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This action involves the well\u2010characterized nuclear 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> receptor. However, it has been recognized that several cellular responses to 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> may not to be related to the exclusive nuclear receptor. Indeed, this secosteroid is able to generate rapid responses that have been proposed to be mediated by interactions of the ligand, which is a putative cell membrane\u2010associated rapid\u2010response steroid (MARRS) binding protein for 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> [1,25D<jats:sub>3<\/jats:sub>\u2010MARRS]. The nongenomic pathway of 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> was studied here in detail by immunolocalization of the 1,25D<jats:sub>3<\/jats:sub>\u2010MARRS during the specific context of human prenatal development. Western blotting with proteins extracted from 4 week\u2010 to 27\u2010week\u2010old embryos was performed, evidencing a 65\u2010kDa molecular species recognized by antibody Ab 099 generated against synthetic peptides corresponding to the N terminus of the 1,25D<jats:sub>3<\/jats:sub>\u2010MARRS from chick intestinal basolateral membranes. Based on this biochemical conservation of protein in the human species, the temporospatial expression patterns were established in the craniofacial skeleton at the same ages. Comparative analysis was performed in teeth and bones from early morphogenesis to terminal cell differentiation and extracellular biomineralization. The data show the potential implication of 1,25D<jats:sub>3<\/jats:sub>\u2010MARRS in the heterogeneous cell population including ameloblasts, odontoblasts, osteoblasts, and osteoclasts. The epithelial\u2010mesenchymal cascade related to odontogenesis was coincident with a sequence of up\u2010 and down\u2010regulation of immunoreactive 1,25D<jats:sub>3<\/jats:sub>\u2010MARRS. Biomineralization was associated with a striking up\u2010regulation in the adjoining secretory cells in all tissues. Finally, osteoclasts appeared also to express the 1,25D<jats:sub>3<\/jats:sub>\u2010MARRS during these early phases of bone modeling. Previously obtained data of the nuclear vitamin D receptor (VDR) expression and this study on 1,25D<jats:sub>3<\/jats:sub>\u2010MARRS suggest the existence of cross\u2010talk between the genomic and nongenomic pathways during human development.<\/jats:p>","DOI":"10.1359\/jbmr.2002.17.9.1588","type":"journal-article","created":{"date-parts":[[2006,4,27]],"date-time":"2006-04-27T05:28:41Z","timestamp":1146115721000},"page":"1588-1596","source":"Crossref","is-referenced-by-count":37,"title":["Expression of a 1,25\u2010Dihydroxyvitamin D<sub>3<\/sub> Membrane\u2010Associated Rapid\u2010Response Steroid Binding Protein During Human Tooth and Bone Development and Biomineralization"],"prefix":"10.1093","volume":"17","author":[{"given":"Mohand","family":"Mesbah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilka","family":"Nemere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petros","family":"Papagerakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Raphael","family":"Nefussi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Orestes\u2010Cardoso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Nessmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Berdal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,12,2]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-128X(98)00091-9"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00008480-199808000-00019"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869931"},{"key":"e_1_2_5_5_2","doi-asserted-by":"crossref","first-page":"23750","DOI":"10.1016\/S0021-9258(17)31579-X","article-title":"Identification 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