{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:19:21Z","timestamp":1762917561863,"version":"3.38.0"},"reference-count":30,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Appl Spectrosc"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:p> A diffuse reflectance spectroscopy-based method to score fibrosis in paraffin-preserved human liver specimens has been developed and is reported here. Paraffin blocks containing human liver tissue were collected from the General Hospital of Mexico and included in the study with the patients' written consent. The score of liver fibrosis was determined in each sample by two experienced pathologists in a single-blind fashion. Spectral measurements were acquired at 450\u2013750 nm by establishing surface contact between the optical probe and the preserved tissue. According to the histological evaluation, four liver samples showed no evidence of fibrosis and were categorized as F0, four hepatic specimens exhibited an initial degree of fibrosis (F1\u2014F2), five liver specimens showed a severe degree of fibrosis (F3), and six samples exhibited cirrhosis (F4). The human liver tissue showed a characteristic diffuse reflectance spectrum associated with the progressive stages of fibrosis. In the F0 liver samples, the diffuse reflection intensity gradually increased in the wavelength range of 450\u2013750 nm. In contrast, the F1\u2013F2, F3, and F4 specimens showed corresponding 1.5-, 2-, and 5.5-fold decreases in the intensity of diffuse reflectance compared to the F0 liver specimens. At 650 nm, all the stages of liver fibrosis were clearly distinguished from each other with high sensitivity and specificity (92\u2013100%). To our knowledge, this is the first study reporting a distinctive diffuse reflectance spectrum for each stage of fibrosis in paraffin-preserved human liver specimens. These results suggest that diffuse reflectance spectroscopy may represent a complementary tool to liver biopsy for grading fibrosis. <\/jats:p>","DOI":"10.1366\/14-07462","type":"journal-article","created":{"date-parts":[[2014,11,26]],"date-time":"2014-11-26T21:11:10Z","timestamp":1417036270000},"page":"1357-1364","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Diffuse Reflectance Spectroscopy as a Possible Tool to Complement Liver Biopsy for Grading Hepatic Fibrosis in Paraffin-Preserved Human Liver Specimens"],"prefix":"10.1177","volume":"68","author":[{"given":"Diego A.","family":"Fabila-Bustos","sequence":"first","affiliation":[{"name":"Laboratorio de Biofot\u00f3nica, ESIME ZAC, Instituto Polit\u00e9cnico Nacional, M\u00e9xico D.F. 07738, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ursula D.","family":"Arroyo-Camarena","sequence":"additional","affiliation":[{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico, Unidad de Medicina Experimental, Facultad de Medicina, Hospital General de M\u00e9xico \u201cDr. Eduardo Liceaga,\u201d M\u00e9xico D.F. 06726, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda D.","family":"L\u00f3pez-Vancell","sequence":"additional","affiliation":[{"name":"Hospital General de M\u00e9xico \u201cDr. Eduardo Liceaga,\u201d Servicio de Patolog\u00eda, M\u00e9xico D.F. 06726, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco A.","family":"Dur\u00e1n-Padilla","sequence":"additional","affiliation":[{"name":"Hospital General de M\u00e9xico \u201cDr. Eduardo Liceaga,\u201d Servicio de Patolog\u00eda, M\u00e9xico D.F. 06726, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itzel","family":"Azuceno-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico, Unidad de Medicina Experimental, Facultad de Medicina, Hospital General de M\u00e9xico \u201cDr. Eduardo Liceaga,\u201d M\u00e9xico D.F. 06726, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suren","family":"Stolik-Isakina","sequence":"additional","affiliation":[{"name":"Laboratorio de Biofot\u00f3nica, ESIME ZAC, Instituto Polit\u00e9cnico Nacional, M\u00e9xico D.F. 07738, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Ibarra-Coronado","sequence":"additional","affiliation":[{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico, Departamento de Inmunolog\u00eda, Instituto de Investigaciones Biom\u00e9dicas, Ciudad Universitaria 04510, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Blair","family":"Brown","sequence":"additional","affiliation":[{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico, Unidad de Medicina Experimental, Facultad de Medicina, Hospital General de M\u00e9xico \u201cDr. Eduardo Liceaga,\u201d M\u00e9xico D.F. 06726, M\u00e9xico"},{"name":"University of Minnesota, Medical School, 420 Delaware St SE, Minneapolis, MN 55455 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Galileo","family":"Escobedo","sequence":"additional","affiliation":[{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico, Unidad de Medicina Experimental, Facultad de Medicina, Hospital General de M\u00e9xico \u201cDr. Eduardo Liceaga,\u201d M\u00e9xico D.F. 06726, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Manuel","family":"de la Rosa-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Laboratorio de 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