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Opt. Express"],"published-print":{"date-parts":[[2023,5,1]]},"abstract":"<jats:p>Second harmonic generation (SHG) imaging microscopy of thick biological\n\t\t\t\t\ttissues is affected by the presence of aberrations and scattering\n\t\t\t\t\twithin the sample. Moreover, additional problems, such as uncontrolled\n\t\t\t\t\tmovements, appear when imaging <jats:italic>in-vivo<\/jats:italic>. Deconvolution\n\t\t\t\t\tmethods can be used to overcome these limitations under some\n\t\t\t\t\tconditions. In particular, we present here a technique based on a\n\t\t\t\t\tmarginal blind deconvolution approach for improving SHG images\n\t\t\t\t\tobtained <jats:italic>in vivo<\/jats:italic> in the human eye (cornea and\n\t\t\t\t\tsclera). Different image quality metrics are used to quantify the\n\t\t\t\t\tattained improvement. Collagen fibers in both cornea and sclera are\n\t\t\t\t\tbetter visualized and their spatial distributions accurately assessed.\n\t\t\t\t\tThis might be a useful tool to better discriminate between healthy and\n\t\t\t\t\tpathological tissues, especially those where changes in collagen\n\t\t\t\t\tdistribution occur.<\/jats:p>","DOI":"10.1364\/boe.486989","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T03:00:06Z","timestamp":1679886006000},"page":"2117","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Blind deconvolution of second harmonic microscopy images of the living human eye"],"prefix":"10.1364","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2957-9883","authenticated-orcid":true,"given":"Rosa M.","family":"Mart\u00ednez-Ojeda","sequence":"first","affiliation":[{"name":"Instituto Universitario de Investigaci\u00f3n en \u00d3ptica y Nanof\u00edsica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8364-4957","authenticated-orcid":true,"given":"Laurent M.","family":"Mugnier","sequence":"additional","affiliation":[{"name":"ONERA \/ DOTA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1284-6591","authenticated-orcid":true,"given":"Pablo","family":"Artal","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Investigaci\u00f3n en \u00d3ptica y Nanof\u00edsica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9711-8034","authenticated-orcid":true,"given":"Juan M.","family":"Bueno","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Investigaci\u00f3n en \u00d3ptica y Nanof\u00edsica"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2023,4,19]]},"reference":[{"key":"boe-14-5-2117-R1","doi-asserted-by":"publisher","first-page":"2350","DOI":"10.1364\/AO.10.002350","volume":"10","author":"Fine","year":"1971","journal-title":"Appl. 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