{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:45:08Z","timestamp":1777851908715,"version":"3.51.4"},"reference-count":54,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T00:00:00Z","timestamp":1508716800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:p>Connective tissue growth factor (CTGF, also named CCN2) plays an important role in the development of tubulointerstitial fibrosis, which most critically determines the progression to end-stage renal failure in autosomal-dominant polycystic kidney disease (ADPKD), the most common genetically caused renal disease. We determined CTGF expression in a well-characterized animal model of human ADPKD, the PKD\/Mhm (cy\/+) rat. Kidneys of 12 weeks old (cy\/+) as well as (+\/+) non-affected rats were analyzed for CTGF RNA and protein expression by RT-PCR, Northern and Western blot analyses, in situ hybridization, and IHC. Besides the established expression of CTGF in glomerular cells in kidneys of wild-type (+\/+) animals, in (cy\/+) rats, CTGF mRNA and protein were robustly expressed in interstitial, stellate-shaped cells, located in a scattered pattern underlying the cystic epithelium and in focal areas of advanced tubulointerstitial remodeling. Renal CTGF mRNA and protein expression levels were significantly higher in (cy\/+) rats compared with their (+\/+) littermates. Detection of CTGF expression in cells adjacent to cystic epithelium and in areas of marked fibrosis suggests a role in the local response to cyst development and indicates that CTGF may be a relevant factor contributing to tubulointerstitial fibrosis in polycystic kidney disease.<\/jats:p>","DOI":"10.1369\/0022155417735513","type":"journal-article","created":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T16:44:28Z","timestamp":1508777068000},"page":"743-755","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["CTGF Is Expressed During Cystic Remodeling in the PKD\/Mhm (cy\/+) Rat Model for Autosomal-Dominant Polycystic Kidney Disease (ADPKD)"],"prefix":"10.1369","volume":"65","author":[{"given":"Stefan","family":"Gauer","sequence":"first","affiliation":[{"name":"Department of Nephrology, Medical Clinic III, University Hospital Frankfurt, Goethe-University Frankfurt, Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne","family":"Holzmann","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Medical Clinic III, University Hospital Frankfurt, Goethe-University Frankfurt, Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"Kr\u00e4nzlin","sequence":"additional","affiliation":[{"name":"Medical Research Center, University of Heidelberg, Mannheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigrid C.","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Medical Research Center, University of Heidelberg, Mannheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norbert","family":"Gretz","sequence":"additional","affiliation":[{"name":"Medical Research Center, University of Heidelberg, Mannheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingeborg A.","family":"Hauser","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Medical Clinic III, University Hospital Frankfurt, Goethe-University Frankfurt, Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarete","family":"Goppelt-Struebe","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension, Medical Clinic 4, University of Erlangen-Nuremberg, Erlangen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helmut","family":"Geiger","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Medical Clinic III, University Hospital Frankfurt, Goethe-University Frankfurt, Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Oberm\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Medical Clinic III, University Hospital Frankfurt, Goethe-University Frankfurt, Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,10,23]]},"reference":[{"key":"bibr1-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006121387"},{"key":"bibr2-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2015.59"},{"key":"bibr3-0022155417735513","first-page":"S93","volume":"63","author":"Grantham JJ.","year":"1997","journal-title":"Kidney Int Suppl"},{"key":"bibr4-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/11.supp6.46"},{"key":"bibr5-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005060601"},{"key":"bibr6-0022155417735513","unstructured":"Inbred strains of rat: PKD [Internet]. http:\/\/www.informatics.jax.org\/inbred_strains\/rat\/docs\/PKD.shtml."},{"key":"bibr7-0022155417735513","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.100569"},{"issue":"3","key":"bibr8-0022155417735513","first-page":"F357","volume":"273","author":"Oberm\u00fcller N","year":"1997","journal-title":"Am J Physiol"},{"key":"bibr9-0022155417735513","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17060802"},{"key":"bibr10-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2014.137"},{"key":"bibr11-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201006173"},{"key":"bibr12-0022155417735513","first-page":"59","volume":"7","author":"Chaqour B.","year":"2013","journal-title":"Trends Dev Biol"},{"key":"bibr13-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1007\/s12079-016-0324-z"},{"key":"bibr14-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.1998.00820.x"},{"key":"bibr15-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2613.2012.00845.x"},{"key":"bibr16-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2017.03.029"},{"issue":"1","key":"bibr17-0022155417735513","first-page":"61","volume":"8","author":"Kothapalli D","year":"1997","journal-title":"Cell Growth Differ"},{"key":"bibr18-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000051600.53134.B9"},{"key":"bibr19-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2014.184"},{"key":"bibr20-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000092913.60428.E6"},{"key":"bibr21-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2012.06.015"},{"key":"bibr22-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014145"},{"key":"bibr23-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008080882"},{"key":"bibr24-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053780"},{"key":"bibr25-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1159\/000430160"},{"key":"bibr26-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1002\/path.2856"},{"key":"bibr27-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1177\/030098588902600302"},{"key":"bibr28-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfm410"},{"key":"bibr29-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"bibr30-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1177\/39.8.1856454"},{"key":"bibr31-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1042\/CS20140264"},{"key":"bibr32-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.217737"},{"key":"bibr33-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/15.3.296"},{"key":"bibr34-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2011.06.012"},{"key":"bibr35-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.280.3.F540"},{"key":"bibr36-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2008.00416.x"},{"key":"bibr37-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfm697"},{"key":"bibr38-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.06.035"},{"key":"bibr39-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-013-1642-6"},{"key":"bibr40-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.109.136531"},{"key":"bibr41-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2012.03.021"},{"key":"bibr42-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028915"},{"key":"bibr43-0022155417735513","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.080652"},{"key":"bibr44-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1159\/000355429"},{"key":"bibr45-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1402685"},{"key":"bibr46-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1402686"},{"key":"bibr47-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.764449"},{"key":"bibr48-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2005.00337.x"},{"key":"bibr49-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1159\/000090504"},{"key":"bibr50-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.00776.x"},{"key":"bibr51-0022155417735513","doi-asserted-by":"publisher","DOI":"10.4161\/cc.20717"},{"key":"bibr52-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.01.004"},{"key":"bibr53-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01045"},{"key":"bibr54-0022155417735513","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2016080826"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/0022155417735513","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1369\/0022155417735513","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/0022155417735513","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:27:44Z","timestamp":1777501664000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1369\/0022155417735513"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,23]]},"references-count":54,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1369\/0022155417735513"],"URL":"https:\/\/doi.org\/10.1369\/0022155417735513","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,23]]}}}