"key":"bibr27-vp.38-2-143","first-page":"1544","volume":"271","author":"Thuong-Guyot M","year":"1994","journal-title":"J Pharmacol Exp Ther"},{"key":"bibr28-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1007\/s002239900328"},{"key":"bibr29-vp.38-2-143","doi-asserted-by":"publisher","DOI":"10.1002\/sca.1998.4950200705"}],"container-title":["Veterinary Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1354\/vp.38-2-143","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1354\/vp.38-2-143","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1354\/vp.38-2-143","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T10:16:22Z","timestamp":1740996982000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1354\/vp.38-2-143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,3]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,3]]}},"alternative-id":["10.1354\/vp.38-2-143"],"URL":"https:\/\/doi.org\/10.1354\/vp.38-2-143","relation":{},"ISSN":["0300-9858","1544-2217"],"issn-type":[{"value":"0300-9858","type":"print"},{"value":"1544-2217","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,3]]}}}