{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T21:11:47Z","timestamp":1774645907403,"version":"3.50.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>RANKL is essential for the terminal differentiation of monocytes\/marcrophages into osteoclasts. RANKL induces long-lasting oscillations in the intracellular concentration of Ca2+ ([Ca2+]i) only after 24 h of stimulation. These Ca2+ oscillations play a switch-on role in NFATc1 expression and osteoclast differentiation. Which Ca2+ transporting pathway is induced by RANKL to evoke the Ca2+ oscillations and its specific role in RANKL-mediated osteoclast differentiation is not known. This study examined the effect of a partial loss of sarco\/endoplasmic reticulum Ca2+ ATPase type2 (SERCA2) on osteoclast differentiation in SERCA2 heterozygote mice (SERCA2+\/\u2212). The BMD in the tibias of SERCA2+\/\u2212 mice increased &amp;gt;1.5-fold compared with wildtype mice (WT). RANKL-induced [Ca2+]i oscillations were generated 48 h after RANKL treatment in the WT mice but not in the SERCA2+\/\u2212 bone marrow\u2013derived macrophages (BMMs). Forty-eight hours after RANKL treatment, there was a lower level of NFATc1 protein expression and markedly reduced translocation of NFATc1 into the nucleus during osteoclastogenesis of the SERCA2+\/\u2212 BMMs. In addition, RANKL treatment of SERCA2+\/\u2212 BMMs incompletely induced formation of multinucleated cells, leading to reduced bone resorption activity. These results suggest that RANKL-mediated induction of SERCA2 plays a critical role in the RANKL-induced [Ca2+]i oscillations that are essential for osteoclastogenesis.<\/jats:p>","DOI":"10.1359\/jbmr.090420","type":"journal-article","created":{"date-parts":[[2009,5,6]],"date-time":"2009-05-06T18:15:04Z","timestamp":1241633704000},"page":"1763-1769","source":"Crossref","is-referenced-by-count":32,"title":["Alteration of RANKL-Induced Osteoclastogenesis in Primary Cultured Osteoclasts From SERCA2+\/\u2212 Mice"],"prefix":"10.1093","volume":"24","author":[{"given":"Yu-Mi","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Oral Biology, Brain Korea 21 Project, Center for Natural Defense System, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Seuk","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Brain Korea 21 Project, Center for Natural Defense System, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aran","family":"Son","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Brain Korea 21 Project, Center for Natural Defense System, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong Hee","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Brain Korea 21 Project, Center for Natural Defense System, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung-Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Yonsei University College of Dentistry, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong Taeg","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Brain Korea 21 Project, Center for Natural Defense System, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syng-Ill","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Brain Korea 21 Project, Center for Natural Defense System, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Min","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Brain Korea 21 Project, Center for Natural Defense System, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2009,12,4]]},"reference":[{"key":"2024020319555200200_bib1","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1196\/annals.1402.071","article-title":"The role of NFAT in osteoclast formation","volume":"1116","author":"Takayanagi","year":"2007","journal-title":"Ann NY Acad 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