{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:28:19Z","timestamp":1785432499535,"version":"3.56.0"},"reference-count":37,"publisher":"Optica Publishing Group","issue":"6","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1364\/OA_License_v2#VOR-OA"},{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/opg.optica.org\/policies\/opg-tdm-policy.json"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["CDA941290"],"award-info":[{"award-number":["CDA941290"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"},{"id":"https:\/\/ror.org\/013kjyp64","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["25IPA1456700"],"award-info":[{"award-number":["25IPA1456700"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"},{"id":"https:\/\/ror.org\/013kjyp64","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["24TPA1299954"],"award-info":[{"award-number":["24TPA1299954"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"},{"id":"https:\/\/ror.org\/013kjyp64","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["25POST1377493"],"award-info":[{"award-number":["25POST1377493"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"},{"id":"https:\/\/ror.org\/013kjyp64","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["26PRE1560116"],"award-info":[{"award-number":["26PRE1560116"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"},{"id":"https:\/\/ror.org\/013kjyp64","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"crossref","award":["R03AG070479"],"award-info":[{"award-number":["R03AG070479"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"crossref"},{"id":"https:\/\/ror.org\/049v75w11","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"crossref","award":["R21 AG080775"],"award-info":[{"award-number":["R21 AG080775"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"crossref"},{"id":"https:\/\/ror.org\/049v75w11","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"crossref","award":["K01AG073614"],"award-info":[{"award-number":["K01AG073614"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"crossref"},{"id":"https:\/\/ror.org\/049v75w11","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"crossref","award":["R01AG070915"],"award-info":[{"award-number":["R01AG070915"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"crossref"},{"id":"https:\/\/ror.org\/049v75w11","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":["opg.optica.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Opt. Express"],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:p>\n                    Multi-contrast optical coherence tomography (MC-OCT), incorporating optical coherence tomography angiography (OCTA) and polarization-sensitive OCT (PS-OCT), offers a powerful non-invasive approach for interrogating ovarian microvascular and extracellular matrix (ECM) remodeling\n                    <jats:italic>in vivo<\/jats:italic>\n                    . However, its application to ovarian aging has remained largely unexplored. Here, we establish and validate MC-OCT as a robust imaging platform for visualizing and quantifying age-related changes in vascular architecture and collagen organization in mouse ovaries. Using\n                    <jats:italic>in vivo<\/jats:italic>\n                    OCTA, we demonstrate a progressive decline in ovarian microvascular density, complexity, and connectivity with advancing age. Complementary PS-OCT imaging reveals increased collagen-associated birefringence, indicative of fibrotic ECM remodeling. Quantitative OCT-derived metrics show strong agreement with histological validation using immunohistochemistry, second harmonic generation microscopy, and Sirius Red staining, confirming the accuracy and reproducibility of MC-OCT measurements. Importantly, MC-OCT enables longitudinal, real-time assessment of ovarian structure without tissue disruption, allowing detection of age-dependent remodeling that is not accessible with conventional\n                    <jats:italic>ex vivo<\/jats:italic>\n                    methods. Together, these findings establish MC-OCT as a sensitive and reliable tool for non-destructive evaluation of ovarian vascular and fibrotic changes, providing a valuable platform for studying ovarian aging and assessing interventions aimed at preserving reproductive health.\n                  <\/jats:p>","DOI":"10.1364\/boe.593302","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T02:00:09Z","timestamp":1776909609000},"page":"2897","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi-contrast optical coherence tomography for\n                    <i>in vivo<\/i>\n                    visualization and quantification of vascular features and collagen in mouse ovaries in aging"],"prefix":"10.1364","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4016-3384","authenticated-orcid":true,"given":"Sharon","family":"Negri","sequence":"first","affiliation":[{"name":"University of Oklahoma Health Sciences Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9926-7554","authenticated-orcid":true,"given":"Feng","family":"Yan","sequence":"additional","affiliation":[{"name":"University of Oklahoma"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madison","family":"Milan","sequence":"additional","affiliation":[{"name":"University of Oklahoma Health Sciences Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafal","family":"Gulej","sequence":"additional","affiliation":[{"name":"University of Oklahoma Health Sciences Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Ihuoma","sequence":"additional","affiliation":[{"name":"University of Oklahoma Health Sciences Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Kosmider","sequence":"additional","affiliation":[{"name":"University of Oklahoma Health Sciences Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherwin","family":"Tavakol","sequence":"additional","affiliation":[{"name":"University of Oklahoma Health Sciences Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Troyano-Rodriguez","sequence":"additional","affiliation":[{"name":"University of Oklahoma Health Sciences Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh","family":"Rudraboina","sequence":"additional","affiliation":[{"name":"University of Oklahoma Health Sciences Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"University of Oklahoma"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Csiszar","sequence":"additional","affiliation":[{"name":"University of Oklahoma Health Sciences Center"},{"name":"Semmelweis University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9499-5384","authenticated-orcid":true,"given":"Qinggong","family":"Tang","sequence":"additional","affiliation":[{"name":"University of Oklahoma"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Tarantini","sequence":"additional","affiliation":[{"name":"University of Oklahoma Health Sciences Center"},{"name":"Semmelweis University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2026,5,11]]},"reference":[{"key":"boe-17-6-2897-R1","doi-asserted-by":"publisher","first-page":"5103","DOI":"10.1007\/s11357-024-01139-7","type":"journal-article","volume":"46","author":"Csiszar","year":"2024","journal-title":"Geroscience"},{"key":"boe-17-6-2897-R2","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1186\/s13048-023-01300-4","type":"journal-article","volume":"16","author":"Shen","year":"2023","journal-title":"J. Ovarian Res."},{"key":"boe-17-6-2897-R3","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1095\/biolreprod32.5.989","type":"journal-article","volume":"32","author":"Parkening","year":"1985","journal-title":"Biol. Reprod."},{"key":"boe-17-6-2897-R4","first-page":"111","type":"journal-article","volume":"5","author":"Kaczmarek","year":"2005","journal-title":"Reprod. Biol."},{"key":"boe-17-6-2897-R5","doi-asserted-by":"publisher","first-page":"e13259","DOI":"10.1111\/acel.13259","type":"journal-article","volume":"19","author":"Amargant","year":"2020","journal-title":"Aging Cell."},{"key":"boe-17-6-2897-R6","doi-asserted-by":"publisher","first-page":"eabi8683","DOI":"10.1126\/sciadv.abi8683","type":"journal-article","volume":"8","author":"Xu","year":"2022","journal-title":"Sci. Adv."},{"key":"boe-17-6-2897-R7","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1148\/rg.2021200086","type":"journal-article","volume":"41","author":"Taylor","year":"2021","journal-title":"Radiographics"},{"key":"boe-17-6-2897-R8","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1053\/j.sult.2019.04.002","type":"journal-article","volume":"40","author":"Shetty","year":"2019","journal-title":"Semin. Ultrasound CT MR"},{"key":"boe-17-6-2897-R10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1117\/1.JBO.24.9.096010","type":"journal-article","volume":"24","author":"Sawyer","year":"2019","journal-title":"J. Biomed. 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Lett."},{"key":"boe-17-6-2897-R23","doi-asserted-by":"publisher","first-page":"3224","DOI":"10.1021\/ac1032325","type":"journal-article","volume":"83","author":"Campagnola","year":"2011","journal-title":"Anal. Chem."},{"key":"boe-17-6-2897-R24","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1038\/nprot.2012.009","type":"journal-article","volume":"7","author":"Chen","year":"2012","journal-title":"Nat. Protoc."},{"key":"boe-17-6-2897-R26","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1007\/s11357-023-00990-4","type":"journal-article","volume":"46","author":"Kiss","year":"2023","journal-title":"Geroscience"},{"key":"boe-17-6-2897-R27","doi-asserted-by":"publisher","first-page":"e202300409","DOI":"10.1002\/jbio.202300409","type":"journal-article","volume":"17","author":"Yan","year":"2024","journal-title":"J. Biophotonics"},{"key":"boe-17-6-2897-R28","doi-asserted-by":"publisher","first-page":"1499","DOI":"10.1007\/s11357-023-00909-z","type":"journal-article","volume":"46","author":"Venz","year":"2023","journal-title":"Geroscience"},{"key":"boe-17-6-2897-R29","doi-asserted-by":"publisher","first-page":"614","DOI":"10.1093\/humupd\/dmae020","type":"journal-article","volume":"30","author":"Guo","year":"2024","journal-title":"Hum. Reprod. 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Genet."},{"key":"boe-17-6-2897-R37","doi-asserted-by":"publisher","first-page":"2139","DOI":"10.1007\/s11357-023-00979-z","type":"journal-article","volume":"46","author":"\u00c1vila","year":"2023","journal-title":"Geroscience"},{"key":"boe-17-6-2897-R38","doi-asserted-by":"publisher","first-page":"3445","DOI":"10.1007\/s11357-024-01089-0","type":"journal-article","volume":"46","author":"Garcia","year":"2024","journal-title":"Geroscience"},{"key":"boe-17-6-2897-R39","doi-asserted-by":"publisher","first-page":"3061","DOI":"10.1007\/s11357-024-01465-w","type":"journal-article","volume":"47","author":"Isola","year":"2024","journal-title":"Geroscience"},{"key":"boe-17-6-2897-R40","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1515\/mr-2022-0031","type":"journal-article","volume":"2","author":"Zhu","year":"2023","journal-title":"Med. 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