{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T00:56:13Z","timestamp":1793321773291,"version":"4.5.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study compares changes in bone microstructure in 6-month-old male GC-treated and female ovariectomized mice to their respective controls. In addition to a reduction in trabecular bone volume, GC treatment reduced bone mineral and elastic modulus of bone adjacent to osteocytes that was not observed in control mice nor estrogen-deficient mice. These microstructural changes in combination with the macrostructural changes could amplify the bone fragility in this metabolic bone disease.<\/jats:p>\n               <jats:p>Introduction: Patients with glucocorticoid (GC)-induced secondary osteoporosis tend to fracture at higher bone mineral densities than patients with postmenopausal osteoporosis. This suggests that GCs may alter bone material properties in addition to BMD and bone macrostructure.<\/jats:p>\n               <jats:p>Materials and Methods: Changes in trabecular bone structure, elastic modulus, and mineral to matrix ratio of the fifth lumbar vertebrae was assessed in prednisolone-treated mice and placebo-treated controls for comparison with estrogen-deficient mice and sham-operated controls. Compression testing of the third lumbar vertebrae was performed to assess whole bone strength.<\/jats:p>\n               <jats:p>Results: Significant reductions in trabecular bone volume and whole bone strength occurred in both prednisolone-treated and estrogen-deficient mice compared with controls after 21 days (p &amp;lt; 0.05). The average elastic modulus over the entire surface of each trabecula was similar in all the experimental groups. However, localized changes within the trabeculae in areas surrounding the osteocyte lacunae were observed only in the prednisolone-treated mice. The size of the osteocyte lacunae was increased, reduced elastic modulus around the lacunae was observed, and a \u201chalo\u201d of hypomineralized bone surrounding the lacunae was observed. This was associated with reduced (nearly 40%) mineral to matrix ratio determined by Raman microspectroscopy. These localized changes in elastic modulus and bone mineral to matrix ratio were not observed in the other three experimental groups.<\/jats:p>\n               <jats:p>Conclusions: Based on these results, it seems that GCs may have direct effects on osteocytes, resulting in a modification of their microenvironment. These changes, including an enlargement of their lacunar space and the generation of a surrounding sphere of hypomineralized bone, seem to produce highly localized changes in bone material properties that may influence fracture risk.<\/jats:p>","DOI":"10.1359\/jbmr.051103","type":"journal-article","created":{"date-parts":[[2006,4,26]],"date-time":"2006-04-26T13:26:15Z","timestamp":1146057975000},"page":"466-476","source":"Crossref","is-referenced-by-count":306,"title":["Glucocorticoid-Treated Mice Have Localized Changes in Trabecular Bone Material Properties and Osteocyte Lacunar Size That Are Not Observed in Placebo-Treated or Estrogen-Deficient Mice"],"prefix":"10.1093","volume":"21","author":[{"given":"Nancy E","family":"Lane","sequence":"first","affiliation":[{"name":"Department of Medicine, University of California at Davis, Sacramento, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California at Davis, Sacramento, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Balooch","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California at Davis, Sacramento, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi K","family":"Nalla","sequence":"additional","affiliation":[{"name":"Materials Science Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guive","family":"Balooch","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dental Sciences, University of California at San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Habelitz","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dental Sciences, University of California at San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John H","family":"Kinney","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dental Sciences, University of California at San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynda F","family":"Bonewald","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, University of Missouri at Kansas City School of Dentistry, Kansas City, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,12,4]]},"reference":[{"key":"2024013019393152000_bib1","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0889-857X(05)70196-4","article-title":"An update on glucocorticoid-induced osteoporosis","volume":"27","author":"Lane","year":"2001","journal-title":"Rheum Dis Clin North Am"},{"key":"2024013019393152000_bib2","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0889-8529(02)00064-6","article-title":"Glucocorticoid-induced osteoporosis","volume":"32","author":"Saag","year":"2003","journal-title":"Endocrinol Metab Clin North Am"},{"key":"2024013019393152000_bib3","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1136\/ard.54.1.49","article-title":"Rheumatoid arthritis, corticosteroid therapy, and hip fracture","volume":"54","author":"Cooper","year":"1995","journal-title":"Ann Rheum Dis"},{"key":"2024013019393152000_bib4","doi-asserted-by":"crossref","first-page":"3224","DOI":"10.1002\/art.11283","article-title":"Bone density threshold and other predictors of vertebral fractures in patients receiving oral glucocorticoid therapy","volume":"48","author":"Van Staa","year":"2003","journal-title":"Arthritis Rheum"},{"key":"2024013019393152000_bib5","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1007\/s001980200108","article-title":"The epidemiology of cortico-steroid osteoporosis. 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