{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T16:57:25Z","timestamp":1773248245425,"version":"3.50.1"},"reference-count":37,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:p> Several immunohistochemical markers have been used to demonstrate the presence of myoepithelial cells in order to determine their role in the histogenesis of mammary tumors. p63, a recently characterized p53 homologue, is consistently expressed in myoepithelial cells of the human breast; however, no assessment of its immunoreactivity has been reported so far in canine mammary tissues. We investigated p63 immunohistochemical expression, as a novel myoepithelial cell nuclear marker, in 81 samples of normal ( n = 2), hyperplastic ( n = 11), and neoplastic ( n = 68) canine mammary tissues. Myoepithelial phenotype was confirmed by using complementary monoclonal antibodies: alpha-smooth muscle actin, cytokeratin 14, cytokeratin AE1\/AE3, and vimentin. p63 expression was observed in 91.4% (74\/81) of the samples evaluated. Normal mammary glands, mammary hyperplasias, and benign tumors showed 100% immunoreactivity, with p63 expression restricted to myoepithelial cell nuclei. In general, benign mixed tumors showed a basal cell compartment immunoreactive to p63, with a gradual decrease of its expression during myoepithelial transformation. p63 expression was found in 72% of malignant tumors, allowing myoepithelial or basal cell identification in spindle-cell carcinomas (2\/2), tubulopapillary carcinomas (8\/9), solid carcinomas (7\/10), and carcinosarcomas (1\/3). The osteosarcoma analyzed was p63 negative. In our series, stromal components were consistently nonreactive to p63. In conclusion, the present study reveals p63 as a sensitive and highly specific marker of myoepithelial cells in canine mammary tissues, and the authors suggest p63 as an additional marker for defining myoepithelial histogenesis. <\/jats:p>","DOI":"10.1354\/vp.40-4-412","type":"journal-article","created":{"date-parts":[[2003,6,24]],"date-time":"2003-06-24T20:47:32Z","timestamp":1056487652000},"page":"412-420","source":"Crossref","is-referenced-by-count":80,"title":["p63: A Novel Myoepithelial Cell Marker in Canine Mammary Tissues"],"prefix":"10.1177","volume":"40","author":[{"given":"A.","family":"Gama","sequence":"first","affiliation":[{"name":"Department of Pathology and Veterinary Clinics of the University of Tr\u00e1s os Montes e Alto Douro, Vila Real, Portugal"}]},{"given":"A.","family":"Alves","sequence":"additional","affiliation":[{"name":"Department of Pathology and Veterinary Clinics of the University of Tr\u00e1s os Montes e Alto Douro, Vila Real, Portugal"}]},{"given":"F.","family":"Gartner","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, University of Porto, Porto, Portugal"}]},{"given":"F.","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal"},{"name":"Medical Faculty, University of Porto, Porto, 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