{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T13:49:20Z","timestamp":1775742560100,"version":"3.50.1"},"reference-count":45,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2004,7,1]],"date-time":"2004-07-01T00:00:00Z","timestamp":1088640000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:p> Cyclooxygenase-2 (COX-2) and nitric oxide synthase 2 (NOS2) were detected and localized in 20 pigs with ulcerative colitis caused by natural infection with Salmonella typhimurium. Evidence of NOS2 activity was determined by the formation of nitrotyrosine, a reaction product of peroxynitrite, in NOS2-expressing ulcerative colons by immunohistochemistry. Transcript RNA of COX-2 and NOS2 was consistently detected in colonic tissues from the 20 pigs with ulcerative colitis by using reverse transcription-polymerase chain reaction. Immunohistochemical signals for COX-2 and NOS2 were detected in the ulcerated area of all 20 pigs. Expression of COX-2 and NOS2 was identified continuously within inflammatory intestinal lesions but was minimal in unaffected regions of the colon of S. typhimurium\u2014infected pigs. The immunohistochemistry of serial sections of intestine indicated that the majority of colons containing numerous COX-2\u2014positive cells also had numerous NOS2-positive cells. Localization of NOS2 and a nitrotyrosine antigen was prominent in neutrophils and macrophages in the periphery of the lesions. Simultaneous detection of COX-2 and NOS2 RNA and protein indicated functional activity of prostaglandin and NO production in vivo. This study suggested that COX-2 and NOS2 expression may play a role in the pathophysiologic processes in ulcerative colitis caused by S. typhimurium. <\/jats:p>","DOI":"10.1354\/vp.41-4-419","type":"journal-article","created":{"date-parts":[[2004,7,1]],"date-time":"2004-07-01T20:55:42Z","timestamp":1088715342000},"page":"419-423","source":"Crossref","is-referenced-by-count":14,"title":["Expression of Cyclooxygenase-2 and Nitric Oxide Synthase 2 in Swine Ulcerative Colitis Caused by <i>Salmonella 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