{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T02:00:04Z","timestamp":1768874404521,"version":"3.49.0"},"reference-count":42,"publisher":"Optica Publishing Group","issue":"10","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1364\/OA_License_v1#VOR-OA"},{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/opg.optica.org\/policies\/opg-tdm-policy.json"}],"funder":[{"DOI":"10.13039\/501100010982","name":"Philippine Council for Industry, Energy, and Emerging Technology Research and Development","doi-asserted-by":"publisher","award":["04125"],"award-info":[{"award-number":["04125"]}],"id":[{"id":"10.13039\/501100010982","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["opg.optica.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Opt. Express"],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:p>We studied the elastic profile of monocytic THP-1 leukemia cells using a microfluidic-assisted optical trap. A 2-<jats:italic>\u00b5<\/jats:italic>m fused silica bead was optically trapped to mechanically dent an immobilized single THP-1 monocyte sieved on a 15-\u00b5m microfluidic capture chamber. Cells treated with Zeocin and untreated cells underwent RT-qPCR analysis to determine cell apoptosis through gene expression in relation to each cell\u2019s deformation profile. Results showed that untreated cells with 43.05 \u00b1 6.68\u2005Pa are more elastic compared to the treated cells with 15.81 \u00b1 2.94\u2005Pa. THP-1 monocyte\u2019s elastic modulus is indicative of cell apoptosis shown by upregulated genes after Zeocin treatment. This study clearly showed that the developed technique can be used to distinguish between cells undergoing apoptosis and cells not undergoing apoptosis and which may apply to the study of other cells and other cell states as well.<\/jats:p>","DOI":"10.1364\/boe.402526","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T07:00:08Z","timestamp":1600844408000},"page":"6027","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["In-vitro study of monocytic THP-1 leukemia cell membrane elasticity with a single-cell microfluidic-assisted optical trapping system"],"prefix":"10.1364","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0933-8123","authenticated-orcid":true,"given":"Ric John L.","family":"Ombid","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glenn G.","family":"Oyong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esperanza C.","family":"Cabrera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilfred V.","family":"Espulgar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiichi","family":"Tamiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romeric F.","family":"Pobre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"285","published-online":{"date-parts":[[2020,10,1]]},"reference":[{"key":"boe-11-10-6027-R2","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1093\/abbs\/gmw081","volume":"48","author":"Quan","year":"2016","journal-title":"Acta Biochim. Biophys. Sin."},{"key":"boe-11-10-6027-R3","doi-asserted-by":"publisher","first-page":"e112582","DOI":"10.1371\/journal.pone.0112582","volume":"9","author":"Andolfi","year":"2014","journal-title":"PLoS One"},{"key":"boe-11-10-6027-R4","doi-asserted-by":"publisher","first-page":"20140028","DOI":"10.1098\/rstb.2014.0028","volume":"370","author":"Schlosser","year":"2015","journal-title":"Philos. Trans. R. Soc., B"},{"key":"boe-11-10-6027-R5","doi-asserted-by":"publisher","first-page":"24245","DOI":"10.1039\/C5RA26305K","volume":"6","author":"Fusco","year":"2016","journal-title":"RSC Adv."},{"key":"boe-11-10-6027-R6","doi-asserted-by":"publisher","first-page":"3689","DOI":"10.1529\/biophysj.104.045476","volume":"88","author":"Guck","year":"2005","journal-title":"Biophys. J."},{"key":"boe-11-10-6027-R7","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1088\/0031-9155\/58\/4\/923","volume":"58","author":"Kaffas","year":"2013","journal-title":"Phys. Med. Biol."},{"key":"boe-11-10-6027-R8","doi-asserted-by":"publisher","first-page":"9280","DOI":"10.1021\/la051837g","volume":"21","author":"Hessler","year":"2005","journal-title":"Langmuir"},{"key":"boe-11-10-6027-R9","doi-asserted-by":"publisher","first-page":"2181","DOI":"10.1016\/S0006-3495(94)81014-8","volume":"66","author":"Wang","year":"1994","journal-title":"Biophys. J."},{"key":"boe-11-10-6027-R10","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/S0021-9290(99)00175-X","volume":"33","author":"Hochmuth","year":"2000","journal-title":"J. Biomech"},{"key":"boe-11-10-6027-R12","doi-asserted-by":"publisher","first-page":"5","DOI":"10.5772\/59379","volume":"1","author":"Unal","year":"2014","journal-title":"Nanobiomedicine"},{"key":"boe-11-10-6027-R13","doi-asserted-by":"publisher","first-page":"2347","DOI":"10.3390\/molecules23092347","volume":"23","author":"Wu","year":"2018","journal-title":"Molecules"},{"key":"boe-11-10-6027-R14","doi-asserted-by":"publisher","first-page":"1449","DOI":"10.3389\/fphys.2018.01449","volume":"9","author":"Basoli","year":"2018","journal-title":"Frontiers in Physiology"},{"key":"boe-11-10-6027-R15","doi-asserted-by":"publisher","first-page":"7833","DOI":"10.1038\/s41598-017-07947-6","volume":"7","author":"Khakshour","year":"2017","journal-title":"Sci. Rep."},{"key":"boe-11-10-6027-R17","doi-asserted-by":"publisher","first-page":"1195","DOI":"10.1016\/j.biopha.2017.11.040","volume":"97","author":"Fraczkowska","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"boe-11-10-6027-R19","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1016\/S0008-6363(99)00220-5","volume":"44","author":"Galea","year":"1999","journal-title":"Cardiovasc. Res."},{"key":"boe-11-10-6027-R20","doi-asserted-by":"publisher","first-page":"276","DOI":"10.2307\/3579457","volume":"146","author":"Sylju\u00e5sen","year":"1996","journal-title":"Radiat. Res."},{"key":"boe-11-10-6027-R21","first-page":"2442","volume":"43","author":"Oshitari","year":"2002","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"boe-11-10-6027-R22","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1016\/j.devcel.2005.08.001","volume":"9","author":"Enomoto","year":"2005","journal-title":"Dev. Cell"},{"key":"boe-11-10-6027-R23","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1093\/jnci\/djs648","volume":"105","author":"Xue","year":"2013","journal-title":"J. Natl. Cancer Inst."},{"key":"boe-11-10-6027-R24","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1089\/dna.2013.2022","volume":"33","author":"Fan","year":"2014","journal-title":"DNA Cell Biol."},{"key":"boe-11-10-6027-R25","doi-asserted-by":"publisher","first-page":"e0126214","DOI":"10.1371\/journal.pone.0126214","volume":"10","author":"Jalilian","year":"2015","journal-title":"PLoS One"},{"key":"boe-11-10-6027-R26","doi-asserted-by":"publisher","first-page":"3117","DOI":"10.1016\/j.bbamcr.2015.05.008","volume":"1853","author":"Efremov","year":"2015","journal-title":"Biochim. Biophys. Acta, Mol. Cell Res."},{"key":"boe-11-10-6027-R29","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1088\/0022-3727\/31\/3\/008","volume":"31","author":"Liu","year":"1998","journal-title":"J. Phys. D: Appl. Phys."},{"key":"boe-11-10-6027-R30","author":"Johnson","year":"1985"},{"key":"boe-11-10-6027-R31","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1007\/BF01283506","volume":"9","author":"Romashin","year":"1968","journal-title":"Refractories"},{"key":"boe-11-10-6027-R32","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1146\/annurev-matsci-062910-100351","volume":"41","author":"Kollmannsberger","year":"2011","journal-title":"Annu. Rev. Mater. Res."},{"key":"boe-11-10-6027-R33","first-page":"71","article-title":"Assays related to cancer drug discovery","volume":"6","author":"SuffnessHostettmann","year":"1991"},{"key":"boe-11-10-6027-R34","first-page":"88102A","article-title":"Heat in optical tweezers","author":"del Rosal","year":"2013"},{"key":"boe-11-10-6027-R35","doi-asserted-by":"publisher","first-page":"2162","DOI":"10.1002\/smll.201201740","volume":"9","author":"Haro-Gonz\u00e1lez","year":"2013","journal-title":"Small"},{"key":"boe-11-10-6027-R36","doi-asserted-by":"publisher","first-page":"28322","DOI":"10.1074\/jbc.273.43.28322","volume":"273","author":"Tsakiridis","year":"1998","journal-title":"J. Biol. Chem."},{"key":"boe-11-10-6027-R37","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1016\/S0006-3495(00)76614-8","volume":"78","author":"Rotsch","year":"2000","journal-title":"Biophys. J."},{"key":"boe-11-10-6027-R38","doi-asserted-by":"publisher","first-page":"C521","DOI":"10.1152\/ajpcell.00429.2004","volume":"289","author":"An","year":"2005","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"boe-11-10-6027-R39","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1016\/j.bios.2009.08.011","volume":"25","author":"Wang","year":"2009","journal-title":"Biosens. Bioelectron."},{"key":"boe-11-10-6027-R40","doi-asserted-by":"publisher","first-page":"2661","DOI":"10.1091\/mbc.e17-02-0087","volume":"28","author":"Hoffman","year":"2017","journal-title":"Mol. Biol. Cell"},{"key":"boe-11-10-6027-R41","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1111\/j.1460-9568.2007.05347.x","volume":"25","author":"O\u2019Malley","year":"2007","journal-title":"EJONEI"},{"key":"boe-11-10-6027-R42","doi-asserted-by":"publisher","first-page":"e0171738","DOI":"10.1371\/journal.pone.0171738","volume":"12","author":"Tormos","year":"2017","journal-title":"PLoS One"},{"key":"boe-11-10-6027-R43","doi-asserted-by":"publisher","first-page":"3784","DOI":"10.1128\/MCB.25.9.3784-3792.2005","volume":"25","author":"Kayahara","year":"2005","journal-title":"Mol. Cell. Biol."},{"key":"boe-11-10-6027-R44","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1016\/j.bjid.2012.11.009","volume":"17","author":"Shi","year":"2013","journal-title":"Braz. J. Infect. Dis."},{"key":"boe-11-10-6027-R45","doi-asserted-by":"publisher","first-page":"1379","DOI":"10.1038\/sj.onc.1203443","volume":"19","author":"Wang","year":"2000","journal-title":"Oncogene"},{"key":"boe-11-10-6027-R46","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1016\/j.bbamcr.2006.06.006","volume":"1763","author":"Lee","year":"2006","journal-title":"Biochim. Biophys. Acta, Mol. Cell Res."},{"key":"boe-11-10-6027-R47","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1002\/wsbm.1275","volume":"6","author":"Moeendarbary","year":"2014","journal-title":"Wiley Interdiscip. Rev.: Syst. Biol. Med."},{"key":"boe-11-10-6027-R48","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1016\/j.bbrc.2010.01.107","volume":"393","author":"Yu","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."}],"container-title":["Biomedical Optics Express"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/opg.optica.org\/viewmedia.cfm?URI=boe-11-10-6027&seq=0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T19:25:14Z","timestamp":1722972314000},"score":1,"resource":{"primary":{"URL":"https:\/\/opg.optica.org\/abstract.cfm?URI=boe-11-10-6027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,1]]},"references-count":42,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020]]},"published-print":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.1364\/boe.402526","relation":{},"ISSN":["2156-7085","2156-7085"],"issn-type":[{"value":"2156-7085","type":"print"},{"value":"2156-7085","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,1]]},"assertion":[{"value":"Optica Publishing Group","name":"publisher","label":"This article is maintained by"},{"value":"https:\/\/doi.org\/10.1364\/BOE.402526","name":"articlelink","label":"Crossref DOI link to publisher maintained version"},{"value":"research-article","name":"content_type","label":"Article type"},{"value":"Screened by Similarity Check","name":"cross_check","label":"Similarity check"},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"13 July 2020","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"23 September 2020","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1 October 2020","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}},{"value":"\u00a9 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement","name":"copyright","label":"Copyright"}]}}