{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:43:14Z","timestamp":1791117794301,"version":"4.1.0"},"reference-count":33,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T00:00:00Z","timestamp":1418947200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:p>Apoptosis of activated hepatic stellate cells (HSCs) has been verified as a potential mechanism to aid in hepatic fibrosis remission. Earlier research suggests that Septin4_i1 may sensitize hepatocellular carcinoma cells to serum starvation-induced apoptosis. Here, we aimed to investigate the effect of Septin4_i1 on HSC apoptosis and explore the associated signaling pathways. We found that Septin4_i1 can induce apoptosis in LX-2 cells and that this is accompanied by an up-regulation in cleaved-caspase-3 and peroxisome proliferator-activated receptor-\u03b3 (PPAR-\u03b3) expression and a down-regulation in \u03b1-SMA expression. Over-expression of Septin4_i1 reduced phosphorylated Akt and B-cell lymphoma 2 (Bcl-2) expression but had no effect on the expression of p53 and death receptor (DR)-5. The decreased expression of Bcl-2 and the increased expression of cleaved-caspase-3 induced by Sept4_i1 could be reversed by GW501516, a PPAR-\u03b2\/\u03b4 agonist that has been reported by others to enhance Akt signaling. In addition, GW9662, an antagonist of PPAR-\u03b3, could also inhibit apoptosis in LX-2 cells induced by Sept4_i1. In conclusion, our data suggest that Sept4_i1 induces HSC apoptosis by inhibiting Akt and Bcl-2 expression and up-regulating PPAR-\u03b3 expression.<\/jats:p>","DOI":"10.1369\/0022155414567230","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T23:50:12Z","timestamp":1419033012000},"page":"163-169","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Septin4_i1 Regulates Apoptosis in Hepatic Stellate Cells through Peroxisome Proliferator-Activated Receptor-\u03b3\/Akt\/B-Cell Lymphoma 2 Pathway"],"prefix":"10.1369","volume":"63","author":[{"given":"Dandan","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Pathogen Biology, School of Medicine, Nantong University, People\u2019s Republic of China (DZ, JW, XS, JC, YD, JP, TX, YQ, XH, CH)"},{"name":"Laboratory Medicine Center, Affiliated Hospital of Nantong University, People\u2019s Republic of China 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