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Lett."],"published-print":{"date-parts":[[2022,11,1]]},"abstract":"<jats:p>Astigmatism imaging is a three-dimensional (3D) single molecule fluorescence microscopy approach that yields super-resolved spatial information on a rapid time scale from a single image. It is ideally suited for resolving structures on a sub-micrometer scale and temporal behavior in the millisecond regime. While traditional astigmatism imaging utilizes a cylindrical lens, adaptive optics enables the astigmatism to be tuned for the experiment. We demonstrate here how the precisions in <jats:italic>x<\/jats:italic>, <jats:italic>y<\/jats:italic>, and <jats:italic>z<\/jats:italic> are inter-linked and vary with the astigmatism, <jats:italic>z<\/jats:italic>-position, and photon level. 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