{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:21:06Z","timestamp":1791840066426,"version":"4.3.4"},"reference-count":51,"publisher":"Optica Publishing Group","issue":"8","license":[{"start":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:00:00Z","timestamp":1688947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:00:00Z","timestamp":1688947200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/opg.optica.org\/policies\/opg-tdm-policy.json"}],"funder":[{"DOI":"10.13039\/501100004063","name":"Knut och Alice Wallenbergs Stiftelse","doi-asserted-by":"publisher","award":["2019-0577"],"award-info":[{"award-number":["2019-0577"]}],"id":[{"id":"10.13039\/501100004063","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2018-04900"],"award-info":[{"award-number":["2018-04900"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006552","name":"Nanoscience and Nanotechnology Area of Advance, Chalmers Tekniska H\u00f6gskola","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006552","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["opg.optica.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Opt. Express"],"published-print":{"date-parts":[[2023,8,1]]},"abstract":"<jats:p>\n                    Recent innovations in microscopy techniques are paving the way for label-free studies of single nanoscopic biological entities such as viruses, lipid-nanoparticle drug carriers, and even proteins. One such technique is waveguide evanescent-field microscopy, which offers a relatively simple, yet sensitive, way of achieving label-free light scattering-based imaging of nanoparticles on surfaces. Herein, we extend the application of this technique by incorporating microfluidic liquid control and adapting the design for use with inverted microscopes by fabricating a waveguide on a transparent substrate. We furthermore formulate analytical models describing scattering and fluorescence intensities from single spherical and shell-like objects interacting with evanescent fields. The models are then applied to analyze scattering and fluorescence intensities from adsorbed polystyrene beads and to temporally resolve cholera-toxin B (CTB) binding to individual surface-immobilized glycosphingolipid G\n                    <jats:sub>M1<\/jats:sub>\n                    containing vesicles. We also propose a self-consistent means to quantify the thickness of the CTB layer, revealing that protein-binding to individual vesicles can be characterized with sub-nm precision in a time-resolved manner.\n                  <\/jats:p>","DOI":"10.1364\/boe.490051","type":"journal-article","created":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T02:18:35Z","timestamp":1687486715000},"page":"4003","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Label-free quantification of protein binding to lipid vesicles using transparent waveguide evanescent-field scattering microscopy with liquid control"],"prefix":"10.1364","volume":"14","author":[{"given":"Mokhtar","family":"Mapar","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040wg7k59","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattias","family":"Sj\u00f6berg","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040wg7k59","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Nanolyze AB, BioVentureHub"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir P.","family":"Zhdanov","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040wg7k59","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Russian Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3364-7196","authenticated-orcid":true,"given":"Bj\u00f6rn","family":"Agnarsson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040wg7k59","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Nanolyze AB, BioVentureHub"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1994-5015","authenticated-orcid":true,"given":"Fredrik","family":"H\u00f6\u00f6k","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040wg7k59","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2023,7,10]]},"reference":[{"key":"boe-14-8-4003-R1","doi-asserted-by":"publisher","first-page":"1676","DOI":"10.1126\/science.283.5408.1676","volume":"283","author":"Weiss","year":"1999","journal-title":"Science"},{"key":"boe-14-8-4003-R2","doi-asserted-by":"publisher","first-page":"1258","DOI":"10.1021\/ac3027178","volume":"85","author":"Walt","year":"2013","journal-title":"Anal. 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Further distribution of this work must maintain attribution to the author(s) and the published article\u2019s title, journal citation, and DOI.","name":"copyright","label":"Copyright"}]}}