{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:54:55Z","timestamp":1787021695341,"version":"build-2736575974"},"reference-count":11,"publisher":"Optica Publishing Group","issue":"11","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1364\/OA_License_v2#VOR"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/opg.optica.org\/policies\/opg-tdm-policy.json"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["52575617"],"award-info":[{"award-number":["52575617"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"},{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":["opg.optica.org"],"crossmark-restriction":false},"short-container-title":["Opt. Lett."],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:p>We propose parallel image-scanning autocorrelation-deconvolution microscopy (PISADM), a multifocal-scanning strategy that converts global reconstruction into spatially localized reconstructions. By combining spatially localized multifocal excitation with scanning-position-wise SACD reconstruction and focus-wise pixel reassignment, PISADM reduces the effective accumulation of finite-frame cross terms and improves reconstruction stability in dense regions, thereby enhancing the separability of closely spaced structures. Monte-Carlo simulations show that, at an SBR of 10\u2009dB, PISADM reduces the stable separability limit to approximately 100\u2013110\u2009nm, compared with about 140\u2013150\u2009nm for SACD under the same conditions. Fourier ring correlation analysis shows that PISADM preserves more reliable high-frequency information near the separability limit. In two-dimensional, long-term, and three-dimensional imaging of PCSTAR-labeled microtubules, PISADM consistently provides clearer separation of neighboring filaments while better preserving structural fidelity. These results indicate that PISADM provides a practical strategy for resolving densely packed structures with small spatial separations in super-resolution imaging.<\/jats:p>","DOI":"10.1364\/ol.598988","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T14:00:09Z","timestamp":1778076009000},"page":"3261","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Parallel image-scanning autocorrelation-deconvolution microscopy"],"prefix":"10.1364","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6354-0433","authenticated-orcid":true,"given":"Caini","family":"Xiao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01yqg2h08","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Harbin Institute of Technology)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2016-2865","authenticated-orcid":true,"given":"Xiaoyuan","family":"Du","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01yqg2h08","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Harbin Institute of Technology)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfeng","family":"Fu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangfa","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01yqg2h08","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Harbin Institute of Technology)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Fu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences"},{"name":"University of Chinese Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7708-616X","authenticated-orcid":true,"given":"Junxin","family":"Peng","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences"},{"name":"University of Chinese Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6621-9289","authenticated-orcid":true,"given":"Su","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01yqg2h08","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Harbin Institute of Technology)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1933-0897","authenticated-orcid":true,"given":"Limin","family":"Zou","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01yqg2h08","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Harbin Institute of Technology)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"ol-51-11-3261-R1","doi-asserted-by":"publisher","first-page":"806","DOI":"10.1038\/s41566-023-01234-9","type":"journal-article","volume":"17","author":"Zhao","year":"2023","journal-title":"Nat. Photon."},{"key":"ol-51-11-3261-R2","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1364\/JOSA.62.000055","type":"journal-article","volume":"62","author":"Richardson","year":"1972","journal-title":"J. Opt. Soc. Am."},{"key":"ol-51-11-3261-R3","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1364\/JOSAA.14.000547","type":"journal-article","volume":"14","author":"den Dekker","year":"1997","journal-title":"J. Opt. Soc. Am. A"},{"key":"ol-51-11-3261-R4","doi-asserted-by":"publisher","first-page":"2889","DOI":"10.1364\/OL.38.002889","type":"journal-article","volume":"38","author":"Sheppard","year":"2013","journal-title":"Opt. Lett."},{"key":"ol-51-11-3261-R5","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1038\/nmeth.2025","type":"journal-article","volume":"9","author":"York","year":"2012","journal-title":"Nat. Methods"},{"key":"ol-51-11-3261-R6","doi-asserted-by":"publisher","first-page":"22287","DOI":"10.1073\/pnas.0907866106","type":"journal-article","volume":"106","author":"Dertinger","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ol-51-11-3261-R7","doi-asserted-by":"publisher","first-page":"1308","DOI":"10.1364\/OPTICA.399600","type":"journal-article","volume":"7","author":"Sroda","year":"2020","journal-title":"Optica"},{"key":"ol-51-11-3261-R8","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1038\/nmeth.2448","type":"journal-article","volume":"10","author":"Nieuwenhuizen","year":"2013","journal-title":"Nat. Methods"},{"key":"ol-51-11-3261-R9","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1016\/j.jsb.2013.05.004","type":"journal-article","volume":"183","author":"Banterle","year":"2013","journal-title":"J. Struct. Biol."},{"key":"ol-51-11-3261-R10","doi-asserted-by":"publisher","author":"Xiao","year":"2026","DOI":"10.6084\/m9.figshare.32109709","type":"dataset"},{"key":"ol-51-11-3261-R11","doi-asserted-by":"crossref","unstructured":"Xiao C. Du X. Fu W. et al. , \u201c Parallel image-scanning autocorrelation-deconvolution microscopy ,\u201d GitHub ( 2026 ), https:\/\/github.com\/CainiXiao\/PISADM .","DOI":"10.1364\/OL.598988"}],"container-title":["Optics Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/opg.optica.org\/viewmedia.cfm?URI=ol-51-11-3261&seq=0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T15:56:49Z","timestamp":1780329409000},"score":1,"resource":{"primary":{"URL":"https:\/\/opg.optica.org\/abstract.cfm?URI=ol-51-11-3261"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,1]]},"references-count":11,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2026]]},"published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.1364\/ol.598988","relation":{},"ISSN":["0146-9592","1539-4794"],"issn-type":[{"value":"0146-9592","type":"print"},{"value":"1539-4794","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,1]]},"assertion":[{"value":"Optica Publishing Group","name":"publisher","label":"This article is maintained by"},{"value":"https:\/\/doi.org\/10.1364\/OL.598988","name":"articlelink","label":"Crossref DOI link to publisher maintained version"},{"value":"letter","name":"content_type","label":"Article type"},{"value":"Screened by Similarity Check","name":"cross_check","label":"Similarity check"},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"16 March 2026","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"4 May 2026","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1 June 2026","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}},{"value":"\u00a9 2026 Optica Publishing Group. All rights, including for text and data mining (TDM), Artificial Intelligence (AI) training, and similar technologies, are reserved.","name":"copyright","label":"Copyright"}]}}