{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:45:22Z","timestamp":1777851922061,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T00:00:00Z","timestamp":1526342400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No. 81300826 [Y. B. Z.]"],"award-info":[{"award-number":["No. 81300826 [Y. B. Z.]"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No. 81570917 [C.F.D]"],"award-info":[{"award-number":["No. 81570917 [C.F.D]"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>Intratympanic gentamicin (ITG) has been used to treat refractory M\u00e9ni\u00e8re\u2019s disease. Disequilibrium after ITG was still a challenge for some patients, and the underlying mechanism is poorly understood. Our previous study demonstrated that gentamicin distributed in the bilateral vestibular efferent neurons (VEN) after ITG; however, does it lead to VEN damage and cause further disequilibrium in patients following ITG? In this study, we observed severe damaged gentamicin-positive neurons of VEN and severe fractured myelin layer plates around neural fibers when viewed under transmission electron microscopy at day 3 after ITG. At day 30, neurons of VEN presented with relatively normal structures. Compared with the control group, the total number of choline acetyltransferase (CHAT) immunolabeling neurons in bilateral VEN showed a significant decrease both at day 3 and day 30. However, there was no significant difference in the total number of CHAT immunolabeling neurons between day 3 and day 30. It indicates that gentamicin is not only retrogradely transported into bilateral VEN, but also results in the degeneration of VEN after ITG. These findings may be related to patients\u2019 disequilibrium symptom after ITG. Furthermore, we speculate that VEN may play a role in vestibular compensation.<\/jats:p>","DOI":"10.1369\/0022155418773746","type":"journal-article","created":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T11:42:07Z","timestamp":1526384527000},"page":"801-812","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["The Degeneration of the Vestibular Efferent Neurons After Intratympanic Gentamicin Administration"],"prefix":"10.1369","volume":"66","author":[{"given":"Qianru","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Otology and Skull Base Surgery, Hearing Research Key Lab of Health Ministry of China, Eye & Ear Nose and Throat Hospital, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yibo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otology and Skull Base Surgery, Hearing Research Key Lab of 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