{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:39:55Z","timestamp":1777851595649,"version":"3.51.4"},"reference-count":12,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2011,5,23]],"date-time":"2011-05-23T00:00:00Z","timestamp":1306108800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:p>The sodium-dependent inorganic phosphate transporter NaPi-IIa is expressed in the kidney. Here, the authors used a polyclonal antiserum raised against NaPi-IIa- and NaPi-IIa-deficient mice to characterize its expression in nervous tissue. Western blots showed that a NaPi-IIa immunoreactive band (~90 kDa) was only present in wild-type kidney membranes and not in kidney knockout or wild-type brain membranes. In the water-soluble fraction of wild-type and knockout brains, another band (~50 kDa) was observed; this band was not detected in the kidney. Light and electron microscopic immunohistochemistry using the NaPi-IIa antibodies showed immunolabeling of kidney tubules in wild-type but not knockout mice. In the brain, labeling of presynaptic nerve terminals was present also in NaPi-IIa-deficient mice. This labeling pattern was also produced by the NaPi-IIa preimmune serum. The authors conclude that the polyclonal antiserum is specific toward NaPi-IIa in the kidney, but in the brain, immunolabeling is caused by a cross-reaction of the antiserum with an unknown cytosolic protein that is not present in the kidney. This tissue-specific cross-reactivity highlights a potential pitfall when validating antibody specificity using knockout mouse-derived tissue other than the specific tissue of interest and underlines the utility of specificity testing using preimmune sera.<\/jats:p>","DOI":"10.1369\/0022155411411713","type":"journal-article","created":{"date-parts":[[2011,8,11]],"date-time":"2011-08-11T15:58:17Z","timestamp":1313078297000},"page":"807-812","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["The Sodium-Dependent Inorganic Phosphate Transporter SLC34A1 (NaPi-IIa) Is Not Localized in the Mouse Brain"],"prefix":"10.1177","volume":"59","author":[{"given":"Max","family":"Larsson","sequence":"first","affiliation":[{"name":"Department of Anatomy and Centre for Molecular Biology and Neuroscience, University of Oslo, Oslo, Norway (ML,CM,NCD,VG)"},{"name":"Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden (ML)"},{"name":"Departamento de Gen\u00e9tica y Biolog\u00eda Molecular, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, M\u00e9xico D.F., Mexico (IP-N)"},{"name":"Institute of Physiology, University Z\u00fcrich, Z\u00fcrich, Switzerland (JB)"},{"name":"Department of Neurology, Oslo University Hospital, Oslo, Norway (VG)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilie","family":"Morland","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Centre for Molecular Biology and Neuroscience, University of Oslo, Oslo, Norway (ML,CM,NCD,VG)"},{"name":"Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden (ML)"},{"name":"Departamento de Gen\u00e9tica y Biolog\u00eda Molecular, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, M\u00e9xico D.F., Mexico (IP-N)"},{"name":"Institute of Physiology, University Z\u00fcrich, Z\u00fcrich, Switzerland (JB)"},{"name":"Department of Neurology, Oslo University Hospital, Oslo, Norway (VG)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irais","family":"Poblete-Naredo","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Centre for Molecular Biology and Neuroscience, University of Oslo, Oslo, Norway (ML,CM,NCD,VG)"},{"name":"Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden (ML)"},{"name":"Departamento de Gen\u00e9tica y Biolog\u00eda Molecular, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, M\u00e9xico D.F., Mexico (IP-N)"},{"name":"Institute of Physiology, University Z\u00fcrich, Z\u00fcrich, Switzerland (JB)"},{"name":"Department of Neurology, Oslo University Hospital, Oslo, Norway (VG)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrg","family":"Biber","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Centre for Molecular 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