{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:11:10Z","timestamp":1785409870109,"version":"3.56.0"},"reference-count":37,"publisher":"Optica Publishing Group","issue":"6","license":[{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/opg.optica.org\/policies\/opg-tdm-policy.json"}],"funder":[{"name":"European Research Council","award":["101055013"],"award-info":[{"award-number":["101055013"]}],"id":[{"id":"https:\/\/ror.org\/0472cxd90","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"crossref","award":["17046"],"award-info":[{"award-number":["17046"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"crossref"},{"id":"https:\/\/ror.org\/04jsz6e67","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":["opg.optica.org"],"crossmark-restriction":false},"short-container-title":["Opt. Express"],"published-print":{"date-parts":[[2026,3,23]]},"abstract":"<jats:p>Single-molecule fluorescence localization with minimum photon flux imaging (MINFLUX) can achieve localization precisions in the small nanometer range or better under suitable conditions. Potentially adverse conditions, such as a fixed fluorescence dipole or optical aberrations, that could cause systematic localization errors, have received little attention up to now. Here, we study these effects in simulation. We find that biases occur for fluorophores with a fixed absorption dipole tilted out of the imaging plane. These become larger (up to about 25% of the diameter of the circle spanned by the doughnut center positions), the larger the tilt angle gets. As a rule of thumb, the spread in bias is smaller than 5 nm in case the dipole orientation is less than 30\u00b0 out of plane for the typical case of a doughnut probing circle of diameter 100 nm. For freely rotating dipoles, only the primary aberrations, astigmatism and coma, contribute to bias. This bias depends on the position of the fluorophore inside the circular probing area of MINFLUX and can be significantly larger than the localization precision. We show that increasing the number of measurements over the circle from a triangular to a hexagonal pattern is beneficial for reducing bias in all cases. Iterative shrinking of the probing area can eliminate the position-dependent bias completely, but a strong dependence on dipole orientation of the bias at the center of the probing area remains.<\/jats:p>","DOI":"10.1364\/oe.590144","type":"journal-article","created":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T09:00:07Z","timestamp":1772614807000},"page":"10385","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Absorption dipole effects on MINFLUX single molecule localization"],"prefix":"10.1364","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4978-3272","authenticated-orcid":true,"given":"Sjoerd","family":"Stallinga","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02e2c7k09","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxiu","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02e2c7k09","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9215-9307","authenticated-orcid":true,"given":"Bernd","family":"Rieger","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02e2c7k09","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2026,3,13]]},"reference":[{"key":"oe-34-6-10385-R1","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1126\/science.aak9913","type":"journal-article","volume":"355","author":"Balzarotti","year":"2017","journal-title":"Science"},{"key":"oe-34-6-10385-R2","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1038\/s41592-019-0688-0","type":"journal-article","volume":"17","author":"Gwosch","year":"2020","journal-title":"Nat. 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Rieger B. , \u201c Absorption dipole effects on MINFLUX single molecule localization: code ,\u201d TU Delft , 2026 , https:\/\/gitlab.tudelft.nl\/imphys\/ci\/minflux-dipole .","DOI":"10.64898\/2026.01.11.698872"}],"container-title":["Optics Express"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/opg.optica.org\/viewmedia.cfm?URI=oe-34-6-10385&seq=0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T13:08:26Z","timestamp":1778677706000},"score":1,"resource":{"primary":{"URL":"https:\/\/opg.optica.org\/abstract.cfm?URI=oe-34-6-10385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,13]]},"references-count":37,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026]]},"published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.1364\/oe.590144","relation":{"has-preprint":[{"id-type":"doi","id":"10.64898\/2026.01.11.698872","asserted-by":"object"}]},"ISSN":["1094-4087"],"issn-type":[{"value":"1094-4087","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,13]]},"assertion":[{"value":"Optica Publishing Group","name":"publisher","label":"This article is maintained by"},{"value":"https:\/\/doi.org\/10.1364\/OE.590144","name":"articlelink","label":"Crossref DOI link to publisher maintained version"},{"value":"research-article","name":"content_type","label":"Article type"},{"value":"Screened by Similarity Check","name":"cross_check","label":"Similarity check"},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"13 January 2026","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"4 March 2026","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"13 March 2026","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}},{"value":"Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article\u2019s title, journal citation, and DOI.","name":"copyright","label":"Copyright"}]}}