{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T16:20:50Z","timestamp":1771518050521,"version":"3.50.1"},"reference-count":10,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:p> Whereas the adrenal glands of healthy ferrets produce only limited amounts of androgenic steroids, adrenocortical neoplasms that arise in neutered ferrets typically secrete androgens or their derivative, estrogen. The 17,20-lyase activity of cytochrome P450 17\u03b1-hydroxylase\/17,20-lyase (P450c17) must increase to permit androgen biosynthesis in neoplastic adrenal tissue. We screened ferret adrenocortical tumor specimens for expression of cytochrome b<jats:sub>5<\/jats:sub> (cyt b<jats:sub>5<\/jats:sub>), an allosteric regulator that selectively enhances the 17,20-lyase activity of P450c17. Cyt b<jats:sub>5<\/jats:sub> immunoreactivity was evident in 24 of 25 (96%) adrenocortical adenomas\/carcinomas from ferrets with signs of ectopic sex steroid production. Normal adrenocortical cells lacked cyt b<jats:sub>5<\/jats:sub>, which may account for the low production of adrenal androgens in healthy ferrets. Other markers characteristic of gonadal somatic cells, such as luteinizing hormone receptor, aromatase, and GATA4, were coexpressed with cyt b<jats:sub>5<\/jats:sub> in some of the tumors. We concluded that cyt b<jats:sub>5<\/jats:sub> is upregulated during gonadectomy-induced adrenocortical neoplasia and is a marker of androgen synthetic potential in these tumors. <\/jats:p>","DOI":"10.1354\/vp.45-4-439","type":"journal-article","created":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:50:56Z","timestamp":1214614256000},"page":"439-442","source":"Crossref","is-referenced-by-count":20,"title":["Cytochrome <i>b<\/i><sub>5<\/sub> Expression in Gonadectomy-induced Adrenocortical Neoplasms of the Domestic Ferret (<i>Mustela putorius furo<\/i>)"],"prefix":"10.1177","volume":"45","author":[{"given":"S.","family":"Wagner","sequence":"first","affiliation":[{"name":"Departments of Pediatrics and Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO"},{"name":"University of Applied Sciences Mannheim, Mannheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kiupel","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation, Michigan State University College of Veterinary Medicine, Lansing, MI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"II","given":"R. A.","family":"Peterson","sequence":"additional","affiliation":[{"name":"GlaxoSmithKline, Research Triangle Park, NC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Heikinheimo","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO"},{"name":"Children's Hospital, Institute of Biomedicine, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. B.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2008,7,1]]},"reference":[{"key":"bibr1-vp.45-4-439","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.06375"},{"key":"bibr2-vp.45-4-439","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-861545"},{"key":"bibr3-vp.45-4-439","doi-asserted-by":"publisher","DOI":"10.1354\/vp.43-2-97"},{"key":"bibr4-vp.45-4-439","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0990361"},{"key":"bibr5-vp.45-4-439","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2006.12.023"},{"key":"bibr6-vp.45-4-439","doi-asserted-by":"publisher","DOI":"10.1354\/vp.40-2-136"},{"key":"bibr7-vp.45-4-439","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.2460\/javma.1996.209.06.1097","volume":"209","author":"Rosenthal KL","year":"1996","journal-title":"J Am Vet Med Assoc"},{"key":"bibr8-vp.45-4-439","first-page":"36","volume":"78","author":"Sakai Y","year":"1994","journal-title":"J Clin Endocrinol Metab"},{"key":"bibr9-vp.45-4-439","first-page":"1286","volume":"76","author":"Sakai Y","year":"1993","journal-title":"J Clin Endocrinol Metab"},{"key":"bibr10-vp.45-4-439","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.23.10619"}],"container-title":["Veterinary Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1354\/vp.45-4-439","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1354\/vp.45-4-439","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1354\/vp.45-4-439","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T22:29:02Z","timestamp":1740868142000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1354\/vp.45-4-439"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7]]},"references-count":10,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,7]]}},"alternative-id":["10.1354\/vp.45-4-439"],"URL":"https:\/\/doi.org\/10.1354\/vp.45-4-439","relation":{},"ISSN":["0300-9858","1544-2217"],"issn-type":[{"value":"0300-9858","type":"print"},{"value":"1544-2217","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7]]}}}