{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:02:10Z","timestamp":1762671730530},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2009,4,1]],"date-time":"2009-04-01T00:00:00Z","timestamp":1238544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Hyperhomocysteinemia (HHCY) has been linked to fragility fractures and osteoporosis. Folate and vitamin B12 deficiencies are among the main causes of HHCY. However, the impact of these vitamins on bone health has been poorly studied. This study analyzed the effect of folate and vitamin B12 deficiency on bone in rats. We used two groups of rats: a control group (Co, n = 10) and a vitamin-deficient group (VitDef, n = 10). VitDef animals were fed for 12 wk with a folate- and vitamin B12\u2013free diet. Co animals received an equicaloric control diet. Tissue and plasma concentrations of homocysteine (HCY), S-adenosyl-homocysteine (SAH), and S-adenosyl-methionine (SAM) were measured. Bone quality was assessed by biomechanical testing (maximum force of an axial compression test; Fmax), histomorphometry (bone area\/total area; B.Ar.\/T.Ar.], and the measurement of biochemical bone turnover markers (osteocalcin, collagen I C-terminal cross-laps [CTX]). VitDef animals developed significant HHCY (Co versus VitDef: 6.8 \u00b1 2.7 versus 61.1 \u00b1 12.8 \u03bcM, p &amp;lt; 0.001) that was accompanied by a high plasma concentration of SAH (Co versus VitDef: 24.1 \u00b1 5.9 versus 86.4 \u00b1 44.3 nM, p &amp;lt; 0.001). However, bone tissue concentrations of HCY, SAH, and SAM were similar in the two groups. Fmax, B.Ar.\/T.Ar., OC, and CTX did not differ between VitDef and Co animals, indicating that bone quality was not affected. Folate and vitamin B12 deficiency induces distinct HHCY but has no effect on bone health in otherwise healthy adult rats. The unchanged HCY metabolism in bone is the most probable explanation for the missing effect of the vitamin-free diet on bone.<\/jats:p>","DOI":"10.1359\/jbmr.081211","type":"journal-article","created":{"date-parts":[[2008,12,2]],"date-time":"2008-12-02T17:32:07Z","timestamp":1228239127000},"page":"589-596","source":"Crossref","is-referenced-by-count":21,"title":["Experimental Folate and Vitamin B12 Deficiency Does Not Alter Bone Quality in Rats"],"prefix":"10.1093","volume":"24","author":[{"given":"Markus","family":"Herrmann","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine, University Hospital of Saarland, Homburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Britt","family":"Wildemann","sequence":"additional","affiliation":[{"name":"Julius Wolff Institut, Charit\u00e9-Universit\u00e4tsmedizin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine, University Hospital of Saarland, Homburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Wolny","sequence":"additional","affiliation":[{"name":"Julius Wolff Institut, Charit\u00e9-Universit\u00e4tsmedizin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heike","family":"Schorr","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine, University Hospital of Saarland, Homburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omid","family":"Taban-Shomal","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine, University Hospital of Saarland, Homburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Umanskaya","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine, University Hospital of Saarland, Homburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steffen","family":"Ross","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine, University Hospital of Saarland, Homburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patric","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Trauma, Hand, and Reconstructive Surgery, University Hospital of Saarland, Homburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"H\u00fcbner","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine, University Hospital of Saarland, Homburg, 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