{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T19:09:05Z","timestamp":1769281745277,"version":"3.49.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2000,12,1]],"date-time":"2000-12-01T00:00:00Z","timestamp":975628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Bisphosphonates inhibit bone resorption by reducing osteoclastic cell number and activity. Alendronate is a nitrogen-containing bisphosphonate analog used in the treatment of postmenopausal osteoporosis. The effects of alendronate in osteoclasts are well documented; however, there is limited information on the actions of alendronate in osteoblasts (Ob's). In this study, we investigated the effects of alendronate at concentrations of 1-100 \u03bcM on the synthesis of collagenase 3 or matrix metalloproteinase 13 (MMP-13) and tissue inhibitors of MMPs (TIMPs) 1, 2, and 3 in primary Ob-enriched cells from 22-day-old fetal rat calvariae. Alendronate at concentrations higher than 10 \u03bcM markedly stimulated the synthesis of collagenase messenger RNA (mRNA) and immunoreactive protein in Ob's. Alendronate did not stimulate the transcriptional rate of the collagenase 3 gene. However, in transcriptionally arrested cells, alendronate prolonged the half-life of collagenase transcripts. Alendronate did not alter the expression of TIMP 1 and 2, but modestly stimulated the expression of TIMP 3. The actions of alendronate in Ob's suggest potential additional effects in bone remodeling.<\/jats:p>","DOI":"10.1359\/jbmr.2000.15.12.2345","type":"journal-article","created":{"date-parts":[[2006,4,27]],"date-time":"2006-04-27T02:33:34Z","timestamp":1146105214000},"page":"2345-2351","source":"Crossref","is-referenced-by-count":18,"title":["Alendronate Stimulates Collagenase 3 Expression in Osteoblasts by Posttranscriptional Mechanisms"],"prefix":"10.1093","volume":"15","author":[{"given":"Samuel","family":"Varghese","sequence":"first","affiliation":[{"name":"University of Connecticut School of Medicine, Farmington, Connecticut, U.S.A."},{"name":"Departments of Research and Medicine, Saint Francis Hospital and Medical Center, Hartford, Connecticut, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernesto","family":"Canalis","sequence":"additional","affiliation":[{"name":"University of Connecticut School of Medicine, Farmington, Connecticut, 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