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Opt. Express"],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:p>\n                    Laser capture microdissection (LCM) enables spatially selective isolation of cells and tissues, but precise cutting in thick biological samples is constrained by the limited axial interaction range of Gaussian beams and the side-lobe effects of nondiffractive beams. Here, we propose double-pulse laser capture microdissection with a needle beam (DPLM-NB) for thick biological samples. A 60-\u03bcm-long needle beam provides an extended axial interaction range, while double-pulse delay control improves energy localization and cutting selectivity. Numerical simulations show that pre-excitation by the first pulse enhances energy coupling in the main-lobe region and weakens the side-lobe-associated response. In 30-\u03bcm-thick frozen mouse brain sections, the method reduces the cutting width from 6.75 to 2.47\u2005\u03bcm and the threshold energy density from 5.02 to 1.96\u2005J\/cm\n                    <jats:sup>2<\/jats:sup>\n                    compared with single-pulse Gaussian-beam cutting. At delays of 1.0\u20131.3\u2005ns, it achieves an average cutting width of 2.63\u2005\u03bcm and threshold energy density of 1.85\u2005J\/cm\n                    <jats:sup>2<\/jats:sup>\n                    with reduced collateral damage, enabling precise microdissection for downstream omics analysis.\n                  <\/jats:p>","DOI":"10.1364\/boe.604195","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T07:00:10Z","timestamp":1780902010000},"page":"3617","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Double-pulse laser capture microdissection with a needle beam for reduced collateral damage in biological samples"],"prefix":"10.1364","volume":"17","author":[{"given":"Simo","family":"Tian","sequence":"first","affiliation":[{"name":"Huazhong University of Science and Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguo","family":"He","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03q648j11","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongshu","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03q648j11","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengqin","family":"Shang","sequence":"additional","affiliation":[{"name":"Huazhong University of Science and Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangning","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03q648j11","id-type":"ROR","asserted-by":"publisher"}]},{"name":"HUST-Suzhou Institute for Brainsmatics, JITRI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5519-6248","authenticated-orcid":true,"given":"Hui","family":"Gong","sequence":"additional","affiliation":[{"name":"HUST-Suzhou Institute for Brainsmatics, JITRI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6725-9311","authenticated-orcid":true,"given":"Qingming","family":"Luo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03q648j11","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4865-7864","authenticated-orcid":true,"given":"Xiaoquan","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03q648j11","id-type":"ROR","asserted-by":"publisher"}]},{"name":"HUST-Suzhou Institute for Brainsmatics, JITRI"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"boe-17-7-3617-R1","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1038\/s43586-025-00453-4","type":"journal-article","volume":"5","author":"Liotta","year":"2025","journal-title":"Nat. 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