{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:42:45Z","timestamp":1785426165554,"version":"3.56.0"},"reference-count":27,"publisher":"Optica Publishing Group","issue":"10","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1364\/OA_License_v2#VOR-OA"},{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/opg.optica.org\/policies\/opg-tdm-policy.json"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2024-00339900"],"award-info":[{"award-number":["RS-2024-00339900"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2024-00406852"],"award-info":[{"award-number":["RS-2024-00406852"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2023-00280255"],"award-info":[{"award-number":["RS-2023-00280255"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019943","name":"Korea Toray Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019943","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["opg.optica.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Opt. Express"],"published-print":{"date-parts":[[2025,10,1]]},"abstract":"<jats:p>Stochastic optical reconstruction microscopy (STORM) relies on controlled photoswitching of fluorophores, enabled by specialized imaging buffers containing thiols and oxygen scavengers. The glucose oxidase and catalase (GLOX) system is still the most widely used, especially for Alexa Fluor 647 dyes. However, GLOX gradually acidifies the buffer due to oxygen infiltration, and this pH drop degrades single-molecule blinking and limits imaging duration to several hours even when sealed with conventional materials like nail polish. While alternative pH-stable scavenger systems have been developed, sealing strategies themselves remain an underexplored yet critical factor affecting buffer longevity and imaging quality. Here, we introduce ultraviolet (UV) curable resin as a novel and effective sealant for STORM. Inspired by its widespread use in electronic device encapsulation, we demonstrate that UV resin provides rapid, air-tight sealing within seconds and outperforms traditional sealants, including nail polish, CytoSeal 60, and 5-minute epoxy, when applied to GLOX-based buffers. Using COS-7 cells labeled with Alexa Fluor 647 and CF568, we show that UV resin preserves key single-molecule and image quality metrics for at least 48 h post-sealing. Inexpensive, solvent-free, odorless, and rapidly curable, UV resin offers a highly practical sealing option that significantly extends imaging duration and enhances workflow efficiency in STORM experiments.<\/jats:p>","DOI":"10.1364\/boe.574425","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T08:00:09Z","timestamp":1756195209000},"page":"3884","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UV curable resin as a rapid and superior sealant for STORM"],"prefix":"10.1364","volume":"16","author":[{"given":"Youngseop","family":"Lee","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5721-4809","authenticated-orcid":true,"given":"Minchol","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoonhee","family":"Park","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changsoon","family":"Kim","sequence":"additional","affiliation":[{"name":"Seoul National University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1235-1487","authenticated-orcid":true,"given":"Jeongmin","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Advanced Institute of Convergence Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"boe-16-10-3884-R1","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1038\/nprot.2011.336","volume":"6","author":"van de Linde","year":"2011","journal-title":"Nat. Protoc."},{"key":"boe-16-10-3884-R2","doi-asserted-by":"publisher","first-page":"6364","DOI":"10.1021\/nn301895c","volume":"6","author":"Swoboda","year":"2012","journal-title":"ACS Nano"},{"key":"boe-16-10-3884-R3","doi-asserted-by":"publisher","first-page":"1826","DOI":"10.1529\/biophysj.107.117689","volume":"94","author":"Aitken","year":"2008","journal-title":"Biophys. J."},{"key":"boe-16-10-3884-R4","doi-asserted-by":"publisher","first-page":"e0158884","DOI":"10.1371\/journal.pone.0158884","volume":"11","author":"Nahidiazar","year":"2016","journal-title":"PLoS One"},{"key":"boe-16-10-3884-R5","doi-asserted-by":"publisher","first-page":"3926","DOI":"10.1021\/acsphotonics.2c01249","volume":"9","author":"Abdelsayed","year":"2022","journal-title":"ACS Photonics"},{"key":"boe-16-10-3884-R6","doi-asserted-by":"publisher","first-page":"2589","DOI":"10.1021\/acsphotonics.3c00806","volume":"10","author":"Lee","year":"2023","journal-title":"ACS Photonics"},{"key":"boe-16-10-3884-R7","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1016\/j.neuron.2010.11.021","volume":"68","author":"Dani","year":"2010","journal-title":"Neuron"},{"key":"boe-16-10-3884-R8","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/bs.mcb.2014.10.004","volume":"125","author":"Pereira","year":"2015","journal-title":"Methods Cell Biol."},{"key":"boe-16-10-3884-R9","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1083\/jcb.201407063","volume":"207","author":"Lorenzo","year":"2014","journal-title":"J. Cell Biol."},{"key":"boe-16-10-3884-R10","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1038\/cr.2015.115","volume":"25","author":"Wang","year":"2015","journal-title":"Cell Res."},{"key":"boe-16-10-3884-R11","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1091\/mbc.E21-11-0589","volume":"33","author":"Kaplan","year":"2022","journal-title":"Mol. Biol. Cell"},{"key":"boe-16-10-3884-R12","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1038\/nmeth.1208","volume":"5","author":"Roy","year":"2008","journal-title":"Nat. Methods"},{"key":"boe-16-10-3884-R13","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/2192-2853-1-6","volume":"1","author":"Babcock","year":"2012","journal-title":"Opt. Nanoscopy"},{"key":"boe-16-10-3884-R14","doi-asserted-by":"publisher","first-page":"100971","DOI":"10.1016\/j.xpro.2021.100971","volume":"2","author":"Vatan","year":"2021","journal-title":"STAR Protoc."},{"key":"boe-16-10-3884-R15","doi-asserted-by":"publisher","first-page":"14635","DOI":"10.1073\/pnas.1516928112","volume":"112","author":"Prakash","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"boe-16-10-3884-R16","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1038\/s42003-020-0908-2","volume":"3","author":"Liu","year":"2020","journal-title":"Commun. Biol."},{"key":"boe-16-10-3884-R17","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1111\/nan.12646","volume":"47","author":"Codron","year":"2021","journal-title":"Neuropathol. Appl. Neurobiol."},{"key":"boe-16-10-3884-R18","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1109\/TADVP.2006.890150","volume":"30","author":"Wang","year":"2007","journal-title":"IEEE Trans. Adv. Packag."},{"key":"boe-16-10-3884-R20","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.solmat.2013.05.013","volume":"117","author":"Roesch","year":"2013","journal-title":"Sol. Energy Mater. Sol. Cells"},{"key":"boe-16-10-3884-R21","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.solmat.2012.12.041","volume":"111","author":"Lee","year":"2013","journal-title":"Sol. Energy Mater. Sol. Cells"},{"key":"boe-16-10-3884-R22","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1108\/eb043595","volume":"5","author":"Hickman","year":"1979","journal-title":"Circuit World"},{"key":"boe-16-10-3884-R23","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1038\/nmeth.4661","volume":"15","author":"Li","year":"2018","journal-title":"Nat. Methods"},{"key":"boe-16-10-3884-R24","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1038\/s41377-023-01321-0","volume":"12","author":"Zhao","year":"2023","journal-title":"Light Sci. Appl."},{"key":"boe-16-10-3884-R25","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1016\/j.dental.2014.02.021","volume":"30","author":"Wydra","year":"2014","journal-title":"Dent. Mater."},{"key":"boe-16-10-3884-R26","doi-asserted-by":"publisher","author":"Lee","year":"2025","DOI":"10.6084\/m9.figshare.29963933"},{"key":"boe-16-10-3884-R27","doi-asserted-by":"publisher","first-page":"e69004","DOI":"10.1371\/journal.pone.0069004","volume":"8","author":"Olivier","year":"2013","journal-title":"PLoS One"},{"key":"boe-16-10-3884-R28","doi-asserted-by":"publisher","first-page":"732","DOI":"10.1021\/acs.jpcb.2c06872","volume":"127","author":"Herdly","year":"2023","journal-title":"J. Phys. Chem. B"}],"container-title":["Biomedical Optics Express"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/opg.optica.org\/viewmedia.cfm?URI=boe-16-10-3884&seq=0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T18:13:05Z","timestamp":1756836785000},"score":1,"resource":{"primary":{"URL":"https:\/\/opg.optica.org\/abstract.cfm?URI=boe-16-10-3884"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,2]]},"references-count":27,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025]]},"published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.1364\/boe.574425","relation":{},"ISSN":["2156-7085","2156-7085"],"issn-type":[{"value":"2156-7085","type":"print"},{"value":"2156-7085","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,2]]},"assertion":[{"value":"Optica Publishing Group","name":"publisher","label":"This article is maintained by"},{"value":"https:\/\/doi.org\/10.1364\/BOE.574425","name":"articlelink","label":"Crossref DOI link to publisher maintained version"},{"value":"research-article","name":"content_type","label":"Article type"},{"value":"Screened by Similarity Check","name":"cross_check","label":"Similarity check"},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"25 July 2025","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"25 August 2025","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2 September 2025","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}},{"value":"\u00a9 2025 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement","name":"copyright","label":"Copyright"}]}}