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Opt. Express"],"published-print":{"date-parts":[[2024,6,1]]},"abstract":"<jats:p>\n                    Molecular specificity in fluorescence imaging of cells and tissues can be increased by measuring parameters other than intensity. For instance, fluorescence lifetime imaging became a widespread modality for biomedical optics. Previously, we suggested using the fluorescence saturation effect at pulsed laser excitation to map the absorption cross-section as an additional molecular contrast in two-photon microscopy [\n                    <jats:mixed-citation publication-type=\"journal\">\n                      <jats:source>Opt. Lett.<\/jats:source>\n                      <jats:volume>47<\/jats:volume>\n                      (\n                      <jats:issue>17<\/jats:issue>\n                      ),\n                      <jats:fpage>4455<\/jats:fpage>\n                      (\n                      <jats:year>2022<\/jats:year>\n                      ).\n                      <jats:pub-id pub-id-type=\"doi\">10.1364\/OL.465605<\/jats:pub-id>\n                    <\/jats:mixed-citation>\n                    ]. Here, it is shown that, somewhat counterintuitive, fluorescence saturation can be observed under cw excitation in a standard confocal microscopy setup. Mapping the fluorescence saturation parameter allows obtaining additional information about the fluorophores in the system, as demonstrated by the example of peptide hydrogel, stained cells and unstained thyroid gland. The suggested technique does not require additional equipment and can be implemented on confocal systems as is.\n                  <\/jats:p>","DOI":"10.1364\/boe.512188","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T03:00:06Z","timestamp":1708484406000},"page":"3755","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fluorescence saturation imaging microscopy: molecular fingerprinting with a standard confocal microscope"],"prefix":"10.1364","volume":"15","author":[{"given":"Boris","family":"Yakimov","sequence":"first","affiliation":[{"name":"Sechenov First Moscow State Medical University"},{"name":"Vorohobov\u2019s City Clinical Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Rovnyagina","sequence":"additional","affiliation":[{"name":"Sechenov First Moscow State Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afraa","family":"Hasan","sequence":"additional","affiliation":[{"name":"HSE University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juntao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wuhan Polytechnic University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuhan Polytechnic University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Fadeev","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/010pmpe69","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliya","family":"Urusova","sequence":"additional","affiliation":[{"name":"Endocrinology Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeny","family":"Khoroshilov","sequence":"additional","affiliation":[{"name":"P.N. 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