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Opt. Soc. Am. A"],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:p>Coherent anti-Stokes Raman scattering (CARS) microspectroscopy has emerged as a powerful technique for probing molecular assemblies with high chemical specificity. However, differentiating proteins within the same family remains a significant challenge. Here, we address the problem of the discrimination between actin and myosin filaments in human muscle. Starting with an inverse problem formulation, we propose a two-step computational resolution. First, the pure CARS spectra of actin and myosin are estimated. Second, these spectra are used to perform a spectral unmixing onto the whole image in order to quantify the relative contribution of each protein at the pixel level. 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All rights, including for text and data mining (TDM), Artificial Intelligence (AI) training, and similar technologies, are reserved.","name":"copyright","label":"Copyright"}]}}