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To obtain a better insight into the genesis and neoplastic transformation process of bovine OSCC, abnormal protein expression and proliferation index were assessed by the immunohistochemical evaluation of p53 and Ki67, respectively. OSCC samples were collected from 15 bovines and were classified histologically according to the degree of differentiation into three categories: poorly, moderately, and well differentiated. Immunohis-tochemistry using polyclonal anti-human p53 antibody and polyclonal anti-human Ki67 antibody was performed. Ten of 15 tumors tested were immunoreactive for p53. Twelve tumors demonstrated Ki67 expression. As in human squamous cell carcinoma, p53 overexpression is frequent in bovine OSCC, providing support for a possible role of the protein in the pathogenesis of this neoplasia. No correlation between the percentage of p53 stained nuclei and the degree of differentiation was observed, although different patterns of staining were seen according to the degree of keratinization of the tumor cells. With the exception of the moderately differentiated OSCC group, Ki67 index showed significant correlation with the histologic pattern, increased proliferation being found in poorly differentiated OSCC ( P = 0.013). <\/jats:p>","DOI":"10.1354\/vp.42-1-66","type":"journal-article","created":{"date-parts":[[2005,1,18]],"date-time":"2005-01-18T20:33:44Z","timestamp":1106080424000},"page":"66-73","source":"Crossref","is-referenced-by-count":24,"title":["Immunohistochemical Studies of Epithelial Cell Proliferation and p53 Mutation in Bovine Ocular Squamous Cell Carcinoma"],"prefix":"10.1177","volume":"42","author":[{"given":"T.","family":"Carvalho","sequence":"first","affiliation":[{"name":"CIISA, Faculdade de Medicina Veterin\u00e0ria, Universidade T\u00e8cnica de Lisboa, Portugal"}]},{"given":"H.","family":"Vala","sequence":"additional","affiliation":[{"name":"Escola Superior Agr\u00e0ria de Viseu, Instituto Polit\u00e8cnico de Viseu, Portugal"}]},{"given":"C.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Direc\u00e7\u00e3o Regional de Desenvolvimento Agr\u00e0rio, Ponta Delgada, A\u00e7ores, Portugal"}]},{"given":"M.","family":"Pinho","sequence":"additional","affiliation":[{"name":"CIISA, Faculdade de Medicina Veterin\u00e0ria, Universidade T\u00e8cnica de Lisboa, Portugal"}]},{"given":"M. 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