{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T23:12:10Z","timestamp":1772925130228,"version":"3.50.1"},"reference-count":54,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2009,3,1]],"date-time":"2009-03-01T00:00:00Z","timestamp":1235865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:p> Fifty-six endothelial-derived urinary bladder tumor samples collected from 26 animals with bovine enzootic hematuria were selected for immunohistochemical studies. Expression of factor VHI-related antigen (FVIIIra), CD31, muscle-specific actin, uroplakin III (UPIII), and the cell cycle-related proteins cyclin Dl and p53 was evaluated in hemangiomas, \u201chemangioendotheliomas\u201d (a vascular tumor that histologically is intermediate in appearance between a hemangioma and a conventional hemangiosarcoma), and hemangiosarcomas. Although CD31 expression was seen in all endothelial tumors tested, FVIIIra was not expressed in poorly differentiated endothelial tumor cells from solid areas or in 7 muscle-invasive hemangiosarcomas. Cyclin Dl overexpression was seen in 53% of hemangiomas, 82% of hemangioendotheliomas, and 95% of hemangiosarcomas. P53 immunoreac-tivity was only seen in muscle-invasive hemangiosarcomas. The UPIII staining pattern, normally very intense on the apical aspect and cytoplasm of superficial urothelial cells, was altered in the urothelium in an estimated 25% of hemangiomas, most hemangioendotheliomas, and most hemangiosarcomas. In conclusion, CD31 is a better marker than FVIIIra in the characterization of bovine endothelial tumors. The cell cycle regulatory pathways involving cyclin Dl and p53 seem to be impaired in endothelial urinary bladder tumors, p53 immunoreactivity positively correlating with enhanced invasion. <\/jats:p>","DOI":"10.1354\/vp.46-2-211","type":"journal-article","created":{"date-parts":[[2009,3,4]],"date-time":"2009-03-04T09:03:55Z","timestamp":1236157435000},"page":"211-221","source":"Crossref","is-referenced-by-count":17,"title":["Immunohistochemical Evaluation of Vascular Urinary Bladder Tumors from                Cows with Enzootic Hematuria"],"prefix":"10.1177","volume":"46","author":[{"given":"T.","family":"Carvalho","sequence":"first","affiliation":[{"name":"CIISA, Faculdade de Medicina Veterin\u00e1ria, Universidade T\u00e9cnica de\r            Lisboa, Lisboa, Portugal"}]},{"given":"D.","family":"Naydan","sequence":"additional","affiliation":[{"name":"Veterinary Medical Teaching Hospital, School of Veterinary Medicine,\r            University of California, Davis"}]},{"given":"T.","family":"Nunes","sequence":"additional","affiliation":[{"name":"CIISA, Faculdade de Medicina Veterin\u00e1ria, Universidade T\u00e9cnica de\r            Lisboa, Lisboa, Portugal"}]},{"given":"C.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Servi\u00e7e de Desenvolvimento Agr\u00e1rio de S\u00e3o Miguel, Ponta Delgada, A\u00e7ores,\r            Portugal"}]},{"given":"M. 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