{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:17:05Z","timestamp":1790097425596,"version":"4.0.1"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[1998,9,1]],"date-time":"1998-09-01T00:00:00Z","timestamp":904608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The calcium-sensing receptor (CaR) is a G protein-coupled receptor that plays key roles in extracellular calcium ion (Ca2+o) homeostasis in parathyroid gland and kidney. Macrophage-like mononuclear cells appear at sites of osteoclastic bone resorption during bone remodeling and may play a role in the \u201creversal\u201d phase following osteoclastic resorption and preceding bone formation. Bone resorption produces substantial local increases in Ca2+o that could provide a signal for bone marrow mononuclear cells in the vicinity, leading us to investigate whether such mononuclear cells express the CaR. In this study, we used the mouse J774 cell line, which exhibits a pure monocyte-macrophage phenotype. Both immunocytochemistry and Western blot analysis, using polyclonal antisera specific for the CaR, detected CaR protein in J774 cells. The use of reverse transcriptase-polymerase chain reaction with CaR-specific primers, including a set of intron-spanning primers, followed by nucleotide sequencing of the amplified products, also identified CaR transcripts in J774 cells. Exposure of J774 cells to high Ca2+o (2.8 mM or more) or the polycationic CaR agonist, neomycin (100 \u03bcM), stimulated both chemotaxis and DNA synthesis in J774 cells. Therefore, taken together, our data strongly suggest that the monocyte-macrophage cell line, J774, possesses both CaR protein and mRNA very similar, if not identical, to those in parathyroid and kidney.<\/jats:p>","DOI":"10.1359\/jbmr.1998.13.9.1390","type":"journal-article","created":{"date-parts":[[2006,4,27]],"date-time":"2006-04-27T00:05:00Z","timestamp":1146096300000},"page":"1390-1397","source":"Crossref","is-referenced-by-count":47,"title":["Extracellular Calcium (Ca2+o)-Sensing Receptor in a Mouse Monocyte-Macrophage Cell Line (J774): Potential Mediator of the Actions of Ca2+o on the Function of J774 Cells"],"prefix":"10.1093","volume":"13","author":[{"given":"Toru","family":"Yamaguchi","sequence":"first","affiliation":[{"name":"Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Kifor","sequence":"additional","affiliation":[{"name":"Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naibedya","family":"Chattopadhyay","sequence":"additional","affiliation":[{"name":"Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Bai","sequence":"additional","affiliation":[{"name":"Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward M.","family":"Brown","sequence":"additional","affiliation":[{"name":"Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,12,4]]},"reference":[{"key":"2024020713274320600_bib1","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1038\/366575a0","article-title":"Cloning and characterization of an extracellular Ca2+-sensing receptor from bovine parathyroid","volume":"366","author":"Brown","year":"1993","journal-title":"Nature"},{"key":"2024020713274320600_bib2","doi-asserted-by":"crossref","first-page":"12919","DOI":"10.1074\/jbc.270.21.12919","article-title":"Molecular cloning and characterization of the human parathyroid calcium receptor","volume":"270","author":"Garrett","year":"1995","journal-title":"J Biol Chem"},{"key":"2024020713274320600_bib3","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1073\/pnas.92.1.131","article-title":"Cloning and functional expression of a rat kidney extracellular calcium\/polyvalent cation-sensing receptor","volume":"92","author":"Riccardi","year":"1995","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024020713274320600_bib4","doi-asserted-by":"crossref","first-page":"5202","DOI":"10.1210\/endo.136.11.7588259","article-title":"Calcitonin-secreting cells of the thyroid gland express an extracellular calcium-sensing receptor gene","volume":"136","author":"Garrett","year":"1995","journal-title":"Endocrinology"},{"key":"2024020713274320600_bib5","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1016\/0092-8674(93)90617-Y","article-title":"Mutations in the human Ca2+-sensing receptor gene cause familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism [see comments]","volume":"75","author":"Pollak","year":"1993","journal-title":"Cell"},{"key":"2024020713274320600_bib6","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1038\/ng1194-303","article-title":"Autosomal dominant hypocalcaemia caused by a Ca2+-sensing receptor gene mutation","volume":"8","author":"Pollak","year":"1994","journal-title":"Nat Genet"},{"key":"2024020713274320600_bib7","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/0014-4827(88)90191-7","article-title":"Microelectrode studies on the acid microenvironment beneath adherent macrophages and osteoclasts","volume":"175","author":"Silver","year":"1988","journal-title":"Exp Cell Res"},{"key":"2024020713274320600_bib8","first-page":"175","volume-title":"Bone and Mineral Research. 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