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Opt."],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:p>\n                    Fluorescence scanning laser ophthalmoscopy (SLO) provides\n\t\t\t\t\thigh-resolution\n                    <jats:italic toggle=\"yes\">en face<\/jats:italic>\n                    imaging of the\n\t\t\t\t\tretina to investigate retinal ganglion cells (RGCs) in both small\n\t\t\t\t\tanimals and humans. Multicolor fluorescence SLO can further extend the\n\t\t\t\t\tanatomical and functional imaging capabilities; however, its\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    implementation remains challenging due\n\t\t\t\t\tto optical aberrations, limited labeling strategies, and the need for\n\t\t\t\t\tmultichannel calibration. In this study, we present a compact\n\t\t\t\t\ttwo-color fluorescence SLO system designed with registered\n\t\t\t\t\tfield-of-views (FOVs) between the two color channels for\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    imaging of RGCs at single-cell\n\t\t\t\t\tresolution in mice. Using an Eno2-YFP transgenic mouse model with\n\t\t\t\t\tdextran labeling of the RGCs, we demonstrate high-quality two-color\n\t\t\t\t\timaging across a 1.2\u00d71.2mm\n                    <jats:sup>2<\/jats:sup>\n                    FOV. Furthermore, by employing\n\t\t\t\t\tadeno-associated virus (AAV)-mediated co-labeling of RGCs and\n\t\t\t\t\tmitochondria, we achieved high-resolution visualization and image\n\t\t\t\t\toverlay with precise two-channel registration. The two-color\n\t\t\t\t\tfluorescence SLO system holds promise for longitudinal studies of\n\t\t\t\t\tRGCs, with applications in neurodegeneration, glaucoma, and\n\t\t\t\t\ttherapeutic development.\n                  <\/jats:p>","DOI":"10.1364\/ao.578128","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T17:00:07Z","timestamp":1763139607000},"page":"252","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Two-color fluorescence scanning laser ophthalmoscopy for\n                    <i>in vivo<\/i>\n                    mouse retinal ganglion cell imaging at the single-cell level"],"prefix":"10.1364","volume":"65","author":[{"given":"Tianyu","family":"Xu","sequence":"first","affiliation":[{"name":"Northwestern University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijia","family":"Fan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/000e0be47","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Kuranov","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/000e0be47","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Opticent Inc."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Sun","sequence":"additional","affiliation":[{"name":"Northwestern University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5089-1196","authenticated-orcid":true,"given":"Hao F.","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/000e0be47","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"ao-65-1-252-R1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.imlet.2014.04.003","type":"journal-article","volume":"161","author":"Eissing","year":"2014","journal-title":"Immunol.\n\t\t\t\t\tLett."},{"key":"ao-65-1-252-R2","doi-asserted-by":"publisher","first-page":"e68","DOI":"10.1002\/cpcy.68","type":"journal-article","volume":"92","author":"Elliott","year":"2020","journal-title":"Curr.\n\t\t\t\t\tProtoc. 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