{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T15:13:02Z","timestamp":1778080382808,"version":"3.51.4"},"reference-count":61,"publisher":"Optica Publishing Group","issue":"12","license":[{"start":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T00:00:00Z","timestamp":1637107200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1364\/OA_License_v1#VOR-OA"},{"start":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T00:00:00Z","timestamp":1637107200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/opg.optica.org\/policies\/opg-tdm-policy.json"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21 EB021397"],"award-info":[{"award-number":["R21 EB021397"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","award":["Project 4933"],"award-info":[{"award-number":["Project 4933"]}],"id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2015-06517"],"award-info":[{"award-number":["RGPIN-2015-06517"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Eyes High International Doctoral Scholarship"},{"name":"Alberta Graduate Excellence Scholarship"}],"content-domain":{"domain":["opg.optica.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Opt. Express"],"published-print":{"date-parts":[[2021,12,1]]},"abstract":"<jats:p>We provide a protocol for measuring the absolute concentration of the oxidized and reduced state of cytochrome c oxidase (CCO) in the cerebral cortex of mice, using broadband continuous-wave NIRS. The algorithm (NIR-AQUA) allows for absolute quantification of CCO and deoxyhemoglobin. Combined with an anoxia pulse, this also allows for quantification of total hemoglobin, and tissue oxygen saturation. CCO in the cortex was 4.9\u2009\u00b1\u20090.1 \u03bcM (mean\u2009\u00b1\u2009SD, n=6). In normoxia, 84% of CCO was oxidized. We include hypoxia and cyanide validation studies to show CCO can be quantified independently to hemoglobin. This can be applied to study oxidative metabolism in the many rodent models of neurological disease.<\/jats:p>","DOI":"10.1364\/boe.435532","type":"journal-article","created":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T15:30:08Z","timestamp":1636644608000},"page":"7632","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Quantification of cytochrome c oxidase and tissue oxygenation using CW-NIRS in a mouse cerebral cortex"],"prefix":"10.1364","volume":"12","author":[{"given":"Mada","family":"Hashem","sequence":"first","affiliation":[{"name":"University of Calgary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wu","sequence":"additional","affiliation":[{"name":"University of Calgary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeff F.","family":"Dunn","sequence":"additional","affiliation":[{"name":"University of Calgary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"285","published-online":{"date-parts":[[2021,11,17]]},"reference":[{"key":"boe-12-12-7632-R1","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1203\/00006450-199601000-00005","volume":"39","author":"Cooper","year":"1996","journal-title":"Pediatr Res"},{"key":"boe-12-12-7632-R2","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1098\/rstb.1997.0046","volume":"352","author":"Delpy","year":"1997","journal-title":"Phil. Trans. R. Soc. Lond. B"},{"key":"boe-12-12-7632-R3","doi-asserted-by":"publisher","first-page":"1295","DOI":"10.1088\/0031-9155\/39\/8\/008","volume":"39","author":"Matcher","year":"1994","journal-title":"Phys. Med. Biol."},{"key":"boe-12-12-7632-R4","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1002\/iub.86","volume":"60","author":"Fontanesi","year":"2008","journal-title":"IUBMB Life"},{"key":"boe-12-12-7632-R5","doi-asserted-by":"publisher","first-page":"1264","DOI":"10.1126\/science.929199","volume":"198","author":"Jobsis","year":"1977","journal-title":"Science"},{"key":"boe-12-12-7632-R6","doi-asserted-by":"publisher","first-page":"091307","DOI":"10.1117\/1.JBO.21.9.091307","volume":"21","author":"Bale","year":"2016","journal-title":"J. Biomed. Opt"},{"key":"boe-12-12-7632-R7","doi-asserted-by":"publisher","first-page":"424","DOI":"10.3171\/JNS\/2008\/109\/9\/0424","volume":"109","author":"Tisdall","year":"2008","journal-title":"JNS"},{"key":"boe-12-12-7632-R8","doi-asserted-by":"publisher","first-page":"3450","DOI":"10.1364\/BOE.5.003450","volume":"5","author":"Bale","year":"2014","journal-title":"Biomed. Opt. Express"},{"key":"boe-12-12-7632-R9","doi-asserted-by":"publisher","first-page":"2550","DOI":"10.1364\/BOE.3.002550","volume":"3","author":"Kolyva","year":"2012","journal-title":"Biomed. Opt. Express"},{"key":"boe-12-12-7632-R10","doi-asserted-by":"publisher","first-page":"1330","DOI":"10.1038\/s41598-017-01394-z","volume":"7","author":"Siddiqui","year":"2017","journal-title":"Sci Rep"},{"key":"boe-12-12-7632-R11","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1042\/bj3320627","volume":"332","author":"Cooper","year":"1998","journal-title":"Biochem J."},{"key":"boe-12-12-7632-R12","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.neuroimage.2013.08.016","volume":"102","author":"Bainbridge","year":"2014","journal-title":"NeuroImage"},{"key":"boe-12-12-7632-R13","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1203\/00006450-199503000-00001","volume":"37","author":"Tsuji","year":"1995","journal-title":"Pediatr. Res."},{"key":"boe-12-12-7632-R14","doi-asserted-by":"publisher","first-page":"1273","DOI":"10.1213\/ANE.0000000000003572","volume":"129","author":"Thiele","year":"2019","journal-title":"Anesthesia & Analgesia"},{"key":"boe-12-12-7632-R15","doi-asserted-by":"publisher","first-page":"024002","DOI":"10.1117\/1.2718541","volume":"12","author":"Tisdall","year":"2007","journal-title":"J. Biomed. Opt."},{"key":"boe-12-12-7632-R16","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/j.neuroimage.2013.05.070","volume":"85","author":"Kolyva","year":"2014","journal-title":"NeuroImage"},{"key":"boe-12-12-7632-R17","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1006\/abio.1995.1252","volume":"227","author":"Matcher","year":"1995","journal-title":"Analytical Biochemistry"},{"key":"boe-12-12-7632-R18","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1253\/circj.CJ-16-1225","volume":"81","author":"Nosrati","year":"2017","journal-title":"Circ J"},{"key":"boe-12-12-7632-R19","doi-asserted-by":"publisher","first-page":"2761","DOI":"10.1364\/BOE.3.002761","volume":"3","author":"Yeganeh","year":"2012","journal-title":"Biomed. Opt. Express"},{"key":"boe-12-12-7632-R20","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.neuroimage.2013.05.004","volume":"85","author":"Scholkmann","year":"2014","journal-title":"NeuroImage"},{"key":"boe-12-12-7632-R21","doi-asserted-by":"publisher","first-page":"933","DOI":"10.1364\/BOE.6.000933","volume":"6","author":"Arifler","year":"2015","journal-title":"Biomed. Opt. Express"},{"key":"boe-12-12-7632-R22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1109\/JSTQE.2018.2833205","volume":"25","author":"Lange","year":"2019","journal-title":"IEEE J. Select. Topics Quantum Electron."},{"key":"boe-12-12-7632-R23","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4614-4989-8_10","article-title":"Normobaric hyperoxia does not change optical scattering or pathlength but does increase oxidised cytochrome C oxidase concentration in patients with brain injury","author":"Ghosh","year":"2013"},{"key":"boe-12-12-7632-R24","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1088\/0967-3334\/28\/9\/007","volume":"28","author":"Lee","year":"2007","journal-title":"Physiol. Meas"},{"key":"boe-12-12-7632-R25","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1016\/j.freeradbiomed.2005.08.040","volume":"40","author":"Blomgren","year":"2006","journal-title":"Free Radical Biology and Medicine"},{"key":"boe-12-12-7632-R26","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1016\/0005-2728(88)90069-2","volume":"933","author":"Wray","year":"1988","journal-title":"Biochim. Biophys. Acta"},{"key":"boe-12-12-7632-R27","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1097\/00004647-199901000-00003","volume":"19","author":"Cooper","year":"1999","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"boe-12-12-7632-R28","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.jneumeth.2010.01.037","volume":"188","author":"Zhang","year":"2010","journal-title":"Journal of Neuroscience Methods"},{"key":"boe-12-12-7632-R29","doi-asserted-by":"publisher","first-page":"116315","DOI":"10.1016\/j.neuroimage.2019.116315","volume":"206","author":"Hashem","year":"2020","journal-title":"NeuroImage"},{"key":"boe-12-12-7632-R31","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1016\/S1053-0770(96)80107-8","volume":"10","author":"Wahr","year":"1996","journal-title":"Journal of Cardiothoracic and Vascular Anesthesia"},{"key":"boe-12-12-7632-R32","doi-asserted-by":"publisher","first-page":"1433","DOI":"10.1088\/0031-9155\/33\/12\/008","volume":"33","author":"Delpy","year":"1988","journal-title":"Physics in medicine and biology"},{"key":"boe-12-12-7632-R33","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1088\/0031-9155\/39\/1\/011","volume":"39","author":"Matcher","year":"1994","journal-title":"Phys. Med. Biol."},{"key":"boe-12-12-7632-R34","doi-asserted-by":"publisher","first-page":"3381","DOI":"10.1088\/0031-9155\/55\/12\/008","volume":"55","author":"Dehghani","year":"2010","journal-title":"Phys. Med. Biol."},{"key":"boe-12-12-7632-R35","doi-asserted-by":"publisher","first-page":"1627","DOI":"10.1021\/ac60214a047","volume":"36","author":"Savitzky","year":"1964","journal-title":"Anal. Chem."},{"key":"boe-12-12-7632-R36","doi-asserted-by":"publisher","first-page":"642190","DOI":"10.1155\/2013\/642190","volume":"2013","author":"Chen","year":"2013","journal-title":"ISRN Spectroscopy"},{"key":"boe-12-12-7632-R38","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/S1056-8719(97)00053-1","volume":"38","author":"Reinoso","year":"1997","journal-title":"Journal of Pharmacological and Toxicological Methods"},{"key":"boe-12-12-7632-R39","doi-asserted-by":"publisher","first-page":"R37","DOI":"10.1088\/0031-9155\/58\/11\/R37","volume":"58","author":"Jacques","year":"2013","journal-title":"Phys. Med. Biol."},{"key":"boe-12-12-7632-R40","doi-asserted-by":"publisher","first-page":"1933","DOI":"10.1364\/BOE.3.001933","volume":"3","author":"Sakata","year":"2012","journal-title":"Biomed. Opt. Express"},{"key":"boe-12-12-7632-R41","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1088\/0967-3334\/15\/4\/006","volume":"15","author":"Skov","year":"1994","journal-title":"Physiol. Meas."},{"key":"boe-12-12-7632-R42","doi-asserted-by":"publisher","first-page":"056001","DOI":"10.1117\/1.JBO.18.5.056001","volume":"18","author":"Talukdar","year":"2013","journal-title":"J. Biomed. Opt"},{"key":"boe-12-12-7632-R43","doi-asserted-by":"publisher","first-page":"105004","DOI":"10.1117\/1.JBO.18.10.105004","volume":"18","author":"Scholkmann","year":"2013","journal-title":"J. Biomed. Opt"},{"key":"boe-12-12-7632-R44","doi-asserted-by":"publisher","first-page":"2075","DOI":"10.1088\/0031-9155\/47\/12\/306","volume":"47","author":"Zhao","year":"2002","journal-title":"Phys. Med. Biol."},{"key":"boe-12-12-7632-R45","doi-asserted-by":"crossref","DOI":"10.1117\/12.154666","article-title":"Variability of human brain and muscle optical pathlength in different experimental conditions","author":"Ferrari","year":"1993"},{"key":"boe-12-12-7632-R46","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-55231-6_10","article-title":"Evaluation of haemoglobin and cytochrome responses during forearm ischaemia using multi-wavelength time domain NIRS","author":"Lange","year":"2017"},{"key":"boe-12-12-7632-R47","author":"Wang","year":"2012"},{"key":"boe-12-12-7632-R48","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.brainres.2003.11.017","volume":"999","author":"Fujii","year":"2004","journal-title":"Brain Research"},{"key":"boe-12-12-7632-R49","doi-asserted-by":"publisher","first-page":"e43981","DOI":"10.1371\/journal.pone.0043981","volume":"7","author":"Mane","year":"2012","journal-title":"PLoS One"},{"key":"boe-12-12-7632-R50","doi-asserted-by":"publisher","first-page":"051026","DOI":"10.1117\/1.JBO.20.5.051026","volume":"20","author":"Yoshida","year":"2015","journal-title":"J. Biomed. Opt."},{"key":"boe-12-12-7632-R51","doi-asserted-by":"publisher","first-page":"491","DOI":"10.3390\/ijms19020491","volume":"19","author":"Mustari","year":"2018","journal-title":"IJMS"},{"key":"boe-12-12-7632-R52","doi-asserted-by":"publisher","first-page":"7502","DOI":"10.1038\/s41598-018-25779-w","volume":"8","author":"Vilhjalmsdottir","year":"2018","journal-title":"Sci Rep"},{"key":"boe-12-12-7632-R53","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/s0005-2728(05)80109-4","volume":"1057","author":"Brown","year":"1991","journal-title":"Biochim. Biophys. Acta"},{"key":"boe-12-12-7632-R54","doi-asserted-by":"publisher","first-page":"H2202","DOI":"10.1152\/ajpheart.2000.279.5.H2202","volume":"279","author":"Springett","year":"2000","journal-title":"American Journal of Physiology-Heart and Circulatory"},{"key":"boe-12-12-7632-R55","doi-asserted-by":"publisher","first-page":"1086","DOI":"10.1152\/jappl.1990.68.3.1086","volume":"68","author":"Wyatt","year":"1990","journal-title":"Journal of Applied Physiology"},{"key":"boe-12-12-7632-R56","doi-asserted-by":"publisher","first-page":"2753","DOI":"10.1152\/jappl.1994.77.6.2753","volume":"77","author":"Elwell","year":"1994","journal-title":"Journal of Applied Physiology"},{"key":"boe-12-12-7632-R57","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/S00723-021-01345-Y","volume":"52","author":"Hashem","year":"2021","journal-title":"Appl. Magn. Reson."},{"key":"boe-12-12-7632-R58","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1203\/00006450-199810000-00020","volume":"44","author":"Tsuji","year":"1998","journal-title":"Pediatr Res"},{"key":"boe-12-12-7632-R59","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-91287-5_24","article-title":"Broadband NIRS cerebral cytochrome-C-oxidase response to anoxia before and after hypoxic-ischaemic injury in piglets","author":"Bale","year":"2018"},{"key":"boe-12-12-7632-R60","doi-asserted-by":"publisher","first-page":"615","DOI":"10.7205\/MILMED-D-02-7408","volume":"174","author":"Lee","year":"2009","journal-title":"Military medicine"},{"key":"boe-12-12-7632-R61","doi-asserted-by":"publisher","first-page":"1224","DOI":"10.1172\/JCI111078","volume":"72","author":"Piantadosi","year":"1983","journal-title":"J. Clin. Invest."},{"key":"boe-12-12-7632-R62","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/0005-2728(72)90208-3","volume":"275","author":"Nicholls","year":"1972","journal-title":"Biochim. Biophys. Acta"},{"key":"boe-12-12-7632-R63","doi-asserted-by":"publisher","first-page":"3392","DOI":"10.1016\/S0021-9258(18)97230-3","volume":"240","author":"Yonetani","year":"1965","journal-title":"Journal of Biological Chemistry"}],"container-title":["Biomedical Optics Express"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/opg.optica.org\/viewmedia.cfm?URI=boe-12-12-7632&seq=0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T19:29:18Z","timestamp":1722972558000},"score":1,"resource":{"primary":{"URL":"https:\/\/opg.optica.org\/abstract.cfm?URI=boe-12-12-7632"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,17]]},"references-count":61,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021]]},"published-print":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.1364\/boe.435532","relation":{},"ISSN":["2156-7085","2156-7085"],"issn-type":[{"value":"2156-7085","type":"print"},{"value":"2156-7085","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,17]]},"assertion":[{"value":"Optica Publishing Group","name":"publisher","label":"This article is maintained by"},{"value":"https:\/\/doi.org\/10.1364\/BOE.435532","name":"articlelink","label":"Crossref DOI link to publisher maintained version"},{"value":"research-article","name":"content_type","label":"Article type"},{"value":"Screened by Similarity Check","name":"cross_check","label":"Similarity check"},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"1 July 2021","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"5 November 2021","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"17 November 2021","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}},{"value":"\u00a9 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement","name":"copyright","label":"Copyright"}]}}