{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:27:41Z","timestamp":1785432461206,"version":"3.56.0"},"reference-count":45,"publisher":"Optica Publishing Group","issue":"7","license":[{"start":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T00:00:00Z","timestamp":1686614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T00:00:00Z","timestamp":1686614400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/opg.optica.org\/policies\/opg-tdm-policy.json"}],"funder":[{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/P030017\/1"],"award-info":[{"award-number":["EP\/P030017\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/R004854\/1"],"award-info":[{"award-number":["EP\/R004854\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["FL210100099"],"award-info":[{"award-number":["FL210100099"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP2003786"],"award-info":[{"award-number":["APP2003786"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Future Making Fellowship"},{"DOI":"10.13039\/100009727","name":"Hospital Research Foundation","doi-asserted-by":"publisher","award":["Mid-career fellowship C-MCF58-2019"],"award-info":[{"award-number":["Mid-career fellowship C-MCF58-2019"]}],"id":[{"id":"10.13039\/100009727","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["opg.optica.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Opt. Express"],"published-print":{"date-parts":[[2023,7,1]]},"abstract":"<jats:p>\n                    Embryo quality is a crucial factor affecting live birth outcomes. However, an accurate diagnostic for embryo quality remains elusive in the\n                    <jats:italic>in vitro<\/jats:italic>\n                    fertilization clinic. Determining physical parameters of the embryo may offer key information for this purpose. Here, we demonstrate that digital holographic microscopy (DHM) can rapidly and non-invasively assess the refractive index of mouse embryos. Murine embryos were cultured in either low- or high-lipid containing media and digital holograms recorded at various stages of development. The phase of the recorded hologram was numerically retrieved, from which the refractive index of the embryo was calculated. We showed that DHM can detect spatio-temporal changes in refractive index during embryo development that are reflective of its lipid content. As accumulation of intracellular lipid is known to compromise embryo health, DHM may prove beneficial in developing an accurate, non-invasive, multimodal diagnostic.\n                  <\/jats:p>","DOI":"10.1364\/boe.492292","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T04:03:39Z","timestamp":1685505819000},"page":"3327","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Investigation of refractive index dynamics during\n                    <i>in vitro<\/i>\n                    embryo development using off-axis digital holographic microscopy"],"prefix":"10.1364","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2475-4969","authenticated-orcid":true,"given":"George O.","family":"Dwapanyin","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02wn5qz54","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darren J. X.","family":"Chow","sequence":"additional","affiliation":[{"name":"The University of Adelaide"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany C. Y.","family":"Tan","sequence":"additional","affiliation":[{"name":"The University of Adelaide"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3542-7760","authenticated-orcid":true,"given":"Nicolas S.","family":"Dubost","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02wn5qz54","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine M.","family":"Morizet","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02wn5qz54","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kylie R.","family":"Dunning","sequence":"additional","affiliation":[{"name":"The University of Adelaide"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6534-9009","authenticated-orcid":true,"given":"Kishan","family":"Dholakia","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02wn5qz54","id-type":"ROR","asserted-by":"publisher"}]},{"name":"The University of Adelaide"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2023,6,13]]},"reference":[{"key":"boe-14-7-3327-R1","doi-asserted-by":"publisher","first-page":"dev190629","DOI":"10.1242\/dev.190629","volume":"147","author":"Shahbazi","year":"2020","journal-title":"Development"},{"key":"boe-14-7-3327-R2","doi-asserted-by":"publisher","first-page":"S430","DOI":"10.1016\/j.fertnstert.2008.07.1268","volume":"90","author":"Patanayak","year":"2008","journal-title":"Fertil. Steril."},{"key":"boe-14-7-3327-R3","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1093\/humupd\/dmq015","volume":"16","author":"van Loendersloot","year":"2010","journal-title":"Hum. Reprod. Update"},{"key":"boe-14-7-3327-R4","doi-asserted-by":"publisher","first-page":"2832","DOI":"10.1093\/humrep\/deaa250","volume":"35","author":"De Geyter","year":"2020","journal-title":"Hum. Reprod."},{"key":"boe-14-7-3327-R5","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1071\/RD22217","volume":"35","author":"Tan","year":"2022","journal-title":"Reprod. Fertil. Dev."},{"key":"boe-14-7-3327-R6","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/j.fertnstert.2019.03.033","volume":"112","author":"Jung","year":"2019","journal-title":"Fertil. Steril."},{"key":"boe-14-7-3327-R7","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1093\/humrep\/deab233","volume":"37","author":"Tan","year":"2021","journal-title":"Hum. Reprod."},{"key":"boe-14-7-3327-R8","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1093\/humrep\/deac016","volume":"37","author":"Shah","year":"2022","journal-title":"Hum. Reprod."},{"key":"boe-14-7-3327-R9","doi-asserted-by":"publisher","first-page":"4165","DOI":"10.1038\/s41598-017-04220-8","volume":"7","author":"Karnowski","year":"2017","journal-title":"Sci. Rep."},{"key":"boe-14-7-3327-R10","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1530\/REP-22-0178","volume":"164","author":"Fluks","year":"2022","journal-title":"Reproduction"},{"key":"boe-14-7-3327-R11","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1364\/BOE.6.000963","volume":"6","author":"An","year":"2015","journal-title":"Biomed. Opt. Express"},{"key":"boe-14-7-3327-R12","doi-asserted-by":"publisher","first-page":"A222","DOI":"10.1364\/AO.404298","volume":"60","author":"Li","year":"2021","journal-title":"Appl. Opt."},{"key":"boe-14-7-3327-R13","first-page":"614","article-title":"Properties of Optical and Laser-Related Materials: A Handbook","author":"Nikogosyan","year":"2003"},{"key":"boe-14-7-3327-R14","doi-asserted-by":"publisher","first-page":"6524","DOI":"10.1038\/s41598-018-24393-0","volume":"8","author":"Jung","year":"2018","journal-title":"Sci. Rep."},{"key":"boe-14-7-3327-R15","doi-asserted-by":"publisher","first-page":"1438","DOI":"10.1016\/j.fertnstert.2012.02.034","volume":"97","author":"Yang","year":"2012","journal-title":"Fertil. Steril."},{"key":"boe-14-7-3327-R16","doi-asserted-by":"publisher","first-page":"2371","DOI":"10.1007\/s10815-021-02255-5","volume":"38","author":"Zander-Fox","year":"2021","journal-title":"J. Assist. Reprod. Genet."},{"key":"boe-14-7-3327-R17","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1002\/mrd.1131","volume":"61","author":"Abe","year":"2002","journal-title":"Mol. Reprod. 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Genet."},{"key":"boe-14-7-3327-R28","doi-asserted-by":"publisher","first-page":"e0260123","DOI":"10.1371\/journal.pone.0260123","volume":"16","author":"Soto-Moreno","year":"2021","journal-title":"PLoS One"},{"key":"boe-14-7-3327-R29","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1364\/AOP.6.000057","volume":"6","author":"Bhaduri","year":"2014","journal-title":"Adv. Opt. Photonics"},{"key":"boe-14-7-3327-R30","first-page":"018005","volume":"1","author":"Kim","year":"2010","journal-title":"SPIE Reviews"},{"key":"boe-14-7-3327-R31","first-page":"151","article-title":"Determination of the integral refractive index of cells in suspension by digital holographic phase contrast microscopy","author":"Kosmeier","year":"2008"},{"key":"boe-14-7-3327-R32","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1364\/JOSA.72.000156","volume":"72","author":"Takeda","year":"1982","journal-title":"J. Opt. Soc. 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Opt."},{"key":"boe-14-7-3327-R38","doi-asserted-by":"publisher","first-page":"7079","DOI":"10.1364\/AO.56.007079","volume":"56","author":"Martinez-Carranza","year":"2017","journal-title":"Appl. Opt."},{"key":"boe-14-7-3327-R39","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1364\/OL.42.000227","volume":"42","author":"Min","year":"2017","journal-title":"Opt. Lett."},{"key":"boe-14-7-3327-R40","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1002\/cyto.a.24229","volume":"99","author":"Baczewska","year":"2021","journal-title":"Cytometry"},{"key":"boe-14-7-3327-R41","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1016\/j.jlumin.2018.09.017","volume":"205","author":"Zhang","year":"2019","journal-title":"J. Lumin."},{"key":"boe-14-7-3327-R42","doi-asserted-by":"publisher","first-page":"6636","DOI":"10.1038\/s41598-019-42958-5","volume":"9","author":"Perevedentseva","year":"2019","journal-title":"Sci. Rep."},{"key":"boe-14-7-3327-R43","doi-asserted-by":"publisher","first-page":"43631","DOI":"10.1038\/srep43631","volume":"7","author":"McReynolds","year":"2017","journal-title":"Sci. Rep."},{"key":"boe-14-7-3327-R44","doi-asserted-by":"publisher","first-page":"13706","DOI":"10.1364\/OE.27.013706","volume":"27","author":"Gupta","year":"2019","journal-title":"Opt. Express"},{"key":"boe-14-7-3327-R45","doi-asserted-by":"publisher","author":"Dwapanyin","year":"2023","DOI":"10.17630\/87a7bc38-ddc6-4f65-a403-8be7297293ac"}],"container-title":["Biomedical Optics Express"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/opg.optica.org\/viewmedia.cfm?URI=boe-14-7-3327&seq=0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T15:33:29Z","timestamp":1722958409000},"score":1,"resource":{"primary":{"URL":"https:\/\/opg.optica.org\/abstract.cfm?URI=boe-14-7-3327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,13]]},"references-count":45,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023]]},"published-print":{"date-parts":[[2023]]}},"URL":"https:\/\/doi.org\/10.1364\/boe.492292","relation":{},"ISSN":["2156-7085","2156-7085"],"issn-type":[{"value":"2156-7085","type":"print"},{"value":"2156-7085","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,13]]},"assertion":[{"value":"Optica Publishing Group","name":"publisher","label":"This article is maintained by"},{"value":"https:\/\/doi.org\/10.1364\/BOE.492292","name":"articlelink","label":"Crossref DOI link to publisher maintained version"},{"value":"research-article","name":"content_type","label":"Article type"},{"value":"Screened by Similarity Check","name":"cross_check","label":"Similarity check"},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"18 April 2023","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"25 May 2023","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"13 June 2023","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}},{"value":"Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article\u2019s title, journal citation, and DOI.","name":"copyright","label":"Copyright"}]}}