{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:55:01Z","timestamp":1790690101551,"version":"4.1.0"},"reference-count":13,"publisher":"Optica Publishing Group","issue":"19","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1364\/OA_License_v2#VOR"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/opg.optica.org\/policies\/opg-tdm-policy.json"}],"funder":[{"name":"Department of Science and Technology, India","award":["INT\/DENMARK\/P-5\/2020"],"award-info":[{"award-number":["INT\/DENMARK\/P-5\/2020"]}]}],"content-domain":{"domain":["opg.optica.org"],"crossmark-restriction":false},"short-container-title":["Opt. Lett."],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:p>\n                    Polluted aquifers harboring harmful bacteria such as Escherichia coli (\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    ) can lead to serious health hazards. Hence, rapid and affordable methods for detection of\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    are important for ensuring public health, especially in rural communities. Photonic crystals (PC) can be used for developing large-scale production of economical, high-sensitivity sensors for use with UV-Vis spectrophotometry. NanoCuvette combines PC slabs with cuvettes, making it suitable for both laboratory and field applications. This study focuses on the development of a protocol for bio-functionalization of a PC waveguide for enhancing the capture and specific detection of\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    bacteria. Insights were obtained from detailed characterization performed at various stages using scanning electron microscopy, fluorescence microscopy, and atomic force microscopy techniques. Subsequently, a bio-functionalization protocol was developed to enable the use of the PC slab in a NanoCuvette for detection of binding of\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    bacteria and for  quantitatively determining the concentrations of\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    . The evaluation was performed using a UV-Vis spectrophotometer based on transmission spectroscopy. This preliminary study reveals promising prospects for rapid\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    detection and quantization in aqueous samples.\n                  <\/jats:p>","DOI":"10.1364\/ol.608763","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T19:00:07Z","timestamp":1787857207000},"page":"5538","update-policy":"https:\/\/doi.org\/10.1364\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nanocuvettes and their application in Escherichia coli detection"],"prefix":"10.1364","volume":"51","author":[{"given":"Moutusi","family":"De","sequence":"first","affiliation":[{"name":"Indian Institute of Technology Bombay"},{"name":"New Panvel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilpi","family":"Maurya","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Bombay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suparna","family":"Mukherji","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Bombay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arpita","family":"De","sequence":"additional","affiliation":[{"name":"Copenhagen Nanosystems"},{"name":"NIL Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soumyo","family":"Mukherji","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Bombay"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"285","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"ol-51-19-5538-R1","doi-asserted-by":"publisher","first-page":"3662","DOI":"10.3390\/w15203662","type":"journal-article","volume":"15","author":"Ravindiran","year":"2023","journal-title":"Water"},{"key":"ol-51-19-5538-R2","doi-asserted-by":"publisher","first-page":"116496","DOI":"10.3390\/w17050745","type":"journal-article","volume":"17","author":"Sarker","year":"2025","journal-title":"Water"},{"key":"ol-51-19-5538-R3","doi-asserted-by":"publisher","first-page":"116496","DOI":"10.1016\/j.watres.2020.116496","type":"journal-article","volume":"188","author":"Chique","year":"2021","journal-title":"Water Res."},{"key":"ol-51-19-5538-R4","doi-asserted-by":"publisher","first-page":"061101","DOI":"10.1063\/1.4928548","type":"journal-article","volume":"107","author":"Hermannsson","year":"2015","journal-title":"Appl. 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All rights, including for text and data mining (TDM), Artificial Intelligence (AI) training, and similar technologies, are reserved.","name":"copyright","label":"Copyright"}]}}