{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:45:09Z","timestamp":1777851909890,"version":"3.51.4"},"reference-count":50,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2017,11,8]],"date-time":"2017-11-08T00:00:00Z","timestamp":1510099200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:p>This study was aimed to understand the mechanism of persistent cardiac myocyte (CM) survival in myocardial infarction (MI) scars. A transmural MI was induced in 12-month-old Sprague\u2013Dawley rats by permanent coronary artery ligation. The hearts were collected 3 days, 1, 2, 4, 8, and 12 weeks after MI and evaluated with histology, immunohistochemistry, and quantitative morphometry. Vasculature patency was assessed in 4-, 8-, and 12-week-old scars by infusion of 15-micron microspheres into the left ventricle before euthanasia. The infarcted\/scarred area has a small continually retained population of surviving CMs in subendocardial and subepicardial regions. Surprisingly, whereas the transverse area of subepicardial CMs remained relatively preserved or even enlarged over 12 post-MI weeks, subendocardial CMs underwent progressive atrophy. Nevertheless, the fractional volume of viable CMs remained comparable in mature scars 4, 8, and 12 weeks after MI (3.6 \u00b1 0.4%, 3.4 \u00b1 0.5%, and 2.5 \u00b1 0.3%, respectively). Despite the opposite dynamics of changes in size, CMs of both regions displayed sarcomeres and gap junctions. Most importantly, surviving CMs were always accompanied by patent microvessels linked to a venous network composed of Thebesian veins, intramural sinusoids, and subepicardial veins. Our findings reveal that long-term survival of CMs in transmural post-MI scars is sustained by a local microcirculatory bed.<\/jats:p>","DOI":"10.1369\/0022155417741640","type":"journal-article","created":{"date-parts":[[2017,11,8]],"date-time":"2017-11-08T14:31:48Z","timestamp":1510151508000},"page":"99-120","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Preservation of Functional Microvascular Bed Is Vital for Long-Term Survival of Cardiac Myocytes Within Large Transmural Post-Myocardial Infarction Scar"],"prefix":"10.1369","volume":"66","author":[{"given":"Colleen","family":"Nofi","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yevgen","family":"Bogatyryov","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduard I.","family":"Dedkov","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,11,8]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2006.8.1907."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c140067."},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10741-010-9181-7."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1054\/jcaf.2002.129270."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.81.4.1161"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2015.12.006."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.02.012."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2015.11.002."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1671-5411.2013.02.003."},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0066-782X2009001000018"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.65.7.1446"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-195411000-00006"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.12822."},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1369\/0022155413499794."},{"issue":"1","key":"e_1_3_3_16_2","first-page":"57","article-title":"Experimental myocardial infarction in the rat: qualitative and quantitative changes during pathologic evolution","volume":"90","author":"Fishbein MC","year":"1978","unstructured":"Fishbein MC, Maclean D, Maroko PR. Experimental myocardial infarction in the rat: qualitative and quantitative changes during pathologic evolution. Am J Pathol. 1978;90(1):57\u201370.","journal-title":"Am J Pathol"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-002-0365-8."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000013782.76324.92"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.104.527937."},{"issue":"4","key":"e_1_3_3_20_2","first-page":"179","article-title":"Characterization of myocardial infarcts in the rat","volume":"104","author":"Spadaro J","year":"1980","unstructured":"Spadaro J, Fishbein MC, Hare C, Pfeffer MA, Maroko PR. Characterization of myocardial infarcts in the rat. Arch Pathol Lab Med. 1980;104(4):179\u201383.","journal-title":"Arch Pathol Lab Med"},{"issue":"5","key":"e_1_3_3_21_2","first-page":"613","article-title":"The distribution of ischaemia in perfused Wistar rat hearts following coronary artery occlusion","volume":"66","author":"Taira Y","year":"1985","unstructured":"Taira Y, Kanaide H, Nakamura M. The distribution of ischaemia in perfused Wistar rat hearts following coronary artery occlusion. Br J Exp Pathol. 1985;66(5):613\u201321.","journal-title":"Br J Exp Pathol"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/21.10.737"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1997.0388."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.88.6.2872"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.260943."},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63598-5."},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1700620103"},{"issue":"1","key":"e_1_3_3_28_2","first-page":"111","article-title":"The histologic border zone of acute myocardial infarction\u2014islands or peninsulas?","volume":"92","author":"Factor SM","year":"1978","unstructured":"Factor SM, Sonnenblick EH, Kirk ES. The histologic border zone of acute myocardial infarction\u2014islands or peninsulas? Am J Pathol. 1978;92(1):111\u201324.","journal-title":"Am J Pathol"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.67.1.117"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050983"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(01)02642-X"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.68.3.518"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(90)92832-M"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.48.5.640"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2005.04.056."},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.21081."},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.93.2.349"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.75.1.299"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2006.08.006."},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-007-9445-2."},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01109.2004."},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1139\/Y09-011."},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10741-007-9040-3."},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00001.2005."},{"issue":"3","key":"e_1_3_3_45_2","first-page":"321","article-title":"Healing process in the marginal zone of an experimental myocardial infarct. Findings in the surviving cardiac muscle cells","volume":"62","author":"Dusek J","year":"1971","unstructured":"Dusek J, Rona G, Kahn DS. Healing process in the marginal zone of an experimental myocardial infarct. Findings in the surviving cardiac muscle cells. Am J Pathol. 1971;62(3):321\u201338.","journal-title":"Am J Pathol"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00063.2008."},{"issue":"2","key":"e_1_3_3_47_2","first-page":"98","article-title":"Myocardial venous drainage: from anatomy to clinical use","volume":"25","author":"Echeverri D","year":"2013","unstructured":"Echeverri D, Cabrales J, Jimenez A. Myocardial venous drainage: from anatomy to clinical use. J Invasive Cardiol. 2013;25(2):98\u2013105.","journal-title":"J Invasive Cardiol"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-4975(95)00721-0."},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2353(200103)14:2<102::AID-CA1018>3.0.CO;2-4."},{"key":"e_1_3_3_50_2","first-page":"1363","article-title":"Scanning electron microscopy of Thebesian ostia (microdissection by ultrasonication: enzymatic digestion)","volume":"1986","author":"Rosinia FA","unstructured":"Rosinia FA, Low FN. Scanning electron microscopy of Thebesian ostia (microdissection by ultrasonication: enzymatic digestion). Scan Electron Microsc. 1986(Pt 4):1363\u20139.","journal-title":"Scan Electron Microsc"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000068377.01063.79"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/0022155417741640","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1369\/0022155417741640","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/0022155417741640","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:27:46Z","timestamp":1777501666000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1369\/0022155417741640"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,8]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1369\/0022155417741640"],"URL":"https:\/\/doi.org\/10.1369\/0022155417741640","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,8]]}}}