{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T23:04:06Z","timestamp":1778886246474,"version":"3.51.4"},"reference-count":23,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:p> Serial sections of formalin-fixed, paraffin-embedded muscle biopsy specimens from 28 Quarter Horse, Paint, and draft-related breeds, aged 0.5-23 years, were treated with periodic acid-Schiff (PAS) stain for glycogen and were immunostained to detect ubiquitin expression. On the basis of findings in PAS-stained sections, a diagnosis of equine polysaccharide storage myopathy (EPSSM) was made in 22 horses aged 2-23 years (mean, 9.4 years); samples from 6 horses aged 0.5-15 years (mean, 7.3 years) had a normal PAS staining pattern, with no relevant lesions. Ubiquitin expression was detected in all but a 2-year-old EPSSM-affected horse and was not detected in the non-EPSSM-affected horses. Ubiquitin expression was greater than the degree of PAS-positive, amylase-resistant material, and ubiquitin was detected in aggregates of amylase-sensitive glycogen as well as in aggregates of amylase-resistant material. Results suggest that glycogen aggregates develop and are ubiquitinated prior to development of amylase-resistant inclusions. Ubiquitin immunostaining may be most useful for confirming the diagnosis of EPSSM in horses with only amylase-sensitive glycogen aggregates and in horses with early amylase-resistant inclusions. However, ubiquitin immunostaining is no more sensitive than is PAS staining for diagnosis of EPSSM. <\/jats:p>","DOI":"10.1354\/vp.43-3-270","type":"journal-article","created":{"date-parts":[[2006,5,3]],"date-time":"2006-05-03T19:14:13Z","timestamp":1146683653000},"page":"270-275","source":"Crossref","is-referenced-by-count":15,"title":["Ubiquitin Expression in Muscle from Horses with Polysaccharide Storage Myopathy"],"prefix":"10.1177","volume":"43","author":[{"given":"B. A.","family":"Valentine","sequence":"first","affiliation":[{"name":"Veterinary Diagnostic Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. H.","family":"Flint","sequence":"additional","affiliation":[{"name":"Veterinary Diagnostic Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. A.","family":"Fischer","sequence":"additional","affiliation":[{"name":"Veterinary Diagnostic Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2006,5,1]]},"reference":[{"key":"bibr1-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.12.7672505"},{"key":"bibr2-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(98)01276-4"},{"key":"bibr3-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2005.66.839"},{"key":"bibr4-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1136\/vr.144.3.73"},{"key":"bibr5-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1007\/BF00227716"},{"key":"bibr6-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1892\/0891-6640(2002)016<0581:DOOPSM>2.3.CO;2"},{"key":"bibr7-vp.43-3-270","doi-asserted-by":"crossref","first-page":"458","DOI":"10.2460\/ajvr.1999.60.04.458","volume":"60","author":"De La Corte FD","year":"1999","journal-title":"Am J Vet Res"},{"key":"bibr8-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1007\/s004010050972"},{"key":"bibr9-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1007\/s004010050971"},{"key":"bibr10-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199201000-00004"},{"key":"bibr11-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1016\/j.cccn.2003.10.025"},{"key":"bibr12-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1177\/030098589603300317"},{"key":"bibr13-vp.43-3-270","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1002\/path.1711550105","volume":"155","author":"Lowe J","year":"1988","journal-title":"J Pathol"},{"key":"bibr14-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.1993.tb00726.x"},{"key":"bibr15-vp.43-3-270","first-page":"505","volume":"134","author":"Manetto V","year":"1989","journal-title":"Am J Pathol"},{"key":"bibr16-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.2746\/042516402776767259"},{"key":"bibr17-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-8966(06)80006-4"},{"key":"bibr18-vp.43-3-270","doi-asserted-by":"crossref","first-page":"782","DOI":"10.2460\/ajvr.1998.59.06.782","volume":"59","author":"Valberg SJ","year":"1998","journal-title":"Am J Vet Res"},{"key":"bibr19-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1354\/vp.42-6-823"},{"key":"bibr20-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1997.tb01672.x"},{"key":"bibr21-vp.43-3-270","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.2460\/javma.1999.215.11.1661","volume":"215","author":"Valentine BA","journal-title":"J Am Vet Med Assoc"},{"key":"bibr22-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3292.1998.tb00846.x"},{"key":"bibr23-vp.43-3-270","doi-asserted-by":"publisher","DOI":"10.1177\/104063870101300112"}],"container-title":["Veterinary Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1354\/vp.43-3-270","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1354\/vp.43-3-270","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1354\/vp.43-3-270","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T16:01:35Z","timestamp":1741017695000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1354\/vp.43-3-270"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1354\/vp.43-3-270"],"URL":"https:\/\/doi.org\/10.1354\/vp.43-3-270","relation":{},"ISSN":["0300-9858","1544-2217"],"issn-type":[{"value":"0300-9858","type":"print"},{"value":"1544-2217","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5]]}}}