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The lack of reliable TC-markers has hindered the elucidation of its role. We developed site-specific and phosphorylation-status\u2013specific antibodies against Girdin at tyrosine-1798 (pY1798) and found pY1798 immunostaining of mouse jejunum clearly depicted epithelial cells closely resembling TCs. This study aimed to validate pY1798 as a TC-marker. Double-fluorescence staining of intestines was performed with pY1798 and known TC-markers, for example, hematopoietic-prostaglandin-D-synthase (HPGDS), or doublecortin-like kinase 1 (DCLK1). Odds ratios (ORs) were calculated from cell counts to determine whether two markers were attracting (OR&lt;1) or repelling (OR&gt;1). In consequence, pY1798 signals strongly attracted those of known TC-markers. ORs for HPGDS in mouse stomach, small intestine, and colon were 0 for all, and 0.08 for DCLK1 in human small intestine. pY1798-positive cells in jejunum were distinct from other minor epithelial cells, including goblet, Paneth, and neuroendocrine cells. Thus, pY1798 was validated as a TC-marker. Interestingly, apoptosis inducers significantly increased relative TC frequencies despite the absence of proliferation at baseline. In conclusion, pY1798 is a novel TC-marker. Selective tyrosine phosphorylation and possible resistance to apoptosis inducers implied the activation of certain kinase(s) in TCs, which may become a clue to elucidate the enigmatic roles of TCs.\u2003<\/jats:p>","DOI":"10.1369\/0022155417702586","type":"journal-article","created":{"date-parts":[[2017,4,4]],"date-time":"2017-04-04T18:13:35Z","timestamp":1491329615000},"page":"347-366","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":22,"title":["Tyrosine Phosphorylation of an Actin-Binding Protein Girdin Specifically Marks Tuft Cells in Human and Mouse Gut"],"prefix":"10.1369","volume":"65","author":[{"given":"Daisuke","family":"Kuga","sequence":"first","affiliation":[{"name":"Department of Pathology (DK, KU, SM, AE, NA, MT, MA), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Division of Surgical Oncology, Department of Surgery (DK, MN), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Division of Molecular Pathology, Center for Neurological Disease and Cancer (NA, MT), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Department of Endocrinology and Diabetes (MA), Nagoya University Graduate School of Medicine, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Ushida","sequence":"additional","affiliation":[{"name":"Department of Pathology (DK, KU, SM, AE, NA, MT, MA), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Division of Surgical Oncology, Department of Surgery (DK, MN), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Division of Molecular Pathology, Center for Neurological Disease and Cancer (NA, MT), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Department of Endocrinology and Diabetes (MA), Nagoya University Graduate School of Medicine, Nagoya, 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Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Division of Molecular Pathology, Center for Neurological Disease and Cancer (NA, MT), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Department of Endocrinology and Diabetes (MA), Nagoya University Graduate School of Medicine, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoya","family":"Asai","sequence":"additional","affiliation":[{"name":"Department of Pathology (DK, KU, SM, AE, NA, MT, MA), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Division of Surgical Oncology, Department of Surgery (DK, MN), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Division of Molecular Pathology, Center for Neurological Disease and Cancer (NA, MT), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Department of Endocrinology and Diabetes (MA), Nagoya University Graduate School of Medicine, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Nagino","sequence":"additional","affiliation":[{"name":"Department of Pathology (DK, KU, SM, AE, NA, MT, MA), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Division of Surgical Oncology, Department of Surgery (DK, MN), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Division of Molecular Pathology, Center for Neurological Disease and Cancer (NA, MT), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Department of Endocrinology and Diabetes (MA), Nagoya University Graduate School of Medicine, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahide","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Pathology (DK, KU, SM, AE, NA, MT, MA), Nagoya University Graduate School of Medicine, Nagoya, Japan"},{"name":"Division of Surgical Oncology, Department of Surgery (DK, 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