{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T23:35:28Z","timestamp":1788824128782,"version":"build-2803163510"},"reference-count":46,"publisher":"Optica Publishing Group","issue":"11","license":[{"start":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T00:00:00Z","timestamp":1664755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1364\/OA_License_v2#VOR-OA"},{"start":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T00:00:00Z","timestamp":1664755200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/opg.optica.org\/policies\/opg-tdm-policy.json"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DBI2145235"],"award-info":[{"award-number":["DBI2145235"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["EFMA1830941"],"award-info":[{"award-number":["EFMA1830941"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01GM123517"],"award-info":[{"award-number":["R01GM123517"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM124846"],"award-info":[{"award-number":["R35GM124846"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["opg.optica.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Opt. Express"],"published-print":{"date-parts":[[2022,11,1]]},"abstract":"<jats:p>\n                    Live-cell imaging reveals the phenotypes and mechanisms of cellular function and their dysfunction that underscore cell physiology, development, and pathology. Here, we report a 3D super-resolution live-cell microscopy method by integrating radiality analysis and Fourier light-field microscopy (\n                    <jats:italic>rad-<\/jats:italic>\n                    FLFM). We demonstrated the method using various live-cell specimens, including actins in Hela cells, microtubules in mammary organoid cells, and peroxisomes in COS-7 cells. Compared with conventional wide-field microscopy,\n                    <jats:italic>rad-<\/jats:italic>\n                    FLFM realizes scanning-free, volumetric 3D live-cell imaging with sub-diffraction-limited resolution of \u223c150 nm (\n                    <jats:italic>x-y<\/jats:italic>\n                    ) and 300 nm (\n                    <jats:italic>z<\/jats:italic>\n                    ), milliseconds volume acquisition time, six-fold extended depth of focus of \u223c6 \u00b5m, and low photodamage. The method provides a promising avenue to explore spatiotemporal-challenging subcellular processes in a wide range of cell biological research.\n                  <\/jats:p>","DOI":"10.1364\/boe.471967","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T13:00:17Z","timestamp":1664283617000},"page":"5574","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["3D super-resolution live-cell imaging with radial symmetry and Fourier light-field microscopy"],"prefix":"10.1364","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5141-1673","authenticated-orcid":true,"given":"Keyi","family":"Han","sequence":"first","affiliation":[{"name":"Georgia Institute of Technology and Emory University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanwen","family":"Hua","sequence":"additional","affiliation":[{"name":"Georgia Institute of Technology and Emory University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishwa","family":"Vasani","sequence":"additional","affiliation":[{"name":"Georgia Institute of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge-Ah R.","family":"Kim","sequence":"additional","affiliation":[{"name":"Georgia Institute of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhao","family":"Liu","sequence":"additional","affiliation":[{"name":"Georgia Institute of Technology and Emory University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Takayama","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Georgia Institute of Technology and Emory University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6854-7537","authenticated-orcid":true,"given":"Shu","family":"Jia","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Georgia Institute of Technology and Emory University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2022,10,3]]},"reference":[{"key":"boe-13-11-5574-R1","doi-asserted-by":"publisher","first-page":"880","DOI":"10.1126\/science.aau1044","volume":"361","author":"Sigal","year":"2018","journal-title":"Science"},{"key":"boe-13-11-5574-R2","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1038\/nbt.3713","volume":"34","author":"Skylaki","year":"2016","journal-title":"Nat. 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Healthcare Mater."},{"key":"boe-13-11-5574-R42","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1039\/C0AN00609B","volume":"136","author":"Tung","year":"2011","journal-title":"Analyst"},{"key":"boe-13-11-5574-R43","doi-asserted-by":"publisher","first-page":"803","DOI":"10.1038\/nrm3700","volume":"14","author":"Smith","year":"2013","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"boe-13-11-5574-R44","unstructured":", \u201cAutomatic correction for sCMOS-related noise,\u201d Github, 2022, https:\/\/github.com\/ShuJiaLab\/ACsN"},{"key":"boe-13-11-5574-R45","unstructured":", \u201cHigh-resolution Fourier light-field microscopy for volumetric multi-color live-cell imaging,\u201d Github, 2022, https:\/\/github.com\/ShuJiaLab\/HR-FLFM"},{"key":"boe-13-11-5574-R46","unstructured":", \u201cSuper-resolution radial fluctuations (SRRF),\u201d Github, 2022, https:\/\/github.com\/HenriquesLab\/NanoJ-SRRF"}],"container-title":["Biomedical Optics Express"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/opg.optica.org\/viewmedia.cfm?URI=boe-13-11-5574","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/opg.optica.org\/viewmedia.cfm?URI=boe-13-11-5574&seq=0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T15:29:39Z","timestamp":1722958179000},"score":1,"resource":{"primary":{"URL":"https:\/\/opg.optica.org\/abstract.cfm?URI=boe-13-11-5574"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,3]]},"references-count":46,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022]]},"published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.1364\/boe.471967","relation":{},"ISSN":["2156-7085","2156-7085"],"issn-type":[{"value":"2156-7085","type":"print"},{"value":"2156-7085","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,3]]},"assertion":[{"value":"Optica Publishing Group","name":"publisher","label":"This article is maintained by"},{"value":"https:\/\/doi.org\/10.1364\/BOE.471967","name":"articlelink","label":"Crossref DOI link to publisher maintained version"},{"value":"research-article","name":"content_type","label":"Article type"},{"value":"Screened by Similarity Check","name":"cross_check","label":"Similarity check"},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"2 August 2022","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"26 September 2022","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"3 October 2022","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}},{"value":"\u00a9 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement","name":"copyright","label":"Copyright"}]}}