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Opt. Express"],"published-print":{"date-parts":[[2025,8,1]]},"abstract":"<jats:p>Adaptive optics-optical coherence tomography (AO-OCT) enables cellular-level in vivo visualization of cone photoreceptors in the human retina. Cone biomarkers, such as density, inner segment (IS), and outer segment (OS) lengths, are potentially important for the early detection of many outer retinal conditions. However, their dense spatial packing necessitates automated analytical methods, and most existing approaches focus primarily on cone detection without addressing their detailed structural characteristics. To address this limitation, a unified neural network, termed ISOSNet, is introduced for simultaneous cone detection and IS\/OS length measurement. Labeled AO-OCT B-scan datasets, encompassing healthy individuals across multiple retinal locations, were collected for model training and evaluation. Experimental results demonstrate an F1 score of 0.886 for cone detection and relative error rates of 6% and 11% for IS and OS length measurement, respectively. Validation on images from diseased retinas\u2014despite the model being trained only on healthy retina data\u2014highlights the generalizability of the proposed framework.<\/jats:p>","DOI":"10.1364\/boe.563128","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T08:59:59Z","timestamp":1747904399000},"page":"3237","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["ISOSNet: a unified framework for cone photoreceptor detection and inner segment and outer segment length measurement from AO-OCT B-scans"],"prefix":"10.1364","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8259-5874","authenticated-orcid":true,"given":"Mengxi","family":"Zhou","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00rs6vg23","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9337-6839","authenticated-orcid":true,"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00rs6vg23","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eli","family":"Kirkendall","sequence":"additional","affiliation":[{"name":"The Ohio State University, College of Optometry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amin","family":"Karimi Monsefi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00rs6vg23","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Wolfe","sequence":"additional","affiliation":[{"name":"Havener Eye Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiran A.","family":"Chitkara","sequence":"additional","affiliation":[{"name":"Havener Eye Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey S.","family":"Choi","sequence":"additional","affiliation":[{"name":"The Ohio State University, College of Optometry"},{"name":"The Ohio State University, College of Arts and Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Doble","sequence":"additional","affiliation":[{"name":"The Ohio State University, College of Optometry"},{"name":"The Ohio State University, College of Arts and Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srinivasan","family":"Parthasarathy","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00rs6vg23","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajiv","family":"Ramnath","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00rs6vg23","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2025,7,17]]},"reference":[{"key":"boe-16-8-3237-R1","first-page":"1236","volume":"37","author":"Curcio","year":"1996","journal-title":"Investigative Ophthalmology Visual Science"},{"key":"boe-16-8-3237-R2","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1001\/archophthalmol.2008.622","volume":"127","author":"Shelley","year":"2009","journal-title":"Arch. 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