{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T16:54:17Z","timestamp":1765040057834},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[1998,7,1]],"date-time":"1998-07-01T00:00:00Z","timestamp":899251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Interleukin-1 (IL-1) inhibits collagen synthesis in osteoblastic cell lines and primary osteoblast-like cells. However, promoter elements regulating type I collagen A1 (COLIA1) expression in vivo and in organ culture may differ from those regulating expression in cell culture. We have examined the effects of IL-1 on reporter gene activity in neonatal transgenic mouse calvariae bearing COLIA1 promoter-chloramphenicol acetyltransferase (ColCAT) fusion genes. The parent construct, ColCAT 3.6, contains 3.5 kb of 5\u2032 flanking sequence and 115 bp of 5\u2032 untranslated region fused to the CAT reporter. In 48-h calvarial organ cultures, IL-1 repressed ColCAT 3.6 promoter activity and collagen synthesis in a dose-related manner, with a maximal inhibition of 40\u201365%. This repression was retained in 5\u2032 deletion constructs truncated to \u22121719 bp. The inhibition of transgene mRNA was blocked by cycloheximide, indicating a requirement for new protein synthesis. Pretreatment with indomethacin diminished the inhibitory effect of IL-1 on CAT activity and collagen synthesis, suggesting partial mediation by prostaglandins. Local in vivo injection of IL-1 (500 ng) decreased calvarial transgene mRNA after 8 h, an effect that was partially blocked by indomethacin. ColCAT transgenic mice represent a useful model for in vitro and in vivo assessment of COLIA1 promoter regulation by cytokines and other factors.<\/jats:p>","DOI":"10.1359\/jbmr.1998.13.7.1076","type":"journal-article","created":{"date-parts":[[2006,4,27]],"date-time":"2006-04-27T00:01:11Z","timestamp":1146096071000},"page":"1076-1083","source":"Crossref","is-referenced-by-count":14,"title":["Interleukin-1 Represses COLIA1 Promoter Activity in Calvarial Bones of Transgenic ColCAT Mice In Vitro and In Vivo"],"prefix":"10.1093","volume":"13","author":[{"given":"John R.","family":"Harrison","sequence":"first","affiliation":[{"name":"Department of Medicine, The University of Connecticut Health Center, Farmington, Connecticut, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa M.","family":"Kleinert","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Connecticut Health Center, Farmington, Connecticut, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Penny L.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Connecticut Health Center, Farmington, Connecticut, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul H.","family":"Krebsbach","sequence":"additional","affiliation":[{"name":"Present address: Department of Oral Medicine, Pathology and Surgery, School of Dentistry, University of Michigan, Ann Arbor, Michigan, U.S.A."},{"name":"Department of Medicine, The University of Connecticut Health Center, Farmington, Connecticut, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Woody","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Connecticut, Storrs, Connecticut, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Clark","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center, Newington, Connecticut, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David W.","family":"Rowe","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The University of Connecticut Health Center, Farmington, Connecticut, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander C.","family":"Lichtler","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The University of Connecticut Health Center, Farmington, Connecticut, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara E.","family":"Kream","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Connecticut Health Center, Farmington, Connecticut, 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