{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T01:53:39Z","timestamp":1792720419592,"version":"4.5.0"},"reference-count":37,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2012,8,16]],"date-time":"2012-08-16T00:00:00Z","timestamp":1345075200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:p>Quantum dot nanocrystal probes (QDs) have been used for detection of somatostatin hormone in secretory granules of somatostatinoma tumor cells by immunofluorescence light microscopy, super-resolution light microscopy, and immunoelectron microscopy. Immunostaining for all modalities was done using sections taken from an epoxy resin-embedded tissue specimen and a similar labeling protocol. This approach allowed assessment of labeling at light microscopy level before examination at super-resolution and electron microscopy level and was a significant aid in interpretation. Etching of ultrathin sections with saturated sodium metaperiodate was a critical step presumably able to retrieve some tissue antigenicity masked by processing in epoxy resin. Immunofluorescence microscopy of QD-immunolabeled sections showed somatostatin hormone localization in cytoplasmic granules. Some variable staining of tumor gland-like structures appeared related to granule maturity and dispersal of granule contents within the tumor cell cytoplasm. Super-resolution light microscopy demonstrated localization of somatostatin within individual secretory granules to be heterogeneous, and this staining pattern was confirmed by immunoelectron microscopy.<\/jats:p>","DOI":"10.1369\/0022155412459856","type":"journal-article","created":{"date-parts":[[2012,8,16]],"date-time":"2012-08-16T22:34:22Z","timestamp":1345156462000},"page":"832-843","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Quantum Dot Immunocytochemical Localization of Somatostatin in Somatostatinoma by Widefield Epifluorescence, Super-resolution Light, and Immunoelectron Microscopy"],"prefix":"10.1177","volume":"60","author":[{"given":"Murray C.","family":"Killingsworth","sequence":"first","affiliation":[{"name":"Electron Microscopy Laboratory incorporating the Nanobiology Unit of the University of Western Sydney, NSW Health Pathology, Sydney, Australia (MCK,KL,XW,JLCY,CSL)"},{"name":"School of Medicine, University of Western Sydney, Sydney, Australia (MCK,KL,XW,JLCY,CSL)"},{"name":"Faculty of Medicine, University of New South Wales, Sydney, Australia (MCK)"},{"name":"Bosch Institute, University of Sydney, Sydney, Australia (KL,CSL)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Lai","sequence":"additional","affiliation":[{"name":"Electron Microscopy Laboratory incorporating the Nanobiology Unit of the University of Western Sydney, NSW Health Pathology, Sydney, Australia (MCK,KL,XW,JLCY,CSL)"},{"name":"School of Medicine, University of Western Sydney, Sydney, Australia (MCK,KL,XW,JLCY,CSL)"},{"name":"Faculty of Medicine, University of New South Wales, Sydney, Australia (MCK)"},{"name":"Bosch Institute, University of Sydney, Sydney, Australia (KL,CSL)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Electron Microscopy Laboratory incorporating the Nanobiology Unit of the University of Western Sydney, NSW Health Pathology, Sydney, Australia (MCK,KL,XW,JLCY,CSL)"},{"name":"School of Medicine, University of Western Sydney, Sydney, Australia (MCK,KL,XW,JLCY,CSL)"},{"name":"Faculty of Medicine, University of New South Wales, Sydney, Australia (MCK)"},{"name":"Bosch Institute, University of Sydney, Sydney, Australia (KL,CSL)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim L. C.","family":"Yong","sequence":"additional","affiliation":[{"name":"Electron Microscopy Laboratory incorporating the Nanobiology Unit of the University of Western Sydney, NSW Health Pathology, Sydney, Australia (MCK,KL,XW,JLCY,CSL)"},{"name":"School of Medicine, University of Western Sydney, Sydney, Australia (MCK,KL,XW,JLCY,CSL)"},{"name":"Faculty of Medicine, University of New South Wales, Sydney, Australia (MCK)"},{"name":"Bosch Institute, University of Sydney, Sydney, Australia (KL,CSL)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Soon","family":"Lee","sequence":"additional","affiliation":[{"name":"Electron Microscopy Laboratory incorporating the Nanobiology Unit of the University of Western Sydney, NSW Health Pathology, Sydney, Australia (MCK,KL,XW,JLCY,CSL)"},{"name":"School of Medicine, University of Western Sydney, Sydney, Australia (MCK,KL,XW,JLCY,CSL)"},{"name":"Faculty of Medicine, University of New South Wales, Sydney, Australia (MCK)"},{"name":"Bosch Institute, University of Sydney, Sydney, Australia (KL,CSL)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,8,16]]},"reference":[{"key":"bibr1-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.63.1.12"},{"key":"bibr2-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-4328(96)00042-X"},{"key":"bibr3-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1177\/002215549704500117"},{"key":"bibr4-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-4328(98)00010-9"},{"key":"bibr5-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-4328(98)00024-9"},{"key":"bibr6-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2004.02.001"},{"key":"bibr7-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2006.11.006"},{"key":"bibr8-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1016\/0968-4328(95)00008-9"},{"key":"bibr9-0022155412459856","doi-asserted-by":"publisher","DOI":"10.3109\/01913129809032281"},{"key":"bibr10-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2003.10.001"},{"key":"bibr11-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1007\/BF00315744"},{"key":"bibr12-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1177\/37.7.2732456"},{"key":"bibr13-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1038\/nbt994"},{"key":"bibr14-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth791"},{"key":"bibr15-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1177\/002215540104900313"},{"key":"bibr16-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1117\/12.384189"},{"key":"bibr17-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1177\/002215540104900407"},{"key":"bibr18-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1177\/33.12.3934258"},{"key":"bibr19-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1126\/science.1137395"},{"key":"bibr20-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20928"},{"key":"bibr21-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1177\/002215540405200102"},{"key":"bibr22-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1017\/S1431927609991188"},{"key":"bibr23-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1248"},{"key":"bibr24-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1177\/002215549704500501"},{"key":"bibr25-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1177\/28.1.6153194"},{"key":"bibr26-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-294-6_12"},{"key":"bibr27-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1177\/43.2.7529784"},{"key":"bibr28-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1021\/bc900438a"},{"key":"bibr29-0022155412459856","doi-asserted-by":"publisher","DOI":"10.2144\/000112239"},{"key":"bibr30-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2818.2003.01219.x"},{"key":"bibr31-0022155412459856","first-page":"1171","volume":"21","author":"Varikatt W","year":"2006","journal-title":"Histol Histopathol"},{"key":"bibr32-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00815.x"},{"key":"bibr33-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1093\/jmicro\/dfp017"},{"key":"bibr34-0022155412459856","first-page":"217","volume":"30","author":"Yamazaki K","year":"1998","journal-title":"J Submicrosc Cytol Pathol"},{"key":"bibr35-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1177\/002215540305100208"},{"key":"bibr36-0022155412459856","doi-asserted-by":"publisher","DOI":"10.1177\/002215540104900301"},{"key":"bibr37-0022155412459856","first-page":"210","volume":"3","author":"Yong JL","year":"2010","journal-title":"Int J Clin Exp Pathol"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/0022155412459856","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1369\/0022155412459856","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/0022155412459856","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:26:50Z","timestamp":1777501610000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1369\/0022155412459856"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,16]]},"references-count":37,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1369\/0022155412459856"],"URL":"https:\/\/doi.org\/10.1369\/0022155412459856","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,16]]}}}