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Using the optimized CuAAC conditions, we have devised a highly sensitive immunostaining technique, based on the tyramide signal amplification\/catalyzed reporter deposition (TSA\/CARD) method with a novel alkyne tyramide substrate. The described method offers improved detection threshold compared to conventional immunofluorescent staining and produces significantly lower non-specific background than TSA\/CARD with fluorescent tyramides.<\/jats:p>","DOI":"10.1369\/0022155419864113","type":"journal-article","created":{"date-parts":[[2019,7,11]],"date-time":"2019-07-11T10:46:46Z","timestamp":1562842006000},"page":"771-782","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining"],"prefix":"10.1369","volume":"67","author":[{"given":"Stanislav A.","family":"Antonov","sequence":"first","affiliation":[{"name":"Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina V.","family":"Novosadova","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey G.","family":"Kobylansky","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vyacheslav Z.","family":"Tarantul","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor A.","family":"Grivennikov","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,7,11]]},"reference":[{"key":"bibr1-0022155419864113","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2010.957191."},{"key":"bibr2-0022155419864113","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm433."},{"key":"bibr3-0022155419864113","unstructured":"Nuovo GJ. 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