{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:59:17Z","timestamp":1761512357156},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T00:00:00Z","timestamp":1177977600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Low vitamin D levels are common. Bone biopsies taken from 121 ambulant patients were therefore reviewed. Seasonal changes in mineralization correlated inversely with serum 25-hydroxyvitamin D but not the more active metabolite, 1,25-dihydroxyvitamin D. This implies that the latter is produced in bone.<\/jats:p>\n               <jats:p>Introduction: It has been 30 yr since a seasonal variation in osteoid surfaces and calcification fronts was noted in bone biopsies from hip fracture patients in Leeds and attributed to vitamin D status. It was suggested at that time that mild vitamin D deficiency might cause osteoporosis from malabsorption of calcium and more severe deficiency osteomalacia, but little has been published on this subject since.<\/jats:p>\n               <jats:p>Materials and Methods: We examined bone biopsies, calcium absorption data, and serum vitamin D metabolites in 121 patients attending our osteoporosis clinics in Adelaide. Biopsies were collected from the anterior iliac crest with a Jamshidi needle after two stat oral doses of 1 g of tetracycline 10 days apart, processed into plastic without demineralization, and all parameters were measured by point counting using a Weibel II graticule. Calcium absorption was measured after an oral dose of 5 \u03bcCi of 45Ca in 250 ml of water with 20 mg of calcium carrier. Serum 25-hydroxyvitamin D [25(OH)D] was measured by radioimmunoassay and 1,25-dihydroxyvitamin D [1,25(OH)2D] by radioimmunoassay after high-performance liquid chromatography (HPLC).<\/jats:p>\n               <jats:p>Results: 25(OH)D levels were lower from late autumn to early spring (April to September) than from late spring to early autumn (October to March) (51 \u00b1 23 versus 61 \u00b1 27 [SD] nM; p = 0.040). None of the biopsies yielded a diagnosis of osteomalacia, but osteoid thickness (O.Th.) was greater in the winter than the summer months (8.5 \u00b1 3.6 versus 7.1 \u00b1 2.8 \u03bcm; p = 0.015) as was mineralization lag time (MLT; 11.9 \u00b1 5.2 versus 9.5 \u00b1 3.6; p = 0.005). O.Th and log MLT were both inversely related to serum 25(OH)D (p = 0.014 and 0.036) but not serum 1,25(OH)2D. Calcium absorption was related to serum 1,25(OH)2D but not serum 25(OH)D.<\/jats:p>\n               <jats:p>Conclusions: We conclude that circulating 25(OH)D affects the mineralization process, whereas circulating 1,25(OH)2D affects bone indirectly through its effect on calcium absorption.<\/jats:p>","DOI":"10.1359\/jbmr.070203","type":"journal-article","created":{"date-parts":[[2007,2,5]],"date-time":"2007-02-05T20:09:06Z","timestamp":1170706146000},"page":"757-761","source":"Crossref","is-referenced-by-count":36,"title":["Seasonal Change in Osteoid Thickness and Mineralization Lag Time in Ambulant Patients"],"prefix":"10.1093","volume":"22","author":[{"given":"Allan G","family":"Need","sequence":"first","affiliation":[{"name":"Hanson Institute, Adelaide, South Australia"},{"name":"Discipline of Medicine, University of Adelaide, Adelaide, South Australia"},{"name":"Institute of Medical and Veterinary Science, Adelaide, South 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