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To demonstrate the effectiveness of our method, we tracked the heart rates of\n                    <jats:italic>Tg(myl7:GCaMP)s878<\/jats:italic>\n                    transgenic zebrafish across different experimental conditions of temperature changes and drug trials with distinct results. Our robust tracking capabilities of freely swimming fish enable novel, to the best of our knowledge, long-term\n                    <jats:italic>in vivo<\/jats:italic>\n                    fluorescence imaging experiments, allowing for new developmental questions to be analyzed.\n                  <\/jats:p>","DOI":"10.1364\/ol.597720","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T11:03:02Z","timestamp":1781607782000},"page":"5269","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High-throughput heart rate sensing in freely moving zebrafish using a multi-well plate-based fluorescence microscope"],"prefix":"10.1364","volume":"51","author":[{"given":"Kevin","family":"Li","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04bct7p84","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Duke University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0632-0393","authenticated-orcid":true,"given":"Xi","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04bct7p84","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Bagwell","sequence":"additional","affiliation":[{"name":"Duke University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7749-0015","authenticated-orcid":true,"given":"Lucas","family":"Kreiss","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04bct7p84","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6730-0989","authenticated-orcid":true,"given":"Haitao","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04bct7p84","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veton","family":"Saliu","sequence":"additional","affiliation":[{"name":"Ramona Optics, Inc."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lien","family":"B\u00e8gue","sequence":"additional","affiliation":[{"name":"Ramona Optics, Inc."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4657-4603","authenticated-orcid":true,"given":"Mark","family":"Harfouche","sequence":"additional","affiliation":[{"name":"Ramona Optics, Inc."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3829-0168","authenticated-orcid":true,"given":"Michel","family":"Bagnat","sequence":"additional","affiliation":[{"name":"Duke University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roarke","family":"Horstmeyer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04bct7p84","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Ramona Optics, Inc."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"ol-51-18-5269-R1","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1021\/acs.chemrestox.9b00335","type":"journal-article","volume":"33","author":"Cassar","year":"2020","journal-title":"Chem. 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