{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T18:59:05Z","timestamp":1782154745418,"version":"3.54.5"},"reference-count":25,"publisher":"Optica Publishing Group","issue":"31","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1364\/OA_License_v2#VOR"},{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/opg.optica.org\/policies\/opg-tdm-policy.json"}],"funder":[{"name":"China Manned Space Program","award":["HYZHXMN01006"],"award-info":[{"award-number":["HYZHXMN01006"]}]}],"content-domain":{"domain":["opg.optica.org"],"crossmark-restriction":false},"short-container-title":["Appl. Opt."],"published-print":{"date-parts":[[2025,11,1]]},"abstract":"<jats:p>\n                    The random positioning machine (RPM) is used to simulate microgravity for biodynamical study via stochastic movements, during which real-time imaging\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    is highly desired. However, the jitter induced by RPM rotating complicates stable imaging of live samples. In this paper, we develop an extended depth-of-field (DOF) fluorescence microscope (EDOFM) for stable imaging during the operation of RPM. By interposing a 5\u00a0mm thick glass plate between the objective lens and the sample, we purposely induce spherical aberration to extend the DOF. We also integrate a low-numerical-aperture (NA) light stop to mitigate the reduction of imaging clarity caused by spherical aberration. Our design extends the DOF of the EDOFM by over 15-fold compared to conventional wide-field setups, verified by both ZEMAX simulations and practical experiments. We assessed our EDOFM system with transgenic zebrafish larva\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    for blood cell tracking, under both stationary and rotating conditions of the RPM. With the EDOFM, we captured more signals during RPM operation, demonstrating its superior imaging stability during RPM-induced jitter. We expect broad applications of the EDOFM system in the study of microgravity.\n                  <\/jats:p>","DOI":"10.1364\/ao.571445","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T15:00:29Z","timestamp":1759849229000},"page":"9215","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Extended depth-of-field fluorescence microscope for stable imaging during random positioning machine running"],"prefix":"10.1364","volume":"64","author":[{"given":"Andy","family":"Yu Shi","sequence":"first","affiliation":[{"name":"Tsinghua University High School"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Tang","sequence":"additional","affiliation":[{"name":"Tsinghua University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8250-7547","authenticated-orcid":true,"given":"Lingjie","family":"Kong","sequence":"additional","affiliation":[{"name":"Tsinghua University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2025,10,21]]},"reference":[{"key":"ao-64-31-9215-R1","doi-asserted-by":"publisher","first-page":"971474","DOI":"10.1155\/2015\/971474","type":"journal-article","volume":"2015","author":"Wuest","year":"2015","journal-title":"Biomed. 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Biophotonics"},{"key":"ao-64-31-9215-R10","doi-asserted-by":"publisher","first-page":"90","DOI":"10.3390\/aerospace12020090","type":"journal-article","volume":"12","author":"Shi","year":"2025","journal-title":"Aerospace"},{"key":"ao-64-31-9215-R11","doi-asserted-by":"publisher","first-page":"3805","DOI":"10.1364\/OL.490087","type":"journal-article","volume":"48","author":"Tucker","year":"2023","journal-title":"Opt. Lett."},{"key":"ao-64-31-9215-R12","doi-asserted-by":"publisher","first-page":"2035","DOI":"10.1364\/BOE.445775","type":"journal-article","volume":"13","author":"Flores-Valle","year":"2022","journal-title":"Biomed. Opt. Express"},{"key":"ao-64-31-9215-R13","doi-asserted-by":"publisher","first-page":"3717","DOI":"10.1364\/BOE.425588","type":"journal-article","volume":"12","author":"Valera","year":"2021","journal-title":"Biomed. Opt. 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All rights, including for text and data mining (TDM), Artificial Intelligence (AI) training, and similar technologies, are reserved.","name":"copyright","label":"Copyright"}]}}