{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:49:19Z","timestamp":1791305359092,"version":"4.3.1"},"reference-count":108,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Bone &amp; Mineral Res"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The presence of estrogen receptor \u03b1 (ER\u03b1) in osteocytes was identified immunocytochemically in transverse sections from 560 to 860 \u03bcm distal to the midshaft of normal neonatal and adult male and female rat ulnas (<jats:italic>n<\/jats:italic> = 3 of each) and from adult male rat ulnas that had been exposed to 10 days of in vivo daily 10\u2010minute periods of cyclic loading producing peak strains of either \u22123000 (<jats:italic>n<\/jats:italic> = 3) or \u22124000 microstrain (<jats:italic>n<\/jats:italic> = 5). Each animal ambulated normally between loading periods, and its contralateral ulna was used as a control. In animals in which limbs were subject to normal locomotor loading alone, 14 \u00b11.2% SEM of all osteocytes in each bone section were ER\u03b1 positive. There was no influence of either gender (<jats:italic>p<\/jats:italic> = 0.725) or age (<jats:italic>p<\/jats:italic> = 0.577) and no interaction between them (<jats:italic>p<\/jats:italic> = 0.658). In bones in which normal locomotion was supplemented by short periods of artificial loading, fewer osteocytes expressed ER\u03b1 (7.5 \u00b1 0.91% SEM) than in contralateral control limbs, which received locomotor loading alone (14 \u00b1 1.68% SEM; <jats:italic>p<\/jats:italic> = 0.01; median difference, 6.43; 95% CI, 2.60, 10.25). The distribution of osteocytes expressing ER\u03b1 was uniform across all sections and thus did not reflect local peak strain magnitude. This suggests that osteocytes respond to strain as a population, rather than as individual strain\u2010responsive cells. These data are consistent with the hypothesis that ER\u03b1 is involved in bone cells' responses to mechanical strain. High strains appear to decrease ER\u03b1 expression. In osteoporotic bone, the high strains assumed to accompany postmenopausal bone loss may reduce ER\u03b1 levels and therefore impair the capacity for appropriate adaptive remodeling.<\/jats:p>","DOI":"10.1359\/jbmr.2002.17.9.1646","type":"journal-article","created":{"date-parts":[[2006,4,27]],"date-time":"2006-04-27T05:28:41Z","timestamp":1146115721000},"page":"1646-1655","source":"Crossref","is-referenced-by-count":56,"title":["The Effect of In Vivo Mechanical Loading on Estrogen Receptor \u03b1 Expression in Rat Ulnar Osteocytes"],"prefix":"10.1093","volume":"17","author":[{"given":"P. J.","family":"Ehrlich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. S.","family":"Noble","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. L.","family":"Jessop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. Y.","family":"Stevens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. R.","family":"Mosley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. E.","family":"Lanyon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,12,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9290(99)00173-6"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650101016"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007740050070"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/096120339900800512"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1998.274.6.E1113"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650110802"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1007\/BF02210682","article-title":"Osteoporosis prevention and treatment with sex hormone replacement therapy","volume":"14","author":"Rozenberg S","year":"1995","journal-title":"Clin Rheumatol"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10942"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.83.7.2266"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199410203311604"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.8.1526"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.9.1835"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199809103391104"},{"key":"e_1_2_6_15_2","first-page":"275","article-title":"Skeletal effects of estrogen","volume":"15","author":"Turner RT","year":"1994","journal-title":"Endocr Rev"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-125-2-810"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(00)00414-2"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01674805"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-199701000-00002"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(96)90162-3"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(96)00227-X"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650100914"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8480174"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.11.2169"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.4.489"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1096-1132"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(99)00420-9"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.82.9.3128"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1998.13.8.1243"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9290(82)90246-9"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9290(84)90003-4"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02406134"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-198466030-00012"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02553711"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02406149"},{"key":"e_1_2_6_36_2","first-page":"147","article-title":"Disuse osteoporosis","volume":"48","author":"Takata S","year":"2001","journal-title":"J Med Invest"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1999.87.1.189"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00298562"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.8.1437"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.8.1429"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(98)00189-6"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(94)90291-7"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001980070021"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001980170134"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-6009(08)80139-0"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00316288"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.1.166"},{"key":"e_1_2_6_48_2","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1007\/s004210050602","article-title":"Does endurance running before orchidectomy prevent osteopenia in rats?","volume":"80","author":"Horcajada\u2010Molteni MN","year":"1999","journal-title":"Eur J Appl Physiol Occup Physiol"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(00)00377-X"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650071403"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01673415"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092190104"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007760050054"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650090606"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.8.4199"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650110411"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9290(95)00089-5"},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(95)00378-9"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1998.13.8.1275"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.10.1742"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(00)00324-0"},{"key":"e_1_2_6_62_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.6.1045"},{"key":"e_1_2_6_63_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.11.1937"},{"key":"e_1_2_6_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(96)00385-7"},{"key":"e_1_2_6_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(01)00626-3"},{"key":"e_1_2_6_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(98)00113-6"},{"key":"e_1_2_6_67_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00301686"},{"issue":"2","key":"e_1_2_6_68_2","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1042\/bj3150407","article-title":"Protein tyrosine kinases regulate agonist\u2010stimulated prostacyclin release but not von Willebrand factor secretion from human umbilical vein endothelial cells","volume":"315","author":"Wheeler\u2010Jones CP","year":"1996","journal-title":"Biochem J"},{"key":"e_1_2_6_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(00)00246-5"},{"key":"e_1_2_6_70_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3164526"},{"key":"e_1_2_6_71_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3388021"},{"key":"e_1_2_6_72_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650100112"},{"key":"e_1_2_6_73_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(96)00346-8"},{"key":"e_1_2_6_74_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199907)188:3<294::AID-PATH361>3.0.CO;2-Y"},{"key":"e_1_2_6_75_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.83.7.2421"},{"key":"e_1_2_6_76_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s004180050342","article-title":"Expression of estrogen receptor\u2010alpha in cells of the osteoclastic lineage","volume":"111","author":"Oreffo ROC","year":"1999","journal-title":"Histochemistry"},{"key":"e_1_2_6_77_2","unstructured":"GeversEF van der EerdenBC L\u00f6wikCWGM WitJ1999Estrogen receptor and cytochrome p450 aromatase is present in the male and female growth plate of the rat. Ph.D. Thesis University of Leiden The Netherlands pp.190\u2013206."},{"key":"e_1_2_6_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(98)00148-3"},{"key":"e_1_2_6_79_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01351835"},{"key":"e_1_2_6_80_2","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH684>3.0.CO;2-P"},{"key":"e_1_2_6_81_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.7.1189"},{"key":"e_1_2_6_82_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(00)00246-5"},{"key":"e_1_2_6_83_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M909675199"},{"key":"e_1_2_6_84_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113125"},{"key":"e_1_2_6_85_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.11.2222"},{"key":"e_1_2_6_86_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.1.128"},{"key":"e_1_2_6_87_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.11.2231"},{"key":"e_1_2_6_88_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03347333"},{"key":"e_1_2_6_89_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.12.2479"},{"key":"e_1_2_6_90_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2265.2000.00979.x"},{"key":"e_1_2_6_91_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.84.12.4597"},{"key":"e_1_2_6_92_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0864"},{"key":"e_1_2_6_93_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.9.4037"},{"key":"e_1_2_6_94_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.5.1858"},{"key":"e_1_2_6_95_2","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(94)90072-8"},{"key":"e_1_2_6_96_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(97)00047-2"},{"key":"e_1_2_6_97_2","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1420307"},{"key":"e_1_2_6_98_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.4.5897"},{"key":"e_1_2_6_99_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.138.11.5652"},{"key":"e_1_2_6_100_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02106093"},{"key":"e_1_2_6_101_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650100906"},{"key":"e_1_2_6_102_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650080605"},{"key":"e_1_2_6_103_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1998.84.5.1506"},{"issue":"1","key":"e_1_2_6_104_2","first-page":"37","article-title":"Effects of Estrogens and SERMS on Bone","volume":"19","author":"Vanin C","year":"1997","journal-title":"J SOGC"},{"key":"e_1_2_6_105_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002239900536"},{"key":"e_1_2_6_106_2","first-page":"167","article-title":"The microscopic determinants of bone mechanical properties","volume":"8","author":"Marotti G","year":"1994","journal-title":"Ital J Miner Electrolyte Metab"},{"key":"e_1_2_6_107_2","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(92)90452-3"},{"key":"e_1_2_6_108_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(99)00241-0"},{"key":"e_1_2_6_109_2","first-page":"133","article-title":"The osteocyte as a wiring transmission system","volume":"1","author":"Marotti G","year":"2001","journal-title":"J Musculoskel Neuron Interact"}],"container-title":["Journal of Bone and Mineral Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1359%2Fjbmr.2002.17.9.1646","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full\/10.1359\/jbmr.2002.17.9.1646","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T15:05:37Z","timestamp":1710342337000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmr\/article\/17\/9\/1646-1655\/7610741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9]]},"references-count":108,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2002,9]]}},"alternative-id":["10.1359\/jbmr.2002.17.9.1646"],"URL":"https:\/\/doi.org\/10.1359\/jbmr.2002.17.9.1646","archive":["Portico"],"relation":{},"ISSN":["0884-0431","1523-4681"],"issn-type":[{"value":"0884-0431","type":"print"},{"value":"1523-4681","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,9]]}}}