{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:08:57Z","timestamp":1789682937088,"version":"4.0.1"},"reference-count":32,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2005,7,1]],"date-time":"2005-07-01T00:00:00Z","timestamp":1120176000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:p>We examined the expression of CD20 in normal canine peripheral blood mononuclear cells, normal canine spleen, and canine non-Hodgkin lymphoma (NHL) to determine the feasibility of using this antigen as a diagnostic aid and as a possible target for therapy. An antibody generated against a C-terminal (intracytoplasmic) epitope of human CD20 recognized proteins of 32-36 kd in normal and malignant canine lymphocytes. This antibody showed restricted membrane binding in a subset of lymphocytes in peripheral blood, in the B-cell regions from a normal canine spleen and lymph node, and in malignant cells from 19 dogs with B-cell NHL, but not from 15 dogs with T-cell NHL. The patterns of CD20 reactivity in these samples overlapped those seen using an antibody that recognizes canine CD79a. This anti-CD20 antibody is therefore suitable as an aid to phenotype canine NHL. In contrast, normal canine B cells were not recognized by any of 28 antibodies directed against the extracellular domains of human CD20 (including the chimeric mouse-human antibody Rituximab) or by any of 12 antibodies directed against the extracellular domains of mouse CD20. Thus, the use of CD20 as a therapeutic target will require the generation of specific antibodies against the extracellular domains of canine CD20.<\/jats:p>","DOI":"10.1354\/vp.42-4-468","type":"journal-article","created":{"date-parts":[[2005,7,8]],"date-time":"2005-07-08T18:56:42Z","timestamp":1120849002000},"page":"468-476","source":"Crossref","is-referenced-by-count":79,"title":["CD20 Expression in Normal Canine B Cells and in Canine non-Hodgkin Lymphoma"],"prefix":"10.1177","volume":"42","author":[{"given":"C. M.","family":"Jubala","sequence":"first","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado Health Sciences Center, Denver, CO"},{"name":"AMC Cancer Center, University of Colorado Health Sciences Center, Denver, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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